PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 09 |
Tytuł artykułu

In vitro culture and fructan production by Vernonia herbacea (Asteraceae)

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Vernonia herbacea is a native species from the Brazilian Cerrado that accumulates about 80 % of inulin-type fructans in the underground reserve organs, the rhizophores. This work aimed at establishing a protocol for in vitro culture of V. herbacea, using seeds (achenes) and leaf discs as explants. Following germination and seedling growth, stem nodes from 6-month-old in vitro germinated plants were isolated and incubated on culture medium free of growth regulators for plant propagation and rhizophore formation. Fructan content and composition were evaluated in leaves, stems, roots and rhizophores from plants grown in vitro and compared with those of greenhouse-grown plants, in order to evaluate inulin production in vitro. Fructan contents of aerial organs and roots from in vitro plants were higher, compared with greenhouse plants, while in rhizophores, the opposite was observed. High performance anion exchange chromatography/pulsed amperometric detection profiles revealed the presence of the inulin homologous series in the aerial organs exclusively for in vitro plants, while in roots and rhizophores, this series was detected in plants grown in both conditions. These results indicate a modification in the source/sink ratio, leading to changes in the distribution of carbohydrates in in vitro plants. The leaf disc cultures on medium supplemented with indole-3-butyric acid induced the formation of roots (0.24, 0.49 µM) and friable callus (2.46 µM), while 6-benzylaminopurine (from 1.1 through 4.43 µM) induced compact callus. However, no shoot formation was observed. The use of seeds allowed the establishment of a protocol for in vitro culture and provides a model system for a better understanding of fructan metabolism in V. herbacea.
Wydawca
-
Rocznik
Tom
36
Numer
09
Opis fizyczny
p.2299-2307,fig.,ref.
Twórcy
autor
  • Nucleo de Pesquisa em Fisiologia e Bioquimica, Instituto de Botanica, PO Box 68041, Sao Paulo, SP CEP 04045-972, Brazil
autor
  • Nucleo de Pesquisa em Fisiologia e Bioquimica, Instituto de Botanica, PO Box 68041, Sao Paulo, SP CEP 04045-972, Brazil
autor
  • Nucleo de Pesquisa em Fisiologia e Bioquimica, Instituto de Botanica, PO Box 68041, Sao Paulo, SP CEP 04045-972, Brazil
  • Nucleo de Pesquisa em Fisiologia e Bioquimica, Instituto de Botanica, PO Box 68041, Sao Paulo, SP CEP 04045-972, Brazil
Bibliografia
  • Asega AF, Nascimento JRO, Schroeven L, Van den Ende W, Carvalho MAM (2008) Cloning, characterization and funcional analysis of 1-FEH cDNA from Vernonia herbacea (Vell.) Rusby. Plant Cell Physiol 49:1185–1195
  • Asega AF, Nascimento JR, Carvalho MA (2011) Increased expression of fructan 1-exohydrolase in rhizophores of Vernonia herbacea during sprouting and exposure to low temperature. J Plant Physiol 168:558–565
  • Bausewein A, Greiner S, Harms K, Rausch T (2012) Hormonal regulation of fructan active enzymes in chicory (Cichorium intybus) (Abstract). In: Presented at the 7th international fructan symposium, July 2–6 2012, Saint-Jean-le-Thomas, France Carvalho MAM, Dietrich SMC (1993) Variation in fructan content from underground organs of Vernonia herbacea (Vell.) Rusby in different phenological phases. New Phytol 123:735–740
  • Carvalho MAM, Pinto MM, Figueiredo-Ribeiro RCL (1998) Inulin production by Vernonia herbacea as influenced by mineral fertilization and time of harvest. Rev Bras Bot 21:281–285
