Functional and transcriptomic characterization of a dye-decolorizing fungus from Taxus rhizosphere

• Autorzy: Hao D.C. , Song S.M. , Cheng Y. , Qin Z.Q. , Ge G.B. , An B.L. , Xiao P.G.
• Języki publikacji: EN

Abstrakty

EN

We isolated three laccase-producing fungus strains from Taxus rhizosphere. Myrotheium verrucaria strain DJTU-sh7 had the highest laccase activity of 216.2 U/ml, which was increased to above 300 U/ml after optimization. DJTU-sh7 had the best decolorizing effect for three classes of reactive dyes. The DJTU-sh7-containing fungal consortium displayed the robust decolorizing ability. Both color removal efficiency and chemical oxygen demand were increased in the consortium mediated biotransformation. Transcriptome changes of M. verrucaria elicited by azo dye and phenolic were quantified by the high throughput transcriptome sequencing, and the activities of the selected oxidases and reductases were determined. The possible involvement of oxidases and reductases, especially laccase, aryl alcohol oxidase, and ferric reductase in the biotransformation of dye and phenolic compounds was revealed at both transcriptomic and phenotypic levels. Revealing the transcriptomic mechanisms of fungi in dealing with organic pollutants facilitates the fine-tuned manipulation of strains in developing novel bioremediation and biodegradation strategies.

Słowa kluczowe

EN

fungi; functional characteristics; transcriptomics; dye decolorization; laccase; Taxus; rhizosphere; Myrothecium verrucaria; bioremediation

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2018
• Tom: 67
• Numer: 4
• Opis fizyczny: p.417-429,fig.,ref.

Twórcy

autor

Hao D.C.

• Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian, China

autor

Song S.M.

• Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian, China

autor

Cheng Y.

• Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian, China

autor

Qin Z.Q.

• Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian, China

autor

Ge G.B.

• Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, China
• Shanghai University of Traditional Chinese Medicine, Shanghai, China

autor

An B.L.

• Biotechnology Institute, School of Environment and Chemical Engineering, Dalian Jiaotong University, Dalian, China

autor

Xiao P.G.

• Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China

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Identyfikatory

DOI

10.21307/pjm-2018-050