Expression of calretinin and calbindin in the cingulate cortex of the guinea pig

• Autorzy: Hermanowicz-Sobieraj B. , Bogus-Nowakowska K. , Rowniak M. , Robak A.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: The cingulate cortex (CC), a part of the limbic cortex, is one of the major components of the Papez circuit. Mammalian cerebral cortex contains excitatory pyramidal neurons (70–80%), which use glutamate as neurotransmiter, and interneurons (20–30%), mostly inhibitory, using GABA as principal neurotransmiter. The maintenance a balance between these neurotransmitters is essential for proper functioning of neurons. GABAergic neurons deficit is often related to neurodegenerative disorders. Markers for GABAergic neurons are calcium-binding proteins: calretinin (CR) and calbindin (CB), which may act as calcium sensors as well as both fast and slow calcium buffers. AIM(S): The aim of the study was to describe the distribution of CR and CB and compare expression of both CaBPs at transcriptional and final product levels in the cingulate cortex of the adult guinea pig. METHOD(S): Genes expression of CR and CB was measured on mRNA by quantitative real-time PCR (qPCR) analysis. Total RNA was isolated using Total RNA Mini and then was reverse transcribed to cDNA using Maxima cDNA Synthesis Kit. qPCR was conducted using SYBR® Green JumpStartTMTaqReadyMixTM. To visualize CR and CB immunoreactivity frozen sections were undergone for routine single immunofluorescence labelling, using solution of antibodies raised against CR and CB. RESULTS: The immunohistochemical study indicates the presence of CR and CB in the whole CC. The number of CB-positive perikarya was lower than the CR ones. CR-positive perikarya in comparison to CB-positive, were more numerous in the superficial than in the deep layers of the CC. The qPCR analysis showed that the mRNA expession for CR was higher than for CB. CONCLUSIONS: The CB and CR mRNA expression level revealed by qPCR correlate with their protein abundance level revealed by immunohistochemistry. Calretinin expression was higher than calbindin at both levels. FINANCIAL SUPPORT: Co‑financing the scientific studies for young scientists or PhD students at the Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn (ID:12.620.026-300).

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.110-111

Twórcy

autor

Hermanowicz-Sobieraj B.

• Department of Comparative Anatomy, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Bogus-Nowakowska K.

• Department of Comparative Anatomy, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Rowniak M.

• Department of Comparative Anatomy, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland

autor

Robak A.

• Department of Comparative Anatomy, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland