Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 29 | 4 |
Tytuł artykułu

The role of oxidative stress induced by growth regulators in the regeneration process of wheat

Warianty tytułu
Języki publikacji
As part of work to optimize the regeneration processes of winter wheat callus culture the effects of two auxins (2,4-D, IAA), two cytokinins (kinetin, zeatin), and the fungal mycotoxin zearalenone, were tested individually in vitro using embryo-, and inflorescence-derived callus. To determine the role of oxidative stress in cell regeneration, changes in the basic antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), and peroxidases (PODs) were investigated. In general, zearalenone (ZEN) was found to be more effective than cytokinin treatments for inducing shoot production, whereas auxins suppressed the regeneration process. Regenerating callus showed higher induction of these antioxidant enzymes in comparison with non-regenerating callus. SOD, CAT and POD activities were higher in callus derived from inflorescence than in callus derived from immature embryo. Activities of SOD, CAT and POD in culture derived from immature embryos were depending on type of growth regulator in medium. The highest enzyme activities were observed in nonregenerating tissues after auxins treatment and in regenerating tissues after cytokinins treatment. The effect of ZEN was similar to that of cytokinins. One MnSOD band and two Cu/ZnSOD bands were detected in all cultures. Changes in SOD izoform patterns occurred in callus culture on media with auxins and ZEN, but not on media with cytokinins. Our results suggest that callus regeneration is associated with reactive oxygen species production induced by specific growth regulators. Reactive oxygen species under the control of cellular antioxidant machinery can mediate signalling pathways between exogenously applied growth regulators and the induction and/or creation of the direction of morphogenesis.
Słowa kluczowe
Opis fizyczny
  • Institute of Plant Physiology, Polish Academy of Sciences, Podluzna 3, 30-239 Krakow, Poland
  • Department of Botany, Stockholm University, Lilla Frescativagen 5, 10691 Stockholm, Sweden
  • Institute of Plant Physiology, Polish Academy of Sciences, Podluzna 3, 30-239 Krakow, Poland
  • Institute of General and Molecular Biology, Nicolas Copernicus University, Gagarina 9, 87-100 Torun, Poland
  • Institute of Plant Physiology, Polish Academy of Sciences, Podluzna 3, 30-239 Krakow, Poland
  • Institute of Plant Physiology, Polish Academy of Sciences, Podluzna 3, 30-239 Krakow, Poland
  • Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, Poland
  • Institute of Plant Physiology, Polish Academy of Sciences, Podluzna 3, 30-239 Krakow, Poland
  • Pedagogical University of Krakow, Podchorazych 2, 30-084 Krakow, Poland
  • Aebi H (1984) Catalase in vitro. Meth Enzymol 105:121–125
  • Asada K (1992) Ascorbate peroxidase: a hydrogen peroxide—scavenging enzyme in plants. Physiol Plant 85:235–241
  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–87
  • Benkirane H, Sabounji K, Chlyah A (2000) Somatic embryogenesis and plant regeneration from fragments of immature inflorescences and coleoptiles of durum wheat. Plant Cell Tissue Organ Cult 61:107–113
  • Biesaga-Koscielniak J, Marcinska I, Wedzony M, Koscielniak J (2003) Effect of zearalenone treatment on the production of wheat haploids via the maize pollination system. Plant Cell Rep 21:1035–1039
  • Bradford M (1976) A rapid and sensitive method for the quantitation of microprogram quantitaties of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
  • Chiappetta A, Michelotti V, Fambrini M, Bruno L, Salvini M, Petrarulo M, Azmi A, Van Onckelen H, Pugliesi C, Bitonti MB (2005) Zeatin accumulation and misexpression of a class I knoxgene are intimately linked in the epiphyllous response of the interspecific hybrid EMB-2 (Helianthus annuus H. tuberosus). Planta doi:10.1007/s00425–005–0150–7
  • Clarke SF, Burritt DJ, Jameson PE, Guy PL (2000) Influence of plant hormones on white clover mosaic potexvirus double stranded RNA. Plant Pathol 49:428–434
  • Cutler A, Saleem M, Wang H (1991) Cereal protoplast recalcitrance. In Vitro Cell Dev Biol 27:104–111
