HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds

• Autorzy: Lin H , Liu G. , Fu J. , Wang A.
• Języki publikacji: EN

Abstrakty

EN

Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods.Three Huai and three Landrace pigs each were individually housed at the Fujian Shanghang Huai pig breeding farm under similar feeding conditions. Pigs were humanely slaughtered at saleable weight and samples of kidney, liver and skeletal muscle were collected for expression analyses.HMGR mRNA expressions in liver, muscle and kidney of Huai pigs both occurred higher than expression found in the Landrace. Positive staining of HMGR protein was revealed in the three tissues considered. The HMGR protein expression level was not significant statistically in liver,muscle and kidney across two breeds, but the amount of Huai pig HMGR protein expressed in three tissues was higher than that of the Landrace. The level of HMGR protein expression in liver was higher than in the muscle and renal tissues (P<0.05). The results demonstrate that HMGR expression in pigs may depend on tissue and species.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2013
• Tom: 31
• Numer: 1
• Opis fizyczny: p.35-43,fig.,ref.

Twórcy

autor

Lin H

• College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
• Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
• Shandong Key Lab of Animal Disease Control and Breeding, Jinan, China

autor

Liu G.

• College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
• Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

autor

Fu J.

• College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

autor

Wang A.

• College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China

Bibliografia

• BACH T.J. 1995 – Some new aspects of isoprenoid biosynthesis in plants – A review. Lipids. 30,191-202.
• DENBOW C.J., SAARA L., CAROLE L.C., 1996 – The N-terminal domain of tomato 3-hydroxy-3-methylglutaryl-CoA reductase. Journal of Biological Chemistry 271(16), 9710-9715.
• DORADO M., MARTIN GOMEZ E.M., JIMENEZ-COLMENERO F., MASOUDB T.A., 1999 – Cholesterol and fat contents of Spanish commercial pork cuts. Meat Science 51, 321-323.
• DUCKWORTH P.F., VLAHCEVIC Z.R., STUDER E.J., 1991 – Effect of hydrophobic bile acid on 3-hydroxy-3-methyl- glutaryl-CoA reductase activity and mRNA levels in the rat. Journal of Biological Chemistry 266, 9413-9418.
• GASTALDELLI A, KOZAKOVA M, HOJLUND K, FLYVBJERG A, FAVUZZI A, MITRAKOU A, BALKAU B; RISC Investigators 2009 –. Fatty liver is associated with insulin resistance, risk of coronary heart disease, and early atherosclerosis in a large European population. Hepatology 49(5),1537-1544.
• GOLDWASSER J., COHEN P.Y., YANG E., BALAGUER P., YARMUSH M.L. & NAHMISA YAAKOV., 2010 – Transcriptional regulation of human and rat hepatic lipid metabolism by the grapefruit flavonoid natingenin: role of PPARα, PPARγand LXRα. PLoS ONE 5(8), e12399.
• HARRIS K.B., POND W.G., MERSMANN H.J., SMITH E.O., CROSSA H.R.., SAVELLA J.W.,2003. – Evaluation of fat sources on cholesterol and lipoproteins using pigs selected for high or low serum cholesterol. Meat Science 66, 55-61.
• HUMPHRIES S.E., TATA F., HENRY I., 1985 – The isolation, characterization, and chromosomal assignment of the gene for human hydroxy3-methylglutaryl coenzyme A reductase (HMG-CoA reductase). Human Genetics 71, 254-258.
• Istvan E.S., Deisenhofer J., 2000 – The structure of the catalytic portion of human HMG-CoA reductase. Biochimica et Biophysica Acta 1529(1-3), 9-18.
• JIANG J.H., KAI G..Y., CAO X.Y., CHEN F.M., HE D.N., LIU Q., 2006 –Molecular cloning of a HMG-CoA reductase gene from Eucommia ulmoides Oliver. Bioscience Reports 26, 171-181.
• LIU G.F., WANG A.G., Fu J.L., 2008 – Study on the Characteristics of Tissue Expression of 3-hydroxy-3-methylglutaryl Coenzyme A in Different Pig Breeds. Acta Ecologiae Animalis Domastici 29(3): 16-19.
• LUSKEY K.L., STEVENS B., 1985 – Human 3-hydroxy-3-methylglutaryl coenzyme A reductase.Conserved domains responsible for catalytic activity and sterol-regulated degradation. Journal of Biological Chemistry 260, 10271-10277.
• MYANT N.B. 1990 – Cholesterol metabolism, LDL, and the LDL receptor. Academic Press, San Diego.
• RAI S.K., SHARMA M., TIWARI M., 2009 – Inhibitory effect of novel diallyldisulfide analogs on HMG-CoA reductase expression in hypercholesterolemic rats, CREB as a potential upstream target.Life Science 85(5-6), 211-219.
• SATO K., OHUCHI A., SOOK S.H., TOOMIZU M., AKIBA Y., 2003 – Changes in mRNA expression of 3-hydroxy- 3-methylglutaryl coenzyme A reductase and cholesterol 7 alpha-hydroxylase in chickens. Biochimica et Biophysica Acta 1630, 96-102.
• Simonet W.S., Ness G..C., 1988 – Transcriptional and post-transcriptional regulation of rat hepatic 3-hydroxy-3-methylglutaryl-CoA reductase by thyroid hormones. Journal of Biological Chemistry 263, 12448-12453.
• SUNDARESAN S., YANG-FENG T.L., FRANCKE U., 1989 – Genes for HMG-CoA reductase and serotonin 1a receptor are on mouse chromosome 13. Somatic Cells and Molecular Genetics 15,465-469.