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2014 | 70 | 10 |

Tytuł artykułu

Izolacja szczepów Salmonella Typhimurium var. Copenhagen od padłych gołębi i ocena ich immunogenności

Warianty tytułu

EN
Isolation of Salmonella Typhimurium var. Copenhagen strains from dead pigeons and evaluation of their immunogenicity

Języki publikacji

PL

Abstrakty

EN
The aim of the study was to isolate and identify the pathogen responsible for mortality in pigeons, and to choose the most immunogenic strain of Salmonella to prepare a vaccine. The study was conducted in two stages. The first stage consisted of isolating and identifying the strains, as well as preparing vaccine prototypes. The material for bacteriological examination comprised samples of parenchymal organs (heart, liver, spleen), intestines, and swabs from synovial cavities. Three strains were isolated and identified as Salmonella Typhimurium var. Copenhagen by biochemical and serological tests. The isolates were used to prepare three prototype vaccines, which were combined with an adjuvant consisting of Carbomer and Ginseng extract. In the second stage, the immunogenicity of the prototype vaccines was evaluated in 24 racing pigeons aged 6-8 weeks, which were divided into 4 experimental groups. Agglutination antibody titers in the serum of immunized birds were evaluated by the tube agglutination test at days 0, 7, 14, and 28 after vaccination. One of the three experimental vaccines generated higher agglutination titers than the others, and was selected for further testing.

Wydawca

-

Rocznik

Tom

70

Numer

10

Opis fizyczny

s.620-625,tab.,bibliogr.

Twórcy

  • Zakład Mikrobiologii Weterynaryjnej, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-950 Lublin
autor
  • Laboratorios Hipra S.A., Av. La Selva, 13, 17170 Amer (Girona), Hiszpania
  • AVI EXPERT SPWCP, ul.Gajowa 1, 20-827 Lublin
autor
  • VEPHARM, ul. Karpińskiego 7, 24-100 Puławy