  • Daimon Y, Takabe K, Tasaka M (2003) The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on calli. Plant Cell Physiol 44:113–121
  • Danicke S, Brussow KP, Valenta H, Ueberschar KH, Tiemann U, Schollenberger M (2005) On the effects of graded levels of Fusarium toxin contaminated wheat in diets for gilts on feed intake, growth performance and metabolism of deoxynivalenol and zearalenone. Mol Nutr Food Res 49:932–943
  • Fisher C, Neuhaus G (1996) Influence of auxin on the establishment of bilateral symmetry in monocots. Plant J 9:659–669
  • Guo DP, Zhu ZJ, Hu XX, Zheng SJ (2005) Effect of cytokinins on shoot regeneration from cotyledon and leaf segment of stem mustard (Brassica juncea var. tsatsai). Plant Cell Tissue Organ Cult 83:123–127
  • Hake S, Smith HMS, Holtan H, Magnani E, Mele G, Ramirez J (2004) The role of knox genes in plant development. Annu Rev Cell Dev Biol 20:125–151
  • Halperin W (1986) Attainment and retention of morphogenetic capacity in vitro. In: Vasil IK (ed) Plant regeneration and genetic variability. Academic, London, vol 3, pp 3–47
  • Hassan HM, Fridovich I (1977) Regulation of the synthesis of superoxide dismutase in Escherichia coli. J Biol Chem 252:7667–7672
  • Hjortswang HI, Sundas Larsson A, Bharathan G, Bozhkov PV, Von Arnold S, Vahala T (2002) KNOTTED1-like homeobox genes of gymnosperm, Norway spruce, expressed during somatic embryogenesis. Plant Physiol Biochem 40:837–843
  • Iantcheva A, Slavov S, Prinsen E, Vlahova M, van Onckelen H, Atanassov A (2005) Embryo induction and regeneration from root explants of Medicago truncatula after osmotic pre-treatment. Plant Cell Tissue Organ Cult 81:37–43
  • Illés E, Szabó M, Csiszár J (2002) Stress tolerance in auxin heterotrophic and autotrophic tobacco tissue cultures Proceedings of the 7th Hungarian Congress on Plant Physiology. Acta Biol Szeged 46:83–84
  • Jiménez VM, Bangerth F (2001) Endogenous hormone levels in explants and in embryogenic and non-embryogenic cultures of carrot. Physiol Plant 111:389–395
  • Kairong C, Gengsheng X, Xinmin L, Gengmei X, Yafu W (1999) Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbanum L. Plant Sci 146:9–16
  • Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P (1999) Systemic signalling and acclimatisation in response to excess excitation energy in Arabidopsis. Science 284:654–657
  • Lattoo SK, Bamotra S, Sapru Dhar R, Khan S, Dhar AK (2005) Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker-an endangered medicinal herb. Plant Cell Rep doi:10.1007/s00299–005–0103–4
  • Li WZ, Song ZH, Guo BT, Xu LJ (2001) The effects of DNA hypomethylating drugs on androgenesis in barley (Hordeum vulgare L.). In Vitro Cell Dev Biol Plant 37:605–608
  • Limam F, Chahed K, Ouelhazi N, Ghrir R, Ouelhazi L (1998) Phytohormones regulation of isoperoxidases in Catharanthus roseus suspension culture. Phytochemistry 49:1219–1225
  • Loschiavo F, Pitto L, Giuliano G, Torti G, Nuti-Ronchi V, Marazziti D, Vergara R, Orselli S, Terzi M (1989) DNA methylation of embryogenic carrot cell cultures and its variation as caused by mutation, differentiation, hormones and hypomethylating drugs. Theor Appl Genet 77:325–331
  • Lück (1962) Methoden der enzymatischen Analyse. In: Bergmeyer HU (eds) Verlag Chemie, GmbH Weinheim, pp 895–897
  • de Marco A, Roubelakis-Angelakis KA (1996) The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol 11:137–145
  • de Marco A, Guzzardi P, Jamet E (1999) Isolation of tobacco isoperoxidases accumulated in cell-suspension culture medium and characterisation of activities related to cell wall metabolism. Plant Physiol 120:371–381
  • McCord JM, Fiodovich I (1969) Superoxide dismutase. An enzimic function for erytrocuperein (hemocuperein). J Biol Chem 244:6049–6055
  • Menke-Milczarek I, Zimny J (2001) NH⁴⁺ and NO³⁻ requirement for wheat somatic embryogenesis. Acta Physiol Plant 23:37–42
  • Menna ME, Lauren DR, Hardacre A (1997) Fusaria and Fusarium toxins in New Zealand maize plants. Mycopathologia 139:165–173
  • Michalczuk L, Ribnicky DM, Cooke TJ, Cohen JD (1992) Regulation of indole-3-acetic acid biosynthetic pathways in carrot cell cultures. Plant Physiol 100:1346–1353