  • Corrêa MC, Oliveira GN, Astarita LV, Santarém ER (2009) Plant regeneration through somatic embryogenesis of yacón [Smallanthus sonchifolius (Poepp. and Endl.) H. Robinson]. Braz Arch Biol Techn 52:549–554
  • Cuzzuol GRF, Carvalho MAM, Barbedo CJ, Zaidan LBP (2003) Crescimento e produção de frutanos em plantas de Vernonia herbacea (Vell.) Rusby submetidas à adubação nitrogenada. Rev Bras Bot 26:81–91
  • Cuzzuol GRF, Carvalho MAM, Zaidan LBP, Furlani PR (2005) Soluções nutritivas para cultivo e produção de frutanos em plantas de Vernonia herbacea. Pesq Agropec Bras 40:911–917
  • Dias-Tagliacozzo GM, Itaya NM, Carvalho MAM, Figueiredo-Ribeiro RCL, Dietrich SMC (2004) Fructans and water suppression on intact and fragmented rhizophores of Vernonia herbacea. Braz Arch Biol Technol 47:363–373
  • Ehneß R, Roitsch T (1997) Co-ordinated induction of mRNAs for extracellular invertase and a glucose transporter in Chenopodium rubrum by cytokinins. Plant J 11:539–548
  • Garcia PMA, Asega AF, Silva EA, Carvalho MAM (2011) Effect of drought and re-watering on fructan metabolism in Vernonia herbacea (Vell.) Rusby. Plant Physiol Biochem 49:664–670
  • George EF, Hall MA, De Klerk G-J (2008) Plant propagation by tissue culture, 3rd edn. Springer, Dordrecht
  • Grigoriadou K, Krigas N, Maloupa E (2011) GIS-facilitated in vitro propagation and ex situ conservation of Achillea oculta. Plant Cell Tiss Organ Cult 107:531–540
  • Haaß D, Abou-Mandour AA, Blaschek W, Franz G, Czygan FC (1991) The influence of phytohormones on growth, organ differentiation and fructan production in callus of Symphytum officinale L. Plant Cell Rep 10:421–424
  • Hale AD, Pollock CJ, Dalton SJ (1987) Polysaccharide production in liquid cell suspension cultures of Phleum pratense L. Plant Cell Rep 6:435–438
  • Hendry GAF, Wallace RK (1993) The origin, distribution and evolutionary significance of fructans. In: Suzuki M, Chatterton JN (eds) Science and technology of fructans. CRC Press, Boca Raton, pp 119–139
  • Hincha DK, Livingston DP, Premakumar R, Zuther E, Obel N, Cacela C, Heyer AG (2007) Fructans from oat and rye: composition and effects on membrane stability during drying. Biochim Biophys Acta 1768:1611–1619
  • Irvani N, Solouki M, Omidi M, Zare AR, Shahnazi S (2010) Callus induction and plant regeneration in Dorem ammoniacum D., an endangered medicinal plant. Plant Cell Tiss Organ Cult 100: 293–299
  • Itaya NM, Vaz APA, Kerbauy GB, Figueiredo-Ribeiro RCL (2005) Produção de frutanos em calos e plântulas clonadas in vitro de Viguiera discolor Baker (Asteraceae). Acta Bot Bras 19:579–586
  • Jermyn MA (1956) A new method for the determination of ketohexoses in presence of aldohexoses. Nature 177:38–39
  • Khalafalla MM, Elgaali EI, Ahmed MM (2007) In vitro multiple shoot regeneration from nodal explants of Vernonia amygdalina—an important medicinal plant. Afr Crop Sci Conf Proc 8:747–752
  • Koda Y, Okazawa Y (1983) Influences of environmental, hormonal and nutritional factors on potato tuberization in vitro. Jpn J Crop Sci 53:582–591
  • Kusch U, Greine S, Meyer AD, Corbière-Divialle H, Harms K, Rausch T (2009) Dissecting the regulation of fructan metabolism in chicory (Cichorium intybus) hairy roots. New Phytol 184:127–140
  • Le Guen-Le Saos F, Hourmant A, Esnault F, Chauvin JE (2002) In vitro bulb development in shallot (Allium cepa L. Aggregatum Group): effects of anti-gibberellins, sucrose and light. Ann Bot 89: 419–425