Bibliografia

  • 1. Abdolvahab F., Friendship R. M.: A clinical field trial to evaluate the efficacy of vaccination in controlling Salmonella infection and the association of Salmonella-shedding and weight gain in pig. Can. J. Vet. Res. 2010, 74, 258-263.
  • 2. Boś M.: Wpływ procesu nabywania antybiotykooporności na wybrane cechy fenotypowe i genotypowe pałeczek z rodzaju Salmonella w badaniach in vitro. Praca doktorska, Akademia Rolnicza, Lublin 2000.
  • 3. Dorn Ch., Schroeter A., Helmuth R.: Salmonella isolates typed in national reference laboratory in Germany in years 1998-2001. International Symposium „Salmonella and Salmonellosis”, Ploufragran, France 2002, s. 603-604.
  • 4. Duchatel J. P., Vindevogel H.: Assessment of vaccination of pigeons against paramyxovirus type 1 infection with inactivated aqueous- suspension or oil-emulsion vaccines. Avian Pathol. 1986, 15, 455-465.
  • 5. Duchatel J. P., Vindevogel H.: Premiers essais de vaccination de pigeons contre la paratyphose au moyen de vaccins inactives adjuves aqueux. Ann. Med. Vet. 1996, 140, 161-163.
  • 6. Faddoul G. P., Fellows G. W.: Clinical Manifestations of Paratyphoid Infection in Pigeons. Avian Dis. 1965, 22, 377-381.
  • 7. French G., Schwarz S.: Genotyping and identification of the resistance genes of multiresistant Salmonella enteritice supsp. Enteric serovar Typhimurium var. Copenhagen isolates from different animal sources. Mat z konf. Salmonella and Salmonellosis, 2002, I3S, 483-488.
  • 8. Jordan R., van Heerden E., Hugo C. J., Piater L. A.: Using current molecular techniques for rapid differentiation of Salmonella Typhi and Salmonella Typhimurium. Afr. J. Biotechnol. 2009, 8, 1815-1818.
  • 9. Leekitcharoenphon P., Lukjancenko O., Friis C., Frank M., Aarestrup F. M., Ussery D.: Genomic variation in Salmonella Enterica core genes for epidemiological typing. BMC Genomics 2012, 13, 88.
  • 10. Li Y., Yan X., Hu S.: Ginseg Stem-Leaf Saponins and Oil Adjuavant Synergistricaly promote the immune responses to Newcastle Disease in chickens. J. Animal Vet. Adv. 2012, 11, 2423-2428.
  • 11. Lukjancenko O, Wassenaar T. M., Ussery D. W.: Comaparison of 61 sequenced Escherichia Coli Genomes. Microbiol. Ecol. 2010, 60, 708-720.
  • 12. Mi-Hyoung Kim, Yun-Young Byon, Eun-Ju Ko, Jie-Young Song, Yeon-Sook Yun, Taekyun Shin, Hong-Gu Joo.: Immunomodulatory Activity of Ginsan, a Polysaccharide of Panax Ginseg, on Dendritic Cells. Korean J. Physiol. Pharmacol. 2009, 13, 169-175.
  • 13. Mumford J. A., Wilson H., Hannant D., Jessett D. M.: Antigenicity and immunogenicity of equine influenza vaccines containing a Carbomer adjuvant. Epidemiol. Infect. 1994, 112, 421-437.
  • 14. Na H. S., Lim Y. J., Yun Y. S., Kweon M. N., Lee H. C.: “Ginsan enhances humoral antibody response to orally delivered antigen”. Immune Network. 2010, 10, 5-14.
  • 15. Pasmans F., Baert K., Martel A., Bousquet-Melou A., Lanckriet R., De Boever S., Van Immerseel F., Eeckhaut V., de Backer P., Haesebrouck F.: Induction of the Carrier State in Pigeons Infected with Salmonella enteric subspecies enterica Serovar Typhimurium PT99 by Treatment with Florfenicol: a Matter of Pharmacokinetics. Antimicrobial Agents and Chemotherapy 2008, 52, 954-961.
  • 16. Pasmans F., Van Immerseel F., Hermans K., Heyndrickx M., Collard J.-M., Ducatelle R., Haesebrouck R., Haesebrouck F. L.: Assessment of Virulence of Pigeon Isolates of Salmonella enterica subsp. enterica Serovar Typhimurium Variant Copenhagen for Humans. J. Clin. Microbiol. 2004, 42, 2000-2002.
  • 17. Pasmans F., Van Immerseel F., Heyndrickx M., Martel A., Godard C., Wildemauwe C., Ducatelle R., Haesebrouck F.: Host adaptation of pigeon isolates of Salmonella enterica subsp. enterica serovar Typhimurium variant Copenhagen phage type 99 is associated with enhanced macrophage cytotoxicity. Infect. Immunol. 2003, 71, 6068-6074.
  • 18. Pedersen K., Clark L., Andelt W. F., Salman M. D.: Prevalence of shiga toxin producing Escherichia coli and Salmonella enterica in rock pigeons captured in Fort Collins, Colorado. J. Wild. Dis. 2006, 42, 46-55.
  • 19. Polski Komitet Normalizacyjny, Mikrobiologia żywności i pasz, Horyzontalna metoda wykrywania Salmonella spp., zmiana do Polskiej Normy, dotyczy PN-EN ISO 6579: 2003, Mikrobiologia żywności i pasz, Horyzontalna metoda wykrywania Salmonella spp. PN-EN ISO 6572:2003/A1, grudzień 2007.
  • 20. Rabsch W., Tschaepe H., Baeumler A. J.: Host adaptation of Salmonella enterica serovar Typhimurium variants. Reports and Communications, Salmonella and Salmonellosis, Saint-Brieuc, France 2002, I3S, 483-488.
  • 21. Rivera E., Ekholm Pettersson F., Inganäs M., Paulie S., Gronvik K. O.: The Rb1 fraction of ginseng elicits a balanced Th1 and Th2 immune response. Vaccine 2005, 23, 5411-5419.
  • 22. Selvaraj P., Pitchappan R. M.: Post-hatching development of the Immune System of Pigeon, Columba Livia. Devel. Comp. Immunol. 1988, 12, 879-884.
  • 23. Skov M. N., Sandvang D., Torpdahl M., Baggesen D. L.: Investigation of sources of human Salmonella Typhimurium infections in Denmark- using pulset field gel electrophoresis and plazmid analysis. Reports and Communications, Salmonella and Salmonellosis, Saint-Brieuc, France 2002, 55-61.
  • 24. Skowron M.: Wpływ antybiotykoterapii na wartość wybranych metod stosowanych w diagnostyce zakażeń pałeczkami z rodzaju Salmonella. Praca doktorska, Akademia Rolnicza, Lublin 2005.
  • 25. Szeleszczuk P., Kosowska G.: Salmonelloza. Wciąż najważniejszy problem w patologii gołębi. Weterynaria w terenie 2007, 2, 47-52.
  • 26. Tanaka C., Miyazawa T., Watarai M., Ishiguro N.: Bacteriological survey of feces from feral pigeons in Japan. J. Vet. Med. Sci. 2005, 67, 951-953.
  • 27. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J.: 16S ribosomal DNA amplification for phylogenetic study. J. Bacteriol. 1991, 173 (2), 697-703.
  • 28. Zhai L., Li Y., Wang W., Wang Y., Hu S.: Effect of oral administration of ginseng stem-and-leaf saponins (GSLS) on the immune responses to Newcastle disease vaccine in chickens. Vaccine 2011, 29, 5007-5014.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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