Antiviral and cytotoxic activities of anthraquinones isolated from Cassia roxburghii Linn. leaves

• Autorzy: Mohammed M.M.D. , El-Souda S. S. , El-Hallouty S. M. , Kobayashi N.

Warianty tytułu

PL

Aktywność antywirusowa i cytotoksyczna antrachinonów wyizolowanych z liści Cassia roxburghii Linn.

• Języki publikacji: EN

Abstrakty

EN

The cytotoxic activity of petroleum ether extract of the leaves of Cassia roxburghii Linn. against HCT-116 and MCF-7 cell lines resulted with IC50=34.9 and 38.04 μg/ml, respectively, while against HepG-2 showed no activity. A bioassay guided-fractionation approach was conducted to isolate and identify the active cytotoxic principles. Further chromatographic separation and purification of the petroleum ether extract resulted in the isolation of two anthraquinones identified as aloe-emodin acetate and aloe-emodin, along with stigmasterol, β-sitosterol and palmitic acid. The structure elucidation of isolated compounds was performend using 1D, 2D-NMR and HR-MS. Furthermore, the cytotoxicity of aloe-emodin acetate and aloe-emodin were evaluated and resulted with IC50=153.30 and 70.02 μg/ml against HCT-116 and with 93.20 and 53.20 μg/ml against MCF-7, respectively, while against HepG-2 showed no activity. Moreover, the antiviral activity of the two isolated anthraquinones was tested against influenza virus-A, and resulted with IC50=10.23 as well as 2.00 and with CC50=1.32 and 0.47 μg/ml, respectively.

PL

Aktywność cytotoksyczna wyciągu benzynowego z liści Cassia roxburghii Linn. przeciwko liniom komórkowym HCT-116 i MCF-7 wynosiła odpowiednio IC50=34.9 i 38,04 μg/ ml, natomiast nie wykazano aktywności przeciwko HepG-2. Przeprowadzono biologiczne frakcjonowanie w celu wyizolowania i identyfikacji aktywnych związków o działaniu cytotoksycznym. Późniejsza separacja chromatograficzna i oczyszczenie wyciągu benzynowego zaowocowało wyizolowaniem dwóch antrachinonów oznaczonych jako octan aloe-emodyny i aloe-emodyna, a także stigmasterolu, β-sitosterolu i kwasu palmitynowego. Określenie struktury wyizolowanych składników przeprowadzono przy użyciu 1D, 2D-NMR i HR-MS. Określono także cytotoksyczność octanu aloe-emodyny i aloe-emodyny, której wartość wyniosła odpowiednio IC50=153,30 i 70,02 μg/ml przeciwko HCT-116 oraz odpowiednio 93,20 i 53,20 μg/ml przeciwko MCF-7. Nie wykazano aktywności przeciwko HepG-2. Testowano też aktywność przeciwwirusową dwóch wyizolowanych antrachinonów przeciwko wirusowi grypy typu A. Wyniosła ona odpowiednio IC50=10,23 oraz 2,00 przy CC50=1,32 i 0,47 μg/ml.

Słowa kluczowe

EN

antiviral activity; cytotoxic activity; anthraquinone; isolation; Cassia roxburghii; leaf

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2013
• Tom: 59
• Numer: 4
• Opis fizyczny: p.33-44,fig.,ref.

Twórcy

autor

Mohammed M.M.D.

• Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan
• Pharmaceutical and Drug Industries Research Division, Pharmacognosy Department, National Research Center, Dokki-12311, Cairo, Egipt

autor

El-Souda S. S.

• Pharmaceutical and Drug Industries Research Division, Chemistry of Natural Compounds Department, National Research Center, Dokki-12311, Cairo, Egipt

autor

El-Hallouty S. M.

• Pharmaceutical and Drug Industries Research Division, Pharmacognosy Department, National Research Center, Dokki-12311, Cairo, Egipt

autor

Kobayashi N.

• Laboratory of Molecular Biology of Infectious Agents, Graduate School of Biomedical Sciences, School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan

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Identyfikatory

DOI

10.2478/hepo-2013-0022