PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 18 | 4 |
Tytuł artykułu

Prevalence and risk factors for Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in finishing pigs in Polish farrow - to - finish swine herds

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to estimate the herd-level, within-herd prevalence, the frequency of mixed infections and risk factors for L. intracellularis, B. hyodysenteriae and Salmonella spp. in selected farrow-to-finish Polish pig herds. A total of 254 pooled fecal samples were collected from 9 to 24 week-old pigs in 70 herds. Real time PCR for detection of L. intracellularis and B. hyodysenteriae was performed. For Salmonella spp. bacteriological examination was performed. The herd-level prevalences of L. intracellularis, B. hyodysenteriae and Salmonella spp. among examined herds were 65.7%, 1.4% and 8.6%, respectively. The within-herd prevalences (in positive herds) for L. Intracellularis, B. hyodysenteriae and Salmonella spp. were 51.5%, 75.0% and 30.4%, respectively. All herds with diarrhea observed during sampling were infected with L. intracellularis and 60% of herds with no diarrhea at the moment of sampling were infected with L. intracellularis (p=0.035). In herds with more than 200 sows the prevalence of Salmonella spp. was significantly higher compared to herds with less than 200 sows (p=0.027). In herds where all-in/all-out (AIAO) was respected, prevalence of L. intracellularis was significantly lower than in herds where this rule was not kept (p=0.024). Obtained results confirm that L. intracellularis is the major cause of bacterial diarrhea in finishing pigs. The present study identified AIAO and herd size as a risk factor, at the herd level, for L. Intracellularis and Salmonella spp., respectively.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
4
Opis fizyczny
p.825-831,fig.,ref.
Twórcy
autor
  • Department of Swine Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
  • Department of Swine Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Swine Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Swine Diseases, National Veterinary Research Institute, Partyzantow 57, 24-100 Pulawy, Poland
Bibliografia
  • Arguello H, Sorensen G, Carvajal A, Baggesen D, Rubio P, Pedersen K (2013) Prevalence, serotypes and resistance patterns of Salmonella in Danish pig production. Res Vet Sci 95: 334-342.
  • Biksi I, Lorincz M, Molnár B, Kecskés T, Takács N, Mirt D, Cizek A, Pejsak Z, Martineau GP, Sevin JL, Szenci O (2007) Prevalence of selected enteropathogenic bacteria in Hungarian finishing pigs. Acta Vet Hung 55: 219-227.
  • Bonardi S, Bassi L, Brindani F, D’Incau M, Barco L, Carra E, Pongolini S (2013) Prevalence, characterization and antimicrobial susceptibility of Salmonella enterica and Yersinia enterocolitica in pigs at slaughter in Italy. Int J Food Microbiol 163: 248-257.
  • Carlson S, Branhill A, Griffith R (2012) Salmonellosis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW (eds) Diseases of Swine, Wiley-Blackwell, Ames, Iowa, pp 821-833.
  • Cizek A, Sperling D, Bednar D, Pejsak Z, Biksi I, Martineau GP, Sevin JL, Hasman P, Mirt D (2006) Lawsonia intracellularis and Brachyspira spp. prevalence in fatteners in the Czech Republic, Hungary and Poland Czech large-scale farms without diarrhea. Proceedings of the 19th IPVS Congress, Copenhagen, Denmark, 2: 356.
  • Clopper CJ, Pearson ES (1934). The use of confidence or fiducial limits illustrated in the case of the binomial. Biometrika 26: 404-413.
  • Correia-Gomes C, Mendonc¸a D, Vieira-Pinto M, Niza-Ribeiro J (2013) Risk factors for Salmonella spp. in Portuguese breeding pigs using a multilevel analysis. Prev Vet Med 108: 159-166.
  • EFSA (2009) Analysis of the baseline survey on the prevalence of Salmonella in holdings with breeding pigs in the EU, 2008, Part A: Salmonella prevalence estimates, EFSA Journal 7(12): 1377 [p 93].
  • Fellström C, Pettersson B, Thomson J, Gunnarsson A, Persson M, Johansson KE (1997) Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR. J Clin Microbiol 35: 462-467.
  • Grimont PAD, Weill F-X (2007) Antigenic formulae of the Salmonella serovars https://www.pasteur.fr/sante/clre/cadrecnr/salmoms/WKLM-En.pdf
  • Hampson DJ (2012) Brachyspiral Colitis. In: Zimmerman JJ, Karriker LA, Ramirez A, Schwartz KJ, Stevenson GW (eds) Diseases of Swine, Wiley-Blackwell, Ames, Iowa, pp 680-696.
  • Hands I, McOrist S, Blunt R, Lawrence K (2010) Current infection patterns of porcine proliferative enteropathy in Great Britain and the Republic of Ireland. Vet Rec 167: 343-344.
