PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2002 | 07 | 2A |
Tytuł artykułu

Studies on changes in specific rye genome regions due to seed aging and regeneration

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to identify the genetic changes in rye seeds induced by natural aging during long-term storage and successive regeneration cycles under gene bank conditions. Genomic DNA from four rye samples (cv. Dańkowskie Złote), varying in their initial viability and having gone through one or three reproduction cycles, were analysed using specific PCR targeting of a secalin locus, and various repetitive fragments defined by the R173 sequence. A statistical analysis of the band frequencies for both secalin and R173.3 primer pairs revealed no changes in their frequencies. Similar data on R173.1 demonstrated significant changes between samples of different initial viability showing a lack of a band of the expected length (987 bp) in progeny originating from low viability seeds lots. These changes were inherited even after three regeneration cycles. Our results may indicate that long-term storage that leads to loss of viability also generates heritable changes in the preserved germplasm. However, it remains to be discovered where these changes occur and whether they are connected with coding or with non-coding DNA regions.
Wydawca
-
Rocznik
Tom
07
Numer
2A
Opis fizyczny
p.569-576,fig.,ref.
Twórcy
  • The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland
  • The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland
autor
  • The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02-973 Warsaw, Poland
Bibliografia
  • 1. Schoen, D. J., David, J. L, and Bataillon, T. M. Deleterious mutation accumulation and the regeneration of genetic resources. Proc. Natl. Acad. Sci. USA 95 (1998) 394-399.
  • 2. Roos, E. E. Report of the storage committee working group on: "Effects of storage on genetic integrity" 1980-1983. Seed Sci. Technol. 12 (1984) 255-269.
  • 3. Roos, E. E. Long-term seed storage. Plant Breed. Rev. 7 (1989) 129-158.
  • 4. Roos, E. E. Genetic changes in a collection over time. Hort. Sci. 23 (1988) 86-90.
  • 5. Shewry, P. R. and Field, J. M. The purification and characterisation of two groups of storage proteins (Secalins) from rye (Secale cereale L.). J. Exp. Bot. 33 (1982) 261-268.
  • 6. Guidet, F., Rogowsky, P., Taylor, Ch., Song, W. and Langride, P. Cloning and characterisation of a new rye-specific repeated sequence. Genome 34 (1991) 81-87.
  • 7. Rogowsky, P. M., Manning, S., Liu, J-Y. and Langride, P. The R173 family of rye-specific repetitive DNA sequences: a structural analysis. Genome 34 (1991) 88-95.
  • 8. Rogowsky, P. M., Shepherd, K. W. and Langride, P. Polymerase Chain Reaction based mapping of rye involving repeated DNA sequences. Genome 35 (1992) 621-626.
  • 9. Roos, E. E. Physiological, biochemical, and genetical changes in seed quality during storage. Hort. Sci. 15 (1980) 781-784.
  • 10. Roos, E. E. Induced genetic changes in seed germplasm during storage. In: The Physiology and Biochemistry of Seed Development, Dormancy and Germination, Elsevier Biomedical Press, Amsterdam (1982) 409-434.
  • 11. Puchalski, J. Izoenzymy jako markery zmian genetycznych w siewkach żyta wywołanych przechowywaniem i regeneracją. Prace Ogrodu Botanicznego PAN. Seria: Monografie i rozprawy 4 (1993) 86.
  • 12. Bednarek, P. T., Chwedorzewska, K. and Puchalski, J. Preliminary molecular studies on genetic changes in rye seeds due to long - term storage and regeneration. In: Challenges in Rye Germplasm Conservation (Gass, T., Podyma, W., Puchalski, J. and Eberhart, S. A Eds) International Plant Genetic Resources Institute, (1998) 54-61.
  • 13. Chwedorzewska, K. J., Bednarek, P. T. and Puchalski, J. Wykorzystanie markerów RAPD do analizy zmian genetycznych związanych z długotrwałym przechowywaniem i regeneracją ziarniaków żyta (Secale cereale L.). Zeszyty Problem. Post. Nauk Roln. 463 (1998) 519-528.
  • 14. Chwedorzewska, K. J., Bednarek, P. T. and Puchalski, J. AFLP-profiling of long-term stored and regenerated rye genebank samples. Cell. Mol. Biol. Let. (2002) this issue.
  • 15. Kreis, M. Forde, B. G., Rahman, S., Miflin, B. J. and Shewry, P. R. Molecular Evolution of the seed storage proteins of barley, ray and wheat. J. Mol. Biol. 183 (1985) 499-502.
  • 16. Stoyanova, S. D. Genetic shift and variations of gliadins induced by seed aging. Seed Sci. Technol. 19 (1991) 363-371.
  • 17. Stoyanova, S. D. Effects of seed aging and regeneration on the genetic composition of wheat. Seed Sci. Technol. 20 (1992) 489-496.
  • 18. Shatters, R. G. Jr., Schweder, M. E., West, S. H., Abdelghany, A. and Smith, R. L. Environmentally induced polymorphisms detected by RAPD analysis of soybean seed DNA. Seed Sci. Res. 5 (1995) 106-116.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d0f7b51d-c444-4cad-882f-a28e7d760b82
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.