Time-lapse video and electrophysiological methods in studies of circumnutation in plants

• Autorzy: Stolarz M. , Dziubinska H. , Trebacz K.
• Języki publikacji: EN

Abstrakty

EN

In specialised sensitive plants such as Mimosa, Dionaea, and Aldrovanda, rapid organ movement is observed and the excitationturgor loss mechanism is the basis for rapid leaf or trap closure. In non-specialised ubiquitous plants, slow movement named circumnutation is common and it is driven by turgor. We examine whether transmembrane potential changes such as oscillations and long distance signal action and variation potentials are involved in this movement. Video camera recordings combined with extracellular measurement of electrical potential changes are applied. Additionally, intracellular microelectrodes and patchclamp measurements are engaged. Novel software Circumnutation Tracker has been developed to track organ movement and standardisation of circumnutation parameters. Helianthus annuus and Arabidopsis thaliana are studied and a model of the circumnutation mechanism is proposed.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: p.339

Twórcy

autor

Stolarz M.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

autor

Dziubinska H.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland

autor

Trebacz K.

• Department of Biophysics, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University, Lublin, Poland