PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2016 | 85 | 2 |
Tytuł artykułu

Identification of Ephedra species by phylogenetic analyses using matK and ITS1 sequences

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this study, the species identifications of seven Ephedra plants, including three medicinal plants from the Pharmacopoeia of the People’s Republic of China, were conducted using phylogenetic analyses, and the method’s validity was verified. The phylogenetic trees constructed from the maturase-coding gene (matK) and internal transcribed spacer 1 (ITS1) sequences showed that the former could be used for identifying five Ephedra plants, Ephedra intermedia, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. przewalskii, while the latter could distinguish five Ephedra plants, E. przewalskii, E. equisetina, E. antisyphilitica, E. major, and E. aphylla, but it had less power to discriminate E. sinica and E. intermedia. However, when the two genes were combined, the seven species could be completely distinguished from each other, especially the medicinal plants from the others, which is significant in developing their pharmaceutical uses and in performing quality control assessments of herbal medicines. The method presented here could be applied to the analysis of processed Ephedra plants and to the identification of the botanical origins of crude drugs. Additionally, we discovered that E. equisetina and E. major were probably closely related to each other, and that E. sinica, E. intermedia, and E. przewalskii also had a close genetic relationship.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
85
Numer
2
Opis fizyczny
Article 3497 [9p.], fig.,ref.
Twórcy
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
  • Ministry of Education Key Laboratory of Modern Hui Chinese Medicine, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
  • Ministry of Education Key Laboratory of Modern Hui Chinese Medicine, Yinchuan 750004, Ningxia, China
autor
  • Ningxia Medical University Pharmacy College, Yinchuan 750004, Ningxia, China
  • Ningxia Research Center of Modern Hui Medicine Engineering and Technology, Yinchuan 750004, Ningxia, China
Bibliografia
  • 1. Hollander JL, Vander Wall SB, Baguley JG. Evolution of seed dispersal in North American Ephedra. Evol Ecol. 2010;24:333–345. http://dx.doi.org/10.1007/s10682-009-9309-1
  • 2. Flora of China [Internet]. Ephedra przewalskii, Ephedra intermedia. 1999 [cited 2016 Jun 3]. Available from: http://foc.eflora.cn/volume.aspx?num=4
  • 3. Chinese Pharmacopoeia Committee of People’s Republic of China, editor. The Pharmacopoeia of the People’s Republic of China: I. Beijing: Chemical Industry Press; 2015.
  • 4. Zheng ZH, Dong ZH, Yu J. In modern study of traditional Chinese medicine. Beijing: University of Traditional Chinese Medicine; 1997.
  • 5. Asahina H, Shinozaki J, Masuda K, Morimitsu Y, Satake M. Identification of medicinal Dendrobium species by phylogenetic analyses using matK and rbcL sequences. J Nat Med. 2010;64:133–138. http://dx.doi.org/10.1007/s11418-009-0379-8
  • 6. Hilu KW, Liang HP. The matK gene: sequence variation and application in plant systematics. Am J Bot. 1997;84:830–839. http://dx.doi.org/10.2307/2445819
  • 7. Kress WJ, Erickson DL. A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region. PLoS One. 2007;2:e508. http://dx.doi.org/10.1371/journal.pone.0000508
  • 8. Wilson CA. Phylogeny of Iris based on chloroplast matK gene and trnK intron sequence data. Mol Phylogenet Evol. 2004;33:402–412. http://dx.doi.org/10.1016/j.ympev.2004.06.013
  • 9. Newmaster SG, Ragupathy S. Testing plant barcoding in a sister species complex of pantropical Acacia (Mimosoideae, Fabaceae). Mol Ecol Resour. 2009;9:172–180. http://dx.doi.org/10.1111/j.1755-0998.2009.02642.x
  • 10. Guo Y, Tsuruga A, Yamaguchi S, Oba K, Iwai K, Sekita S, et al. Sequence analysis of chloroplast chlB gene of medicinal Ephedra species and its application to authentication of Ephedra herb. Biol Pharm Bull. 2006;29(6):1207–1211. http://dx.doi.org/10.1248/bpb.29.1207
  • 11. Lahaye R, Bank M, Bogarin D, Warner J, Pupulin F, Gigot G, et al. DNA barcoding the floras of biodiversity hotspots. Proc Natl Acad Sci USA. 2008;105:2923–2928. http://dx.doi.org/10.1073/pnas.0709936105
  • 12. Bafeel SO, Arif IA, Bakir MA, Al Homaidan AA, Al Farhan AH, Khan HA. DNA barcoding of arid wild plants using rbcL gene sequences. Genet Mol Res. 2012;11:1934–1941. http://dx.doi.org/10.4238/2012.July.19.12
  • 13. Hou DY, Song JY, Yao H, Han JP, Pang XH, Shi LC, et al. Molecular identification of Corni Fructus and its adulterants by ITS/ITS2 sequences. Chin J Nat Med. 2013;11(2):121–127. http://dx.doi.org/10.3724/SP.J.1009.2013.00121
  • 14. Pang XH, Song JY, Xu HB, Yao H. Identification of Chinese Ephedra herb by ITS2 barcode. Zhong Guo Zhong Yao Za Zhi. 2012;37(8):1118–1120.
  • 15.Huang JL, Giannasi DE, Price RA. Phylogenetic relationships in Ephedra (Ephedraceae) inferred from chloroplast and nuclear DNA sequences. Mol Phylogenet Evol. 2005;35:48–59. http://dx.doi.org/10.1016/j.ympev.2004.12.020
  • 16.Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 1987;19:11–15.
  • 17. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;24:4876–4882. http://dx.doi.org/10.1093/nar/25.24.4876
  • 18. Tamura K, Dudley J, Nei M, Kumar S. MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol. 2007;24:1596–1599. http://dx.doi.org/10.1093/molbev/msm092
  • 19. Huelsenbeck JP, Ronquist F. Mrbayes: Bayesian inference of phylogeny. Bioinformatics. 2001;17:754–755. http://dx.doi.org/10.1093/bioinformatics/17.8.754
  • 20. Nylander JAA. MrModeltest, version 1.0b [Internet]. 2016 [cited 2016 May 25]. Available from: https://www.abc.se/~nylander/
  • 21. Swofford DL. PAUP*: phylogenetic analysis using parsimony (and other methods), version 4.0b10. Sunderland, MA: Sinauer; 2003.
  • 22. Wang QB, Wang L, Zhou RC, Zhao XM, Shi SH, Yang Y, et al. Phylogenetic position of Ephedra rhytidosperma, a species endemic to China: evidence from chloroplast and ribosomal DNA sequences. Chin Sci Bull. 2005;50:2901–2904. http://dx.doi.org/10.1007/BF02899657
  • 23. Long CF, Kakiuchi N, Takahashi A, Komatsu K, Cai SQ, Mikage M. Phylogenetic analysis of the DNA sequence of the non-coding region of nuclear ribosomal DNA and chloroplast of Ephedra plants in China. Planta Med. 2004;70:1080–1084. http://dx.doi.org/10.1055/s-2004-832651
  • 24. Long C, Kakiuchi N, Zhong G, Mikage M. Survey on resources of Ephedra plants in Xinjiang. Biol Pharm Bull. 2005;28:285–288. http://dx.doi.org/10.1248/bpb.28.285
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d51a7746-74cc-44f6-9a49-3d42cb44a09c
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.