Effect of dialysis of dog semen on sperm characteristics and some biochemical components of seminal plasma

• Autorzy: Strzezek R. , Koziorowska-Gilun M. , Kielczewski K. , Kordan W.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to investigate the effect of dog semen dialysis on sperm characteristics and some biochemical components of seminal plasma. Whole ejaculates were dialyzed against Triscitrate-fructose extender for a 5 h period at room temperature (using semi-permeable cellulose tubing of 12-14 kDa molecular weight cut-off). It has been demonstrated that the long-term dialysis of dog semen causes a significant decrease in sperm quality parameters and disrupts the biochemical properties of seminal plasma. This procedure requires further improvement.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2015
• Tom: 18
• Numer: 2
• Opis fizyczny: p.447-448,ref.

Twórcy

autor

Strzezek R.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Koziorowska-Gilun M.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Kielczewski K.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

autor

Kordan W.

• Department of Animal Biochemistry and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-718 Olsztyn-Kortowo, Poland

Bibliografia

• Buxbaum E (2011) Biophysical chemistry of proteins: An introduction to laboratory methods. 1st ed., Springer, New York.
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• Lampugnani L, Maccheroni M (1984) Rapid colorimetry of zinc in seminal fluid. Clin Chem 30: 1366-1368.
• Mogielnicka-Brzozowska M, Fraser L, Czarzasta J, Kordan W (2012) Isolation and characterization of zinc-binding proteins of canine seminal plasma. Pol J Vet Sci 15: 493-498.
• Strzeżek R, Fraser L (2009) Characteristics of spermatozoa of whole ejaculate and spermrich fraction of dog semen following exposure to media varying in osmolality. Reprod Biol 9: 113-126.
• Strzeżek R, Koziorowska-Gilun M, Kowalówka M, Strzeżek J (2009) Characteristics of antioxidant system in dog semen. Pol J Vet Sci 12: 55-60.

Identyfikatory

DOI

10.1515/pjvs-2015-0057