Attempted rescue of circling mice by hair cell-specific expression (Myo7a promoter) of tmie transgene

• Autorzy: Park S. , Jang S. , Lee J.-H. , Kim S.;H. , Ryoo Z.Y.
• Języki publikacji: EN

Abstrakty

EN

The spontaneous mutant circling mouse (cir/cir) is deaf and displays abnormal behavior, particularly circling and head tossing. To rescue the circling mouse phenotype, we produced transgenic mice with hair cell-specific expression of the transmembrane inner ear (tmie) gene, using the Myo7a promoter, and generated cir/cir homozygous mice carrying the transgene (cir/cir-tgMyo7a) by breeding with circling mice. The cir/cir-tgMyo7a mice still exhibited circling behavior and were unable to swim in water unlike cir/ cir-tgCMV mice and wild-type mice. An auditory brainstem response (ABR) test demonstrated that the cir/cir-tgMyo7a mice could not respond to sound. Immunohistochemical analysis showed enhanced green fluorescent protein, a marker of tmie expression, in the inner and outer hair cells of the cir/cir-tgMyo7a mice. Hair cells and spiral ganglion neurons in the cir/cir-tgMyo7a mice were recovered, but not completely. This study demonstrates that tmie transgene expression in hair cells alone could not restore wild-type hearing and behavior in circling mice.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2017
• Tom: 73
• Numer: 07
• Opis fizyczny: p.399-403,fig.,ref.

Twórcy

autor

Park S.

autor

Jang S.

autor

Lee J.-H.

• Department of Physiology, School of Medicine, University of Nevada, Reno, Nevada, USA

autor

Kim S.;H.

• Center for Laboratory Animal Resources, Kyungpook National University, Daegu, Republic of Korea

autor

Ryoo Z.Y.

• School of Life Sciences, BK21 Plus KNU creative bioresearch group, Kyungpook National University, Daegu, Republic of Korea

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Identyfikatory

DOI

10.21521/mw.5744