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2013 | 31 | 1 |
Tytuł artykułu

HMGR messenger RNA and protein expression in liver, kidney and muscle in pigs of two breeds

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Treść / Zawartość
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Języki publikacji
EN
Abstrakty
EN
Distribution and expression of the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) in different breeds and tissues of pigs were studied by means of immunohistochemical and RT-PCR methods.Three Huai and three Landrace pigs each were individually housed at the Fujian Shanghang Huai pig breeding farm under similar feeding conditions. Pigs were humanely slaughtered at saleable weight and samples of kidney, liver and skeletal muscle were collected for expression analyses.HMGR mRNA expressions in liver, muscle and kidney of Huai pigs both occurred higher than expression found in the Landrace. Positive staining of HMGR protein was revealed in the three tissues considered. The HMGR protein expression level was not significant statistically in liver,muscle and kidney across two breeds, but the amount of Huai pig HMGR protein expressed in three tissues was higher than that of the Landrace. The level of HMGR protein expression in liver was higher than in the muscle and renal tissues (P<0.05). The results demonstrate that HMGR expression in pigs may depend on tissue and species.
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-
Rocznik
Tom
31
Numer
1
Opis fizyczny
p.35-43,fig.,ref.
Twórcy
autor
  • College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
  • Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
  • Shandong Key Lab of Animal Disease Control and Breeding, Jinan, China
autor
  • College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
  • Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Jinan, 250100, China
autor
  • College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
autor
  • College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China
Bibliografia
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Bibliografia
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