Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 03 |

Tytuł artykułu

Encapsulation of nodal segments of Cassia angustifolia Vahl. for short-term storage and germplasm exchange

Warianty tytułu

Języki publikacji



The present study described the encapsulation of nodal segments of Cassia angustifolia Vahl. excised from 1-month-old in vitro raised cultures for short-term conservation and propagation. Various concentrations and combinations of gelling matrix (sodium alginate) and complexing agents (calcium chloride) were tested to prepare uniform beads. The ideal beads were obtained through a combination of 3 % sodium alginate and 100 mM calcium chloride. The maximum conversion response (94 %) of encapsulated beads was obtained in Murashige and Skoog’s medium (MS medium) supplemented with 2.5 μM benzyladenine (BA) and 0.4 μM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated and nonencapsulated nodal segments were also stored at 4°C for different time periods (0, 1, 2, 4, 6 and 8 weeks). The regenerated microshoots were best rooted in optimized rooting medium that comprised half-strength MS + 1.0 μM indole-3-butyric acid (IBA) + 5.0 μM phloroglucinol (PG) for the production of complete plantlets. The regenerated plantlets were successfully hardened and acclimatized in natural conditions with 70 % survival rate.

Słowa kluczowe








Opis fizyczny



  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202 002, India
  • Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, UP, 202 002, India