  • Jacobson M, Gerth Löfstedt M, Holmgren N, Lundeheim N, Fellström C (2005) The prevalences of Brachyspira spp. and Lawsonia intracellularis in Swedish piglet producing herds and wild boar population. J Vet Med B Infect Dis Vet Public Health 52: 386-391.
  • Jensen TK, Møller K, Leser TD, Jorsal SE (1997) Comparison of histology, immunohistochemistry and polymerase chain reaction for detection of Lawsonia intracellularis in natural porcine proliferative enteropathy. Eur J Vet Pathol 3: 115-123.
  • Jørgensen F, Bailey R, Williams S, Henderson P, Wareing DR, Bolton FJ, Frost JA, Ward L, Humphrey TJ (2002) Prevalence and numbers of Salmonella and Campylobacter spp. on raw, whole chickens in relation to sampling methods. Int J Food Microbiol 76: 151-164.
  • Laanen M, Persoons D, Ribbens S, de Jong E, Callens B, Strubbe M, Maes D, Dewulf J (2013) Relationship between biosecurity and production/antimicrobial treatment characteristics in pig herds. Vet J 198: 508-512.
  • Lawson GH, Gebhart CJ (2000) Proliferative enteropathy. J Comp Pathol 122: 77-100.
  • Lee SW, Kim TJ, Park SY, Song CS, Chang HK, Yeh JK, Park HI, Lee JB (2001) Prevalence of porcine proliferative enteropathy and its control with tylosin in Korea. J Vet Sci 2: 209-212.
  • Li R, Lai J, Wang Y, Liu S, Li Y, Liu K, Shen J, Wu C (2013) Prevalence and characterization of Salmonella species isolated from pigs, ducks and chickens in Sichuan Province, China. Int J Food Microbiol 163: 14-18.
  • McOrist S (2005) Defining the full costs of endemic porcine proliferative enteropathy. Vet J 170: 8-9.
  • Merialdi G, Bonilauri P, Granelli F, Luppi A, Dottori M (2003) Bacterial pathogens in field cases of clinical colitis in growing and finishing pigs in Italy. Vet Rec 153: 529-530.
  • Nathues C, Grüning P, Fruth A, Verspohl J, Blaha T, Kreienbrock L, Merle R (2013) Campylobacter spp., Yersinia enterocolitica, and Salmonella enterica and their simultaneous occurrence in German fattening pig herds and their environment. J Food Prot 76: 1704-1711.
  • Pedersen KS, Ståhl M, Guedes RM, Angen Ø, Nielsen JP, Jensen TK (2012) Association between faecal load of Lawsonia intracellularis and pathological findings of proliferative enteropathy in pigs with diarrhoea. BMC Vet Res 8: 198.
  • Pejsak Z, Żmudzki J, Wasak M, Porowski M, Wojciechowski J, Mirt D (2007) Prevalence of Brachyspira hyodysenteriae and Lawsonia intracellularis infections in Polish swine population. Med Weter 63: 994-996.
  • Pejsak Z, Żmudzki J, Wojciechowski J, Salwa A (2009) Prevalence and economic costs of proliferative enteropathy in the Polish swine herd population. Med Weter 65: 258-261.
  • Reiner G, Hillen S, von Berg S, Kixmoller M, Willems H (2011) Analysis of bacterial load and prevalence of mixed infections with Lawsonia intracellularis, Brachyspira hyodysenteriae and/or Brachyspira pilosicoli in German pigs with diarrhoea. Berl Munch Tierarztl Wochenschr 124: 236-241.
  • Soper DS (2014) Binomial Probability Confidence Interval Calculator. Available from http://www.danielsoper.com/statcalc
  • Stege H, Jensen TK, Møller K, Baekbo P, Jorsal SE (2000) Prevalence of intestinal pathogens in Danish finishing pig herds. Prev Vet Med 46: 279-292.
  • Suh DK, Song JC (2005) Prevalence of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella in swine herds. J Vet Sci 6: 289-293.
  • Vico JP, Rol I, Garrido V, San Román B, Grilló MJ, Mainar-Jaime RC (2011) Salmonellosis in finishing pigs in Spain: prevalence, antimicrobial agent susceptibilities, and risk factor analysis. J Food Prot 74: 1070-1078.
  • Viott AM, Lage AP, Cruz EC Jr, Guedes RM (2013) The prevalence of swine enteropathogens in Brazilian grower and finish herds. Braz J Microbiol 44: 145-151.
  • Wasyl D, Hoszowski A (2007) Occurrence of Salmonella in slaughter pigs in Poland. PIWet-PIB, Puławy, pp 1-26.
  • Zmudzki J, Szczotka A, Podgórska K, Nowak A, Grzesiak A, Dors A, Pejsak Z (2012) Application of real-time PCR for detection of Lawsonia intracellularis and Brachyspira hyodysenteriae in fecal samples from pigs. Pol J Vet Sci 15: 267-273.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d3ffff16-cbb5-4b97-bd12-e3a8e8d51442
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.