The influence of growth regulators on dahlia propagation in tissue culture and acclimatization of plants in ex vitro conditions

• Autorzy: Marcinek B. , Parzymies M. , Poniewozik M. , Kozak D. , Durlak W.
• Języki publikacji: EN

Abstrakty

EN

The aim of the work was to evaluate the influence of cytokinins on in vitro propagated dahlia and their consequent effect on acclimatization. Plant material consisted of shoot tips and nodes. Among the three cytokinins, benzyladenine, kinetin and 2-isopentenyl-adenine, only BA effectively stimulated the shoot multiplication from axiliary buds. The highest multiplication rate was obtained from nodes in the presence of 0.25– 0.5 mg·dm–3 BA. Higher concentrations shortened the internodes and decreased the leaf blades and growth of callus. 1 mg·dm–3 of KIN and 2iP positively influenced the shoot growth and size of leaves. Gibberellic acid (GA3) used with BA increased the number of auxillary shoots. The best quality shoots and the highest multiplication rate were obtained when 2 mg·dm–3 BA was used with 5 mg·dm–3 GA3. Cytokinins affected the rooting and acclimatization ex vitro. Dahlia shoots multiplicated in the presence of 1 mg·dm–3 KIN or 2iP rooted faster in the soil and 100% survived in field, while those from 1 mg·dm–3 BA media rooted slowly, had shorter shoots and only 60% of them survived. Plants bloomed after 11–12 weeks in the field. Dahlia plants that had been multiplicated in the presence of KIN had larger diameter and fresh weight in the field. BA and 2iP positively influenced the flower diameter, length of flower stalk and a number of the first-order shoots.

Słowa kluczowe

EN

plant cultivation; Dahlia; Dahlia x cultorum; dahlia; ornamental plant; flowering plant; tuberous plant; tuberous root; plant acclimatization; growth regulator; cytokinin; gibberellic acid; micropropagation; tissue culture; ex vitro method; microcutting

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2019
• Tom: 18
• Numer: 5
• Opis fizyczny: p.49-61,fig.,ref.

Twórcy

autor

Marcinek B.

• Institute of Horticulture Production, Subdepartment of Ornamental Plants And Dendrology, University of Life Sciences In Lublin, Lublin, Poland

autor

Parzymies M.

• Institute of Horticulture Production, Subdepartment of Ornamental Plants And Dendrology, University of Life Sciences In Lublin, Lublin, Poland

autor

Poniewozik M.

• Institute of Horticulture Production, Subdepartment of Ornamental Plants And Dendrology, University of Life Sciences In Lublin, Lublin, Poland

autor

Kozak D.

• Institute of Horticulture Production, Subdepartment of Ornamental Plants And Dendrology, University of Life Sciences In Lublin, Lublin, Poland

autor

Durlak W.

• Institute of Horticulture Production, Subdepartment of Ornamental Plants And Dendrology, University of Life Sciences In Lublin, Lublin, Poland

