Encapsulation of nodal segments of Cassia angustifolia Vahl. for short-term storage and germplasm exchange

• Autorzy: Parveen S. , Shahzad A.
• Języki publikacji: EN

Abstrakty

EN

The present study described the encapsulation of nodal segments of Cassia angustifolia Vahl. excised from 1-month-old in vitro raised cultures for short-term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were obtained through a combination of 3 % sodium alginate and 100 mM calcium chloride. The maximum conversion response (94 %) of encapsulated beads was obtained in Murashige and Skoog’s medium (MS medium) supplemented with 2.5 μM benzyladenine (BA) and 0.4 μM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated and nonencapsulated nodal segments were also stored at 4°C for different time periods (0, 1, 2, 4, 6 and 8 weeks). The regenerated microshoots were best rooted in optimized rooting medium that comprised half-strength MS + 1.0 μM indole-3-butyric acid (IBA) + 5.0 μM phloroglucinol (PG) for the production of complete plantlets. The regenerated plantlets were successfully hardened and acclimatized in natural conditions with 70 % survival rate.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 03
• Opis fizyczny: p.635-640,fig.,ref.

Twórcy

autor

Parveen S.

• Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202 002, India

autor

Shahzad A.

• Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202 002, India

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Identyfikatory

DOI

10.1007/s11738-013-1441-4