  • Livingston DP III, Hincha DK, Heyer AG (2009) Fructan and its relationship to abiotic stress tolerance in plants. Cell Mol Life Sci 66:2007–2023
  • Mercier H, Vieira CCJ, Figueiredo-Ribeiro RCL (1992) Tissue culture and plant propagation of Gomphrena officinalis—a Brazilian medicinal plant. Plant Cell Tiss Org Cult 28:249–254
  • Moreira MF, Appezzato-Da-Gloria B, Zaidan BP (2000) Anatomical aspects of IBA-treated microcuttings of Gomphrena macrocephala St.-Hil. Braz Arch Boil Technol 43:221–227
  • Murashige TQ, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plantarum 15: 473–479
  • Noshad D, Miresmaili S, Riseman A, Ekramoddoullah A (2009) In vitro propagation of seven Daphne L. species. Plant Cell Tiss Organ Cult 96:201–209
  • Oliveira VF, Zaidan LBP, Braga MR, Aidar MPM, Carvalho MAM (2010) Elevated CO₂ atmosphere promotes plant growth and inulin production in the cerrado species Vernonia herbacea. Funct Plant Biol 37:223–231
  • Perez-Tornero O, Tallon CI, Porras I (2010) An efficient protocol for micropropagation of lemon (Citrus limon) from mature nodal segments. Plant Cell Tiss Organ Cult 100:263–271
  • Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Holanda
  • Pugliesi C, Megale P, Cecconi F, Baroncelli S (1993) Organogenesis and embryogenesis in Helianthus tuberosus and in the interspecific hybrid Helianthus annuus x Helianthus tuberosus. Plant Cell Tiss Organ Cult 33:187–193
  • Rehman RU, Israr M, Srivastava PS (2003) In vitro regeneration of witloof chicory (Cichorium intybus L.) from leaf explants and accumulation of esculin. In Vitro Cell Dev-Pl 39:142–146
  • Ritsema T, Smeekens S (2003) Fructans: beneficial for plants and humans. Curr Opin Plant Biol 6:223–230
  • Roberfroid MB (2005) Introducing inulin-type fructans. Br J Nutr 93:13–25
  • Sassaki RM, Rondon JN, Zaidan LBP, Felippe GM (1999) Germination of seeds from herbaceous plants artificially stored in Cerrado soil. R Bras Biol 59:271–279
  • Súarez-González EM, López MG, Délano-Frier JP, Gómez-Leyva JF (2013) Expression of the 1-SST and 1-FFT genes and consequent fructan accumulation in Avave tequilana and A. inaequidens is differentially induced by diverse (a) biotic-stress related elicitors. J Plant Physiol. doi:10.1016/j.jplph.2013.08.002
  • Taha HS, El-sawy AM, Bekheet SA (2007) In vitro studies on Jerusalem artichoke (Helianthus tuberosus) and enhancement of inulin production. J Appl Sci Res 3:853–858
  • Van den Ende W (2013) Multifunctional fructans and raffinose family oligosaccharides. Front Plant Sci 4:247. doi:10.3389/fpls.2013.00247
  • Van den Ende W, Michiels A, De Roover J, Van Laere A (2002) Fructan biosynthetic and breakdown enzymes in dicots evolved from different invertases. Expression of fructan genes through chicory development. Sci World J 2:1281–1295
  • Vieira CJ, Braga MR, Figueiredo-Ribeiro RCL (1995) Fructans in callus of Gomphrena macrocephala St.-Hill. Plant Cell Tiss Organ Cult 42:233–238
  • Wimken A, Sprenger N, Boller T (1995) Fructans-an extension of sucrose by sucrose. In: Pontis HG, Salerno GL, Echeverria ES (eds) International symposium on sucrose metabolism. American Society of Plant Physiologists, Rockville, pp 179–189
  • Zhou H, Li M, Zhao X, Fan X, Guo A (2010) Plant regeneration from in vitro leaves of the peach rootstock ‘Nemaguard’ (Prunus persica × P. davidiana). Plant Cell Tiss Organ Cult 101:79–87
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-d0e22ea4-f02f-4053-b47e-e534763170a9
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.