Development of real-time PCR assay for detection of vibrio cholerae

• Autorzy: Bielawska-Drozd A. , Mirski T. , Bartoszcze M. , Cieslik P. , Roszkowiak A. , Michalski A.
• Języki publikacji: EN

Abstrakty

EN

Vibrio cholerae is the etiological agent of epidemic cholera. Symptoms include stomach ache, bloody diarrhea, and vomiting, which lead to severe dehydration and even death. Environmental monitoring as well as rapid and accurate identification of this pathogen are important for public health protection. In this study, a real-time PCR method for the detection of toxigenic V. cholerae was developed. In total, 63 environmental and clinical strains were tested for the presence of seven targets, namely ompW, ompU, tcpA, ctxA, zot, rfbO1, and rfbO139. The proposed method is specific, simple, and fast, and can be used for detection of toxigenic and non-toxigenic V. cholerae strains. The minimum detection limit of this assay for V. cholerae in environmental water samples was 1.4 CFU/ml.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2012
• Tom: 21
• Numer: 2
• Opis fizyczny: p.279-288,fig.,ref.

Twórcy

autor

Bielawska-Drozd A.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

autor

Mirski T.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

autor

Bartoszcze M.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

autor

Cieslik P.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

autor

Roszkowiak A.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

autor

Michalski A.

• Biological Threat Identification and Countermeasure Centre of the Military Institute of Hygiene and Epidemiology, 2 Lubelska, 24-100 Puławy, Poland

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