  • Adriani M, Piccioni E, Standardi A (2000) Effect of different treatments on the conversion of ‘Hayward’ kiwifruit synthetic seeds to whole plants following encapsulation of in vitro derived buds. New Zeal J Crop Hort 28:59–67.
  • Agrawal V, Sardar PR (2003) In vitro organogenesis and histomorphological investigations in senna (Cassia angustifolia)—a medicinally valuable shrub. Physiol Mol Biol Plants 9:131–140.
  • Agrawal V, Sardar PR (2006) In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli. Biol Plant 50:118–122.
  • Agrawal V, Sardar PR (2007) In vitro regeneration through somatic embryogenesis and organogenesis using cotyledons of Cassia angustifolia Vahl. In Vitro Cell Dev Biol-Plant 43:585–592.
  • Ahmad N, Anis M (2010) Direct plant regeneration from encapsulated nodal segments of Vitex negundo. Biol Plant 54:748–752.
  • Anonymous (1992) The wealth of India: a dictionary of Indian raw materials and industrial products (Vol III). CSIR, New Delhi, pp 354–363.
  • Chand S, Singh AK (2004) Plant regeneration from encapsulated nodal segments of Dalbergia sissoo Roxb., a timber yielding leguminous tree species. J Plant Physiol 161:237–243.
  • Faisal M, Anis M (2007) Regeneration of plants from alginate encapsulated shoots of Tylophora indica (Burm.f.) Merrill, an endangered medicinal plant. J Hort Sci Biotechnol 82:351–354.
  • Faisal M, Ahamd N, Anis M (2006) In vitro plant regeneration from alginate encapsulated microcuttings of Rauvolfia tetraphylla L. Am Eur J Aric Environ Sci 1:1–6.
  • Hung CD, Trueman SJ (2011) Encapsulation technology for short-term preservation and germplasm distribution of the African mahogany Khaya senegalensis. Plant Cell Tiss Organ Cult 107:397–405.
  • Hung CD, Trueman SJ (2012) Alginate encapsulation of shoot tips and nodal segments for short-term storage and distribution of the eucalypt Corymbia torelliana × C. citriodora. Acta Physiol Plant 34:117–128.
  • Lata H, Chandra S, Khan IA, ElSohly MA (2009) Propagation through alginate encapsulation of axillary buds of Cannabis sativa L.—an important medicinal plant. Physiol Mol Biol Plants 15:79–86.
  • Lata H, Chandra S, Mehmedic Z, Khan IA, El-Sohly MA (2012) In vitro germplasm conservation of high D9-tetrahydrocannabinol yielding elite clones of Cannabis sativa L. under slow growth conditions. Acta Physiol Plant 34:743–750.
  • Lulsdorf MM, Tautorus TE, Kikcio SI, Bethuna TD, Dunstan DI (1993) Germination of encapsulated embryos of interior spruce (Picea glauca engelmannii complex) and black spruce (Picea mariana Mill.). Plant Cell Rep 12:385–389.
  • Mandal J, Pattnaik S, Chand PK (2000) Alginate encapsulation of axillary buds of Ocimum americanum L. (Hoary Basil), O. basilicum L. (Sweet Basil), O. gratissimum L. (Shrubby Basil), and O. sanctum L. (Sacred Basil). In Vitro Cell Dev Biol-Plant 36:287–292.
  • Micheli M, Hafiz IA, Standardi A (2007) Encapsulation of in vitro derived explants of olive (Olea europaea L. cv. Moraiolo) II. Effects of storage on capsule and derived shoots performance. Sci Hort 113:286–292.
  • Mishra J, Singh M, Palni LMS, Nandi SK (2011) Assessment of genetic fidelity of encapsulated microshoots of Picrorhiza kurrooa. Plant Cell Tiss Organ Cult 104:181–186.
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497.
  • Naik SK, Chand PK (2006) Nutrient alginate encapsulation of in vitro nodal segments of pomegranate (Punica granatum L.) for germplasm distribution and exchange. Sci Hort 108:247–252.
  • Parveen S, Shahzad A (2011) A micropropagation protocol for Cassia angustifolia Vahl. from root explants. Acta Physiol Plant 33:789–796.
  • Parveen S, Shahzad A, Saema S (2010) In vitro plant regeneration system for Cassia siamea Lam., a leguminous tree of economic importance. Agrofor Syst 80:109–116.
  • Parveen S, Shahzad A, Anis M (2012) Enhanced shoot organogenesis in Cassia angustifolia Vahl.—a difficult-to-root drought resistant medicinal shrub. J Plant Biochem Biotechnol 21:213–219.
  • Piccioni E, Standardi A (1995) Encapsulation of micropropagated buds of six woody species. Plant Cell Tiss Organ Cult 42:221–226.
  • Rai MK, Jaiswal VS, Jaiswal U (2008) Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange. Sci Hort 118:33–38.
  • Rai MK, Asthana P, Singh SK, Jaiswal VS, Jaiswal U (2009) The encapsulation technology in fruit plants—a review. Biotechnol Adv 27:671–679.
  • Rao PS, Bapat VA (1993) Micropropagation of sandalwood (Santalum album L.) and mulberry (Morus indica L.). In: Ahuja MR (ed) Micropropagation of woody plants. Kluwer, Dordrecht, pp 317–345.
  • Reddy MC, Murthy KSR, Pullaiah T (2012) Synthetic seeds: a review in agriculture and forestry. Afr J Biotechnol 11:14254–14275.
  • Rihan HZ, Al-Issawi M, Burchett S, Fuller MP (2011) Encapsulation of cauliflower (Brassica oleracea var botrytis) microshoots as artificial seeds and their conversion and growth in commercial substrates. Plant Cell Tiss Organ Cult 107:243–250.
  • Sharma S, Shahzad A (2012) Encapsulation technology for short-term storage and conservation of a woody climber, Decalepis hamiltonii Wight and Arn. Plant Cell Tiss Organ Cult 111:191–198.
  • Sharma S, Shahzad A, Teixeira da Silva JA (2013) Synseed technology—a complete synthesis. Biotechnol Adv 31:186–207.
  • Shrivastava V, Khan SA, Banerjee S (2009) An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following 6 months of storage. Plant Cell Tiss Organ Cult 99:193–198.
  • Siddique I, Anis M (2007) In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiol Plant 29:233–238.
  • Siddique I, Anis M, Aref IM (2010) In vitro adventitious shoot regeneration via indirect organogenesis from petiole explants of Cassia angustifolia Vahl.—a potential medicinal plant. Appl Biochem Biotechnol 162:2067–2074.
  • Singh AK, Varshney R, Sharma M, Agarwal SS, Bansal KC (2006) Regeneration of plants from alginate-encapsulated shoot tips of Withania somnifera (L.) Dunal, a medicinally important plant species. J Plant Physiol 163:220–223.
  • Singh SK, Rai MK, Asthana P, Sahoo L (2010) Alginate-encapsulation of nodal segments for propagation, short-term conservation and germplasm exchange and distribution of Eclipta alba (L.). Acta Physiol Plant 32:607–610.
  • Soneji JR, Rao PS, Mhatre M (2002) Germination of synthetic seeds of pineapple (Ananas comosus L. Merr.). Plant Cell Rep 20:891–894.
  • Tsvetkov I, Hausman JF (2005) In vitro regeneration from alginate encapsulated microcuttings of Quercus sp. Sci Hort 103:503–507.

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.