Bibliografia

• Al-Mizory, L.S.M. (2013). Effect of different concentration of cytokinins, carbon source and agar on in vitro propagation of Dahlia sp. through one single node. J. Life Sci., 7(10), 1103–1112.
• Dalda Şekerci, A., Gülşen, O. (2016). Overview of dahlia breeding. Sci. Papers Ser. B, Horticulture, 60, 199–204.
• Fatima, B., Usman, M., Ashraf, T., Waseem, R., Ali, M.A. (2007). In vitro shoot regeneration from cotyledon and hypocotyl explants of dahlia cultivars. Pak. J. Agric. Sci., 44(2), 312–316.
• Hernandez-Perez, F., Mejila-Munoz, J.M. (1994). Dahlia (Dahlia variabilis Cav.) micropropagation. Rev. Chapingo Ser. Hortic., 1(1), 63–66.
• Hetman, J., Łukawska-Sudoł, S., Pudelska, K., Parzymies, M. (2017). The effect of cutting metod on footing of Dahlia pinnata Cav. cutting. Acta Sci. Pol. Hortorum Cultus, 16(2), 149–160.
• Ibrahim, M.A., Daraj, I.A. (2015a). Micropropagation of dahlia plants Dahlia variabilis Wild (Desf.). Effect of explant and plant growth regulators on shoot regeneration and growth. Adv Agric. Bot., 7(1), 1–6.
• Ibrahim, M.A., Daraj, I.A. (2015b). Micro propagation of dahlia plants (Dahlia variabilis). Direct and indirect organogenesis techniques. Adv. Agric. Bot., 7(1), 28–35.
• Ibrahim, M.A., Daraj, I.A. (2015c). Effect of some treatments on seeds germination, shoots multiplication and rooting of dahlia plants via in vitro culture. Swed. J. Plant Biotechnol., 13–21. Website: www.sjpb.se.
• Jiménez Mariña, L. (2015). El cultivo de la Dalia. Cultiv. Trop., 36(1), 107–115.
• Ju, Z.X. (2007). Study on the technology of tissue culture and rapid reproducing of Dahlia pinnata F1. J. Anhui Agric. Sci., 35(21), 6374.
• Otani, Y., Endo, C., Chin, D.P., Mii, M. (2013). Highly efficient system for plant regeneration from leaf and stem explants in Dahlia. Plant Biotechnol., 30(2), 141–146.
• Pappu, H.R., Wyatt, S.D., Druffel, K.L. (2005). Dahlia mosaic virus: molecular detection and distribution in dahlia in the US. Hort. Sci., 40, 697–699.
• Priyanka, R., Varghese, T.M., Yadav, N.R., Chowdhury, V.K., Chowdhury, J.B. (2001). In vitro studies in Dahlia variabilis. Ann. Agric. Bio. Res., 6(1), 9–13.
• Pudelska, K., Hetman, J., Łukawska-Sudoł, S., Parzymies, M. (2015). The efficiency of mother crowns and quality of soft cuttings of a few dahlia cultivars. Acta Sci. Pol. Hortorum Cultus 14(6), 189–200.
• Ren, G.H., Chen, J., Zhang, F.F., Yang, H.Y., Zhang, Y.L., Xu, B.L., Zhao, G.Y. (2012). On the technology of tissue culture and rapid reproduction of Dahlia pinnata [J]. J. Beihua Univ. (Nat. Sci.), 4. Available: http://en.cnki. com.cn/Journal_en/A-A000-ZLYY-2012-04.htm
• Salman, M.A., Hamad, M.S., Al-Ahmer, S.M. (2010). In vitro propagation of Dahlia variabilis. Al-Anbar J. Agric. Sci., 8(1), 148–161.
• Sediva, J., Novak, P., Kanka, J., Laxa, J. (2004) Micropropagation, detection and elimination of DMV in the Czech collection of Dahlia. Acta Hortic., 725, 495–498.
• Sharma, H.P., Sinha, A.K., Nag, K.K. (2001). Callus induction and regeneration from shoot apex of Dahlia pinnata var. White alvas. In: Perspectives in biotechnology Procedings of national symposium, Warangal India, 26–27 February 1999. Scientific Publishers, Jodhpur, 121–124.
• Shatilo, V.I. (1991). Some patterns of rhizogenesis in shoots of dahlia in vitro. Byull. Glavn. Bot. Sada, 159, 72–75.
• Shatilo, V.I., Keldysh, M.A., Morozova, S.E. (1991). Hormonal induction of organogenesis in culturing isolated apices of Dahlia. Byull. Glavn. Bot. Sada, 159, 68−72.
• Shivayogeppa, G., Dinakar Adiga, J., Prabhuling, G., Reddy, B.S., Sathyanarayana, B.N. (2008). Strategies for in vitro conservation of Dahlia (Dahlia variabilis L.). Acta Hortic., 865, 393–396.
• Wadankar, G.D., Malode, S.N. (2012). In vitro regeneration of Dahlia cav. J. Global Biosci., 1, 28–41.
• Wang, W.C., Tronchet, M., Larroque, N., Dorion, N., Albouy, J. (1988). Production of virus-free Dahlia by meristem culture and virus detection through cDNA probes and Elisa. Acta Hortic., 234, 421–428.
• Wei, S.L., Li, P.M., He, Z.P., Han, B.W. (1990). Rapid propagation of monomerous stem by tissue culture of Dahlia pinnata Cav. Acta Agr Univ. Pekin., 16(4), 381–385.

Identyfikatory

DOI

10.24326/asphc.2019.5.5