Exogenous application of gibberellic acid mitigates drought-induced damage in spring wheat

• Autorzy: Moumita , Al Mahmud J. , Biswas P.K. , Nahar K. , Fujita M. , Hasanuzzaman M.

Warianty tytułu

PL

Egzogenna aplikacja kwasu giberelinowego łagodzi uszkodzenia pszenicy jarej wywołane suszą

• Języki publikacji: EN

Abstrakty

EN

Drought stress is a major problem in wheat production but it could be managed by using various exogenous protectants such as gibberellic acid (GA). Although GA is a plant growth hormone, it shows a potential to protect the plant in stress conditions. To investigate the possible role of GA in mitigating drought stress, we treated wheat (Triticum aestivum ‘BARI Gom-21’) seedlings with a GA spray under semihydroponic conditions. In the experiment, the combined effect of GA and drought stress (induced by 12% polyethylene glycol) was studied after 48 h and 72 h. In the absence of exogenous GA, drought-stressed wheat seedlings showed various physiological and biochemical changes in a time-dependent manner. Malondialdehyde (MDA), hydrogen peroxide (H2O2) and free proline (Pro) concentrations were increased, whereas catalase (CAT) and ascorbate peroxidase (APX) activities were reduced under drought stress. Gibberellic acid played a role in restoring the ascorbate (AsA) level, decreased the reduced/oxidized glutathione (GSH/GSSG) ratio and reduced monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) activities. Gibberellic acid significantly affected the glyoxalase system. Under drought stress, the methylglyoxal (MG) concentration was increased but GA application stimulated glyoxalase I (Gly I) and glyoxalase II (Gly II) activities to protect the wheat seedlings against stress. The study concluded that the severity of drought stress in wheat depends on the growth stage and it increases with an increase in the duration of stress, whereas exogenous GA helped the seedlings to survive by upregulating antioxidant defense mechanisms and the glyoxalase system.

PL

Stres spowodowany suszą jest głównym problemem w produkcji pszenicy, ale można mu zapo- biegać przy pomocy różnych egzogennych środków ochronnych, takich jak kwas giberelinowy (GA). Chociaż GA jest fitohormonem, wykazuje potencjalne działanie ochronne w stosunku do roślin rosnących w warunkach stresowych. W celu zbadania ewentualnego oddziaływania GA w łagodzeniu stresu suszy, sadzonki pszenicy (Triticum aestivum‘BARI Gom-21’) traktowaliśmy GA w formie oprysku w warunkach pół-hydroponicznych. W przeprowadzonym doświadczeniu badano łączny wpływ GA i stresu suszy (indukowanego przez 12% glikol polietylenowy) po 48 godzinach i 72 godzinach. W siewkach pszenicy poddanych działaniu suszy, pod nieobecność egzogennego GA, stwierdzono różnorodne zmiany fizjologiczne i biochemiczne, uzależnione od czasu ekspozycji. Pod wpływem suszy stężenia dialdehydu malonowego (MDA), nadtlenku wodoru (H2O2) oraz wolnej proliny (Pro) zwiększały się, podczas gdy aktywność katalazy (CAT) i peroksydazy askorbinianowej (APX) uległa zmniejszeniu. Kwas giberelinowy odgrywał rolę w przywracaniu prawidłowego poziomu askorbinianu (AsA), zmniejszał stosunek glutationu zredukowanego/utlenionego (GSH/GSSG) oraz obniżał aktywności reduktazy monodehydro- askorbinianowej (MDHAR) i reduktazy dehydroaskorbinianowej (DHAR). Kwas giberelinowy istotnie wpłynął na układ glioksalazy. Pod wpływem stresu suszy stężenie metyloglioksalu (MG) wzrosło, ale aplikacja GA stymulowała aktywność glioksalazy I (Gly I) i glioksalazy II (Gly II) chroniąc siewki pszenicy przed stresem. W badaniach wykazano, że natężenie stresu suszy u pszenicy zależy od etapu wzrostu i nasila się wraz z wydłużeniem czasu trwania stresu, podczas gdy egzogenny GA zwiększa możliwość przetrwania siewek poprzez wzmocnienie mechanizmów obrony antyoksydacyjnej i regulację systemu glioksalazy.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2019
• Tom: 72
• Numer: 2
• Opis fizyczny: Article: 1776 [18 p.], fig.,ref.

Twórcy

autor

Moumita

• Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

autor

Al Mahmud J.

• Department of Agroforestry and Environmental Science, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

autor

Biswas P.K.

• Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

autor

Nahar K.

• Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

autor

Fujita M.

• Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa 761-0795, Japan

autor

Hasanuzzaman M.

• Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka 1207, Bangladesh

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Identyfikatory

DOI

10.5586/aa.1776