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2013 | 62 | 4 |

Tytuł artykułu

Fast identification of Yersinia pestis, Bacillus anthracis and Francisella tularensis based on conventional PCR

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Rapid and accurate diagnostic tools for detection and identification of Y. pestis, B. anthracis and F. tularensis are essential for timely initial appropriate treatment of exposed individuals, which will be critical to their survival, as well as for reduction of the public health impact and the spread of the disease. The paper presents application of fast polymerases and fast dry electrophoresis in conventional PCR as an alternative for real-time PCR application for detection and identification of the above pathogens. The proposed method takes less than 50 min. to obtain final results of the tests and is cheaper than real-time PCR.

Wydawca

-

Rocznik

Tom

62

Numer

4

Opis fizyczny

p.453-455,fig.,ref.

Twórcy

autor
  • Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland
autor
  • Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland
  • Department of Bacteriology, National Institute of Public Health - National Institute of Hygiene, Chocimska 24, 00-791 Warsaw, Poland

Bibliografia

  • Barns S.M., C.C. Grow, R.T. Okinaka, P. Keim and CR. Kuske. 2005. Detection of diverse new Francisella-like bacteria in environmental samples. Appl. Environ. Microbiol. 71: 5494-5500.
  • Bell CA., J.R. Uhl, T.L. Hadfield, J.C. David, R.F. Meyer, T.F. Smith and F.R. Cockerill III. 2002. Detection of Bacillus anthracis DNA by LightCycler PCR. J. Clin. Microbiol. 40: 2897-2902.
  • Derzelle S., C. Mendy, S. Laroche and N. Madani. 2011. Use of high-resolution melting and melting temperature-shift assay for specific detection and identification of Bacillus anthracis based on single nucleotide discrimination. J. Microbiol. Methods 87: 195-201.
  • Gasper P.W. and R.P. Watson. 2001. Plague and yersiniosis, pp. 313-329. In: Williams E.S. and I.K. Barker (eds). Infectious diseases of wild mammals. 3rd ed. Iowa State University Press, Ames, Iowa.
  • Jackson P.J., M.E. Hugh-Jones, D.M. Adair, G. Green, K.K. Hill, CR. Kuske, L.M. Grinberg, F.A. Abramova and P. Keim. 1998. PCR analysis of tissue samples from the 1979 Sverdlovsk anthrax victims: The presence of multiple Bacillus anthracis strains in different victims. PNAS 95: 1224-1229.
  • Johansson A., A. Ibrahim, I. Goransson, U. Eriksson, D. Gurycova, J.E. Clarridge III and A. Sjostedt. 2000. Evaluation of PCR-based methods for discrimination of Francisella species and subspecies and development of a specific PCR that distinguishes the two major subspecies of Francisella tularensis. J. Clin. Microbiol. 38:4180-4185.
  • Matero P., H. Hemmila, H. Tomaso, H. Piiparinen, K. Rantakokko-Jalava, L. Nuotio and S. Nikkari. 2011. Rapid field detection assay for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis. Clin. Microbiol. Infect. 17: 34-43.
  • Riehm J.M., L. Rahalison, H.C Schulz, B. Thoma, M. Pfeffer, L.M. Razanakoto, S. AI Dahouk, H. Neubauer and H. Tomaso. 2011. Detection of Yersinia pestis using real-time PCR in patients with suspected bubonic plague. Mol. Cell. Probes 25: 8-12.
  • Selvapandiyan A., A. Stabler, N.A. Ansari, S. Kerby, J. Riemenschneider, P. Salotra, R. Duncan and H.L. Nakhasi. 2005. A novel semiquantitative fluorescence-based multiplex polymerase chain reaction assay for rapid simultaneous detection of bacterial and parasitic pathogens from blood. J. Mol. Diagn. 7: 489-494.
  • Tomaso H., H.C. Scholz, H. Neubauer, S. Al Dahouk, E. Seibold, O. Landt, M. Forsman and W.D. Splettstoesser. 2007. Real-time PCR using hybridization probes for the rapid and specific identification of Francisella tularensis subspecies tularensis. Mol. Cell. Probes 21: 12-16.
  • Vogler A.J., D. Birdsell, L.B. Price, J.R. Bowers, S.M. Beckstrom-Sternberg, R.K. Auerbach, J.S. Beckstrom-Sternberg, A. Johansson, A. Clare, J.L. Buchhagen, J.M. Petersen, T. Pearson, J. Vais-saire, J.P. Dempsey, P. Foxall, D.M. Engelthaler, D.M. Wagner and P. Keim. 2009. Phylogeography of Francisella tularensis: global expansion of a highly fit clone. J. Bacteriol. 191: 2474-2484.
  • Zhou D., Y. Han, E. Dai, D. Pei, Y. Song, J. Zhai, Z. Du, J. Wang, Z. Guo and R. Yang. 2004. Identification of signature genes for rapid and specific characterization of Yersinia pestis. Microbiol. Immunol. 48: 263-269.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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