Changes in endogenous hormones and oxidative burst as the biochemical basis for enhanced shoot organogenesis in cold-treated Saussurea involucrata explants

• Autorzy: Guo B. , Stiles A. , Liu C.-Z.
• Języki publikacji: EN

Abstrakty

EN

A low temperature treatment significantly improved shoot morphogenesis of Saussurea involucrata Kar. et Kir leaf explants. The biochemical mechanisms underlying the cold-induced shoot organogenesis were investigated by measuring endogenous plant growth hormones, peroxide radicals, and superoxide dismutase (SOD) activity in the cold-treated leaf explants compared to controls. The ratio of zeatin (ZT) to indole-3-acetic acid (IAA) significantly increased in leaf explants subjected to a 5-day treatment at 4 C compared to controls. The accumulation of O2 2 also rapidly increased in response to cold treatment, and then decreased as SOD activity catalyzed the dismutation of O2 2 to molecular oxygen and H2O2; this resulted in a significant increase in H2O2 concentrations in the cold-treated explants. We propose that a combination of the increased ZT/IAA ratio and H2O2 concentration is the basis for the enhanced shoot morphogenesis in response to cold treatment. These results provide a starting point for an improved understanding of the biochemical mechanisms underlying cold-induced shoot organogenesis of this unique plant species.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 01
• Opis fizyczny: p.283-287,fig.,ref.

Twórcy

autor

Guo B.

• National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China

autor

Stiles A.

• National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China

autor

Liu C.-Z.

• National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People’s Republic of China

Bibliografia

• Apel K, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55:373–399
• Beaucham C, Fridovich I (1971) Superoxide dismutase—improved assays and an assay applicable to acrylamide gels. Anal Biochem 44(1):276–287
• Bolduc RJ, Cherry JH, Blair BO (1970) Increase in indoleacetic acid oxidase activity of winter wheat by cold treatment and gibberellic acid. Plant Physiol 45(4):461–464
• Duclercq J, Sangwan-Norreel B, Catterou M, Sangwan RS (2011) De novo shoot organogenesis: from art to science. Trends Plant Sci 16(11):597–606
• Fu LG (1992) China plant red data book—rare and endangered plants, vol vol 1. Chinese Science Press, Beijing
• Guo B, Gao M, Liu CZ (2007) In vitro propagation of an endangered medicinal plant Saussurea involucrata Kar. et Kir. Plant Cell Rep 26(3):261–265
• Guo B, Abbasi BH, Wei YH (2011) Effects of hypobaric growth conditions on morphogenic potential and antioxidative enzyme activities in Saussurea involucrata. Biol Plantarum 55(4): 783–787
• Gupta SD (2011) Role of free radicals and antioxidants in in vitro morphogenesis. In: Reactive oxygen species and antioxidants in higher plants. Science Publishers, USA, pp 229–247
• Gupta SD, Datta S (2003) Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. Biol Plantarum 47(2): 179–183
• Hou BK, Yu HM, Teng SY (1997) Effects of low temperature on induction and differentiation of wheat calluses. Plant Cell Tiss Org 49(1):35–38
• Li GH, Zhao RC (1989) Studies on pharmacological actions of Saussurea involucrata Kar. et Kir. Acta Pharma Sinica 15:368–369
• Liu CZ, Murch SJ, Jain JC, Saxena PK (2004a) Goldenseal (Hydrastis canadensis L.): in vitro regeneration for germplasm conservation and elimination of heavy metal contamination. In Vitro Cell Dev Biol-Plant 40:75–79
• Liu CZ, Murch SJ, El-Demerdash M, Saxena PK (2004b) Artemisia judaica L.: mass propagation and antioxidant potential. J Biotechnol 110:63–71
• Ma ZC, Chu KM (1994) Study on method for analysis of cytokinins by high performance liquid chromatography. J Instrum Anal 13(6):88–91
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7(9):405–410
• Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002) Hydrogen peroxide and nitric oxide as signalling molecules in plants. J Exp Bot 53(372):1237–1247
• Papadakis AK, Roubelakis-Angelakis KA (2002) Oxidative stress could be responsible for the recalcitrance of plant protoplasts. Plant Physiol Biochem 40(6–8):549–559
• Sarul P, Vlahova M, Ivanova A, Atanassov A (1995) Direct shoot formation in spontaneously occurring root pseudonodules of Alfalfa (Medicago-Sativa L). In Vitro Cell Dev-Pl 31(1):21–25
• Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 101(1):7–12
• Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tisssues cultured in vitro. Symp Soc Exp Biol 54:118–130
• Szechynska-Hebda M, Skrzypek E, Dabrowska G, Wedzony M, van Lammeren A (2012) The effect of endogenous hydrogen peroxide induced by cold treatment in the improvement of tissue regeneration efficiency. Acta Physiol Plant 34:547–560
• Tanaka M, Takei K, Kojima M, Sakakibara H, Mori H (2006) Auxin controls local cytokinin biosynthesis in the nodal stem in apical dominance. Plant J 45(6):1028–1036
• Tian M, Gu Q, Zhu MY (2003) The involvement of hydrogen peroxide and antioxidant enzymes in the process of shoot organogenesis of strawberry callus. Plant Sci 165(4):701–707
• Tsugawa H, Suzuki M (2000) A low-temperature method for maintaining plant regeneration activity in embryogenic callus of rice (Oryza sativa L.). Plant Cell Rep 19(4):371–375
• Wang A, Luo G (1990) Quantitative relation between the reaction of hydroxylamine and superoxide anion radicals in plants. Plant Physiol Commun 6:55
• Wu LQ, Lv YL, Meng ZX, Chen J, Guo SX (2010) The promoting role of an isolate of dark-septate fungus on its host plant Saussurea involucrata Kar. et Kir. Mycorrhiza 20(2):127–135
• Yoshimatsu K, Shimomura K (1994) Plant-regeneration on cultured root segments of Cephaelis ipecacuanha A. Richard. Plant Cell Rep 14(2–3):98–101
• Zhang CG, Li W, Mao YF, Zhao DL, Dong W, Guo GQ (2005) Endogenous hormonal levels in Scutellaria baicalensis calli induced by thidiazuron. Russ J Plant Physiol 52(3):345–351

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