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Neumann KH (2000) Some studies on somatic embryogenesis, a tool in plant biotechnology, 87th Indian Science Congress Jan. 2000 in Pune, India
  • Padmanabhan K, Cantliffe DJ, Koch KE (2001) Auxin-regulated gene expression and embryogenic competence in callus cultures of sweetpotato,Ipomoea batatas(L.) Lam. Plant Cell Rep 20:187–192
  • Pan SM, Yau YY (1992) Characterization of superoxide dismutase in Arabidopsis. Plant Cell Physiol 37:58–66
  • Papadakis AK, Roubelakis-Angelakis A (1999) The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts. Plant Physiol 121:197–205
  • Papadakis AK, Siminis Ch I, Roubelakis-Angelakis KA (2001) Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts. Plant Physiol 126:434–444
  • Pasternak TP, Prinsen E, Ayaydin F, Miskolczi P, Potters G, Asard H, Van Onckelen HA, Dudits D, Feher A (2002) The role of auxin, pH, and stress in the activation of embryogenic cell division in leaf protoplast-derived cells of Alfalfa. Plant Physiol 129:1807–1819
  • Pellegrineschi A, Brito RM, McLean S, Hoisington D (2004) Effect of 2,4-dichlorophenoxyacetic acid and NaCl on the establishment of callus and plant regeneration in durum and bread wheat. Plant Cell Tissue Organ Cult 77:245–250
  • Picciarelli P, Ceccarelli N, Paolicchi F, Calistri G (2001) Endogenous auxin and embryogenesis in Phaseolus coccineus. Aust J Plant Physiol 28:73–78
  • Qiusheng Z, Bao J, Likun L, Xianhua X (2005) Effects of antioxidants on the plant regeneration and GUS expressive frequency of peanut (Arachis hypogaea) explants by Agrobacterium tumefaciens. Plant Cell Tissue Organ Cult 81:83–90
  • Rentel MC, Knight MR (2004) Oxidative stress-induced calcium signalling in Arabidopsis. Plant Physiol 135:1471–1479
  • Roubelakis-Angelakis KA (1993) An assessment of possible factors contributing to recalcitrance of plant protoplasts. In: Roubelakis-Angelakis KA, Tranh Tan Van K (eds) Morphogenesis in plants: molecular approaches. Plenum Publishing, New York, pp 201–219
  • Scandalios JG (1993) Oxygen stress and superoxide dismutases. Plant Physiol 100:7–12
  • Schiavone FM, Cooke TJ (1987) Unusual patterns of somatic embryogenesis in the domesticated carrot: developmental effects of exogenous auxin and auxin transport inhibitors. Cell Differ 21:53–62
  • Sieberer T, Hauser MT, Seifert GJ, Luschnig C (2003) PROPORZ1, a putative Arabidopsis transcriptional adaptor protein, mediates auxin and cytokinin signals in the control of cell proliferation. Curr Biol 13:837–842
  • Siminis CI, Kanellis AK, Roubelakis-Angelakis KA (1993) Differences in protein synthesis and peroxidase isoenzymes between recalcitrant and regenerating protoplasts. Physiol Plant 87:263–270
  • Synkova H, Semoradova S, Burketova L (2004) High content of endogenous cytokinins stimulates activity of enzymes and proteins involved in stress response in Nicotiana tabacum. Plant Cell Tissue Organ Cult 79:169–179
  • Tamás C, Szücs P, Rakszegi M, Tamás L, Bedö Z (2004) Effect of combined changes in culture medium and incubation conditions on the regeneration from immature embryos of elite varieties of winter wheat. Plant Cell Tissue Organ Cult 79:39–44
  • Vergara R, Verde F, Pitto L, Loschiavo F, Terzi M (1990) Reversible variations in the methylation pattern of carrot DNA during somatic embryogenesis. Plant Cell Rep 8:697–700
  • Wang H, Meng FJ (1990) Formation of endogenous zearalenone and it inhibition by malathion in winter wheat during vernalization. Acta Phytophysiol Sin 16:197–200
  • Willekens H, Inze D, Van Montagu M, Van Camp W (1995) Catalases in plants. Mol Breed 1:207–228
  • Willekens H, Chamnongpol SCh, Davey M, Schraudner M, Langebartels Ch Van Montagu M, Inze D, Van Camp W (1997) Catalase is a sink for H₂O₂ and is indispensable for stress defense in C3 plants. EMBO J 16:4806–4816
  • Wu IF, Chen JT, Chang W Ch (2004) Effects of auxins and cytokinins on embryo formation from root-derived callus of Oncidium ‘Gower Ramsey’. Plant Cell Tissue Organ Cult 77:107–109
  • Zhang S, Wong L, Meng L, Lemaux PG (2002) Similarity of expression patterns of knotted1 and ZmLEC1 during somatic and zygotic embryogenesis in maize (Zea mays L.). Planta 215:191–194
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.