An efficient, widely applicable cryopreservation of Lilium shoot tips by droplet vitrification

• Autorzy: Yin Z.-F. , Bi W.-L. , Chen L. , Zhao B. , Volk G.M. , Wang Q.-C.
• Języki publikacji: EN

Abstrakty

EN

We report a straightforward and widely applicable cryopreservation method for Lilium shoot tips. This method uses adventitious shoots that were induced from leaf segments cultured for 4 weeks on a shoot regeneration medium containing 1 mg/l α-naphthaleneacetic acid and 0.5 mg/l thidiazuron. Shoot tips (1.5–2 mm in length) including 2–3 leaf primordia were precultured on Murashige and Skoog (MS; 1962) medium with 0.5 M sucrose for 1 day and then treated with a loading solution containing 0.4 M sucrose and 2 M glycerol for 20 min, followed by a Plant Vitrification Solution 2 (PVS2) treatment for 4 h at 0°C. Dehydrated shoot tips were transferred onto 2.5 μl PVS2 droplets on aluminum foil strips, prior to a direct immersion into liquid nitrogen for 1 h. Frozen shoot tips were re-warmed in MS medium containing 1.2 M sucrose for 20 min at room temperature, followed by post-thaw culture for shoot regrowth. Shoot regrowth levels ranged from 42.5 % for L. longiflorum × Oriental ‘Triumphator’ to 87.5 % for L. Oriental hybrid ‘Siberia’, with a mean shoot regrowth level of 67.1 % across the six diverse Lilium genotypes tested. Histological observations found that the survival patterns were similar in cryopreserved shoot tips of ‘Triumphator’ and ‘Siberia’. Assessments using inter-simple sequence repeat markers found no differences in regenerants recovered from the control stock cultures and from cryopreserved shoot tips in ‘Triumphator’ and ‘Siberia’. This Lilium droplet-vitrification cryopreservation method is efficient, simple and widely applicable for the long-term conservation of lily genetic resources.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 07
• Opis fizyczny: p.1683-1692,fig.,ref.

Twórcy

autor

Yin Z.-F.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of al Crops of Northwest China, College of Horticulture, Northwest A and F University, 712100 Yangling, Shaanxi, People's Republic of China

autor

Bi W.-L.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of al Crops of Northwest China, College of Horticulture, Northwest A and F University, 712100 Yangling, Shaanxi, People's Republic of China

autor

Chen L.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of al Crops of Northwest China, College of Horticulture, Northwest A and F University, 712100 Yangling, Shaanxi, People's Republic of China

autor

Zhao B.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of al Crops of Northwest China, College of Forestry, Northwest A and F University, 712100 Yangling, Shaanxi, People's Republic of China

autor

Volk G.M.

• USDA-ARS National Center for Genetic Resources Preservation, 111 S. Mason St., Fort Collins, 80521 CO, USA

autor

Wang Q.-C.

• State Key Laboratory of Crop Stress Biology for Arid Areas, Key Laboratory of Genetic Improvement of al Crops of Northwest China, College of Horticulture, Northwest A and F University, 712100 Yangling, Shaanxi, People's Republic of China

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Identyfikatory

DOI

10.1007/s11738-014-1543-7