The optimization of extraction process of white mulberry leaves and the characteristic bioactive properties its powder extract

• Autorzy: Przygonski K. , Wojtowicz E.
• Języki publikacji: EN

Abstrakty

EN

ntroduction: Powder extracts from white mulberry leaves (Morus alba L.) are desirable due to their simplicity of use. Powders intended for use in food and dietary supplements can be obtained by various methods, including water extraction and spray drying. Objective: The aim of the work was to optimize the aqueous process of extraction of white mulberry leaves and characterization of bioactive properties of the obtained extract powder. Methods: The DNJ and flavonoids (quercetin, kaempferol) contents and were analysed by high performance liquid chromatography (HPLC). Total phenolic contents were determined using the Folin-Ciocalteu phenol reagent and antioxidant activity by ABTS and DPPH assays. Results: The mulberry leaf extraction process has been optimized. The obtained powdered mulberry leaf extract proved to be a good source of bioactive compounds. Characteristic phenolic compounds detected in mulberry leaves and their powder extract were quercetin and kaempferol. The sum of polyphenols was 10.9 mg GAE/g dry matter in the raw material, whereas in the powder extract 42.6 mg GAE/g dry matter. In addition, it was found that the obtained powdered extract is characterized by a five-fold higher, in comparison to the raw material, the content of antioxidant activity measured by ABTS and DPPH tests. The content of 1-deoxynojirimycin (DNJ) in the powder extract was nearly four times higher than in dry mulberry leaves. Conclusion: The optimized process of water extraction of white mulberry leaves allows to keep valuable bioactive components and to obtain their high concentration.

Słowa kluczowe

EN

extraction; optimization; white mulberry; Morus alba; leaf; bioactive property; powder extract; antioxidant activity; flavonoids; 1-deoxynojirimycin; 1-DNJ zob.1-deoxynojirimycin

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2019
• Tom: 65
• Numer: 1
• Opis fizyczny: p.12-19,fig.,ref.

Twórcy

autor

Przygonski K.

• Department of Food Concentrates and Starch Products, Prof.Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Starolecka 40, 61-361 Poznan, Poland

autor

Wojtowicz E.

• Department of Food Concentrates and Starch Products, Prof.Waclaw Dabrowski Institute of Agricultural and Food Biotechnology, Starolecka 40, 61-361 Poznan, Poland

Bibliografia

• 1. Butt MS, Nazira A, Sultan MT, Schroen K. Morus alba L. nature’s functional tonic. Trends Food Sci Technol 2008; 19:505-512.
• 2. Katsube T, Imawaka N, Kawano Y, Yamazaki Y, Shiwaku K, Yamane Y. Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chem 2006; 97:25-31. doi: http://dx.doi.org/10.1186/1472-6882-14-139
• 3. Katsube T, Yamasaki M, Shiwaku K, Ishijima T, Matsumoto I, Abe K, Yamasaki Y. Effect of flavonol glycoside in mulberry (Morus alba L.) leaf on glucose metabolism and oxidative stress in liver in diet-induced obese mice. J Sci Food Agric 2010; 90:2386-2392. doi: http://dx.doi.org/10.1002/jsfa.4096
• 4. Thabti I, Elfalleh W, Hannachi H, Ferchichi A, Campos MG. Identification and quantification of phenolic acids and flavonol glycosides in Tunisian Morus species by HPLC-DAD and HPLC MS. J Func Foods 2012; 4:367-374. doi: http://dx.doi.org/10.1016/j.jff.2012.01.006
• 5. Halliwell B. Dietary polyphenols: good, bad, or indifferent for your health? Cardiovasc Res 2007; 73: 341-347. doi: http://dx.doi.org/10.1016/j.cardiores.2006.10.004
• 6. Chung HI, Kim J, Kim JY, Kwon O. Acute intake of mulberry leaf aqueous extract affects postprandial glucose response after maltose loading randomized double-blind placebo-controlled pilot study. J Func Foods 2013; 5:1502-1506.
• 7. Jeszka-Skowron M, Flaczyk E, Jeszka J, Krejpcio Z, Król E, Buchowski MS. Mulberry leaf extract intake reduces hyperglycaemia in streptozotocin (STZ)-induced diabetic rats fed high-fat diet. J Func Foods 2014; 8:9-17. doi: http://dx.doi.org/10.15193/zntj/2014/92/148-159
• 8. Liu LK, Chou FP, Chen YC, Chyau CC, Ho HH, Wang CJ. Effects of mulberry (Morus alba L.) extracts on lipid homeostasis in vitro and in vivo. J Agric Food Chem 2009; 57:7605-7611. doi:http://dx.doi.org/10.1021/jf9014697
• 9. Memon A, Memon N, Luthria D, Bhanger M, Pitafi A. Phenolic acids profiling and antioxidant potential of mulberry (Morus laevigata W., Morus nigra L., Morus alba L.) leaves and fruits grown in Pacistan. Pol J Food Nutr 2010, 60:25-32
• 10. Nuengchamnong N, Ingkaninan K, Kaewruang W, Wongareonwanakij S, Hongthongdaeng B. Quantitative determination of 1-deoxynojirimycin in mulberry leaves using liquid chromatography-tandem mass spectrometry. J Pharm Biomed Anal 2007; 44:853-858. doi: http://dx.doi.org/10.1016/j.jpba.2007.03.031
• 11. Vachasilp Ch, Nakagawa K, Sookwong P, Higuchi O, Kimura F. A novel gelatin crosslinking method retards release of mulberry 1-deoxynojirimycin providing a prolonged hypoglycaemic effect. Food Chem 2012; 134:1823-1830. doi: http://dx.doi.org/10.1016/j.foodchem.2012.03.086
• 12. Kim J-W, Kim S-U, Lee HS, Kim I, Ahn MY, Ryu KS. Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversephase high-performance liquid chromatography. J Chromatogr A 2003; 1002:93-99.
• 13. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic – phosphotungstic acid reagents. Am J Enol Vitic 1965; 16:144-158. doi: http://dx.doi.org/10.3390/ijms11062362
• 14. Nuutila AM, Puupponen-Pimiä R, Aarni M, Oksman-Caldentey KM. Comparison of antioxidant activities of onion and garlic extracts by inhibition of lipid peroxidation and radical scavenging activity. Food Chem 2003; 81:485-493. doi: http://dx.doi.org/10.1016/S0308-8146(02)00476-4
• 15. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radi Bio Med 1999; 26: 1231-1237. doi: http.//dx. doi.org /10.1016/S0891-5849(98)00315-3
• 16. Hertog J, Pals CEGM, Jonk LJC, Kruijer W. Differential expression of a novel murine non-receptor protein tyrosine phosphatase during differentiation of P19 embryonal carcinoma cells. Biochem Biophys Res Commun 1992; 184:1241-1249. doi: http://doi.org/10.1016/S0006-291X(05)80015-4
• 17. Sakakibara H, Honda Y, Nakagawa S, Ashida H, Kanazawa K. Simultaneous determination of all polyphenols in vegetables, fruits, and teas. J Agric Food Chem 2003; 51:571-81. doi: http://dx.doi.org/10.1021/jf020926l
• 18. Hu X-Q, Jiang L, Zhang J-G, Deng W, Wang H-L, Wei Z-J. Quantitative determination of 1-deoxynojirimycin in mulberry leaves from 132 varieties. Ind Crop Prod 2013; 49:782–784. doi: http://dx.doi.org/10.1016/j.indcrop.2013.06.030
• 19. Song W, Wang HJ, Bucheli P, Zhang PF, Wei DZ, Lu YH. Phytochemical profiles of different mulberry (Morus sp.) species from China. J Agric Food Chem 2009; 57:9133-9140. doi: http://dx.doi.org/10.1021/jf9022228
• 20. Lee NK, Jeong JH, Oh J, Kim Y, Ha YS, Jeong Y-S. Conversion of flavonols kaempferol and quercetin in mulberry (Morus alba L.) leaf using plant-fermenting lactobacillus plantarum. J Food Biochem 2015; 39: 765-770. doi: http://doi.org/10.1111/jfbc.12176
• 21. Zou Y, Liao S, Shen W, Liu F, Tang C, Chen CHYO, Sun Y. Phenolics and antioxidant activity of mulberry leaves depend on cultivar and harvest month in southern China. Int J Mol Sci 2012; 13:16544-16533. doi: http://dx.doi.org/10.3390/ijms131216544
• 22. Wanyo P, Siriamornpun S, Meeso N. Improvement of quality and antioxidant properties of dried mulberry leaves with combined far-infrared radiation and air convection in Thai tea process. Food Bioprod Process 2011; 89:22-30. doi: http://dx.doi.org/10.1016/j.fbp.2010.03.005
• 23. Tan JJY, Lim YY, Siow LF, Tan JBL. Effects of drying on polyphenol oxidase and antioxidant activity of Morus alba leaves. J Food Process Preserv 2015; 39:2811-2819. doi: http://dx.doi.org/10.1111/jfpp.12532
• 24. Arabshahi-Delouee S, Urooj A. Antioxidant properties of various solvent extracts of mulberry (Morus indica L.) leaves. Food Chem 2007; 102:1233-1240. doi: http://dx.doi.org/10.1016/j.foodchem.2006.07.013
• 25. Sánchez-Salcedo EV, Mena P, Garcia-Viguera C, Hernández F, Martinéz JJ. (Poly)phenolic compounds and antioxidant activity of white (Morus alba) and black (Morus nigra) mulberry leaves: Their potential for new products rich in phytochemicals. J Fun Foods 2015; 18:1039-1046. doi: http://dx.doi.org/10.1515/fhort-2017-0023

Identyfikatory

DOI

10.2478/hepo-2019-0003