Validation of procedures based on PCR reactions for detection and identification of genetically modified maize and soybean

• Autorzy: Sieradzki Z. , Mazur M. , Kwiatek K.
• Języki publikacji: EN

Abstrakty

EN

The aim of this study was to validate two procedures for the detection and identification of genetically modified maize (events MON810, Bt11, Bt176 and T25) and soybean (event GTS 40-3-2). Two reference genes for maize genome (invertase) and soya genome (lectin), two GMO screening elements: 35S promoter and NOS terminator, and GM events, characteristic for one of GM crop were the target DNA. The performance criteria taken into account during the study were applicability of CTAB method for DNA extraction and specificity, sensitivity, selectivity, limit of detection, and robustness of PCR reaction. The achieved results proved that the CTAB method with additional purification on QIAquick columns allowed receiving high amount of good quality plant DNA. The optimised for our laboratory conditions PCR reactions reached 100% values of specificity and sensitivity and were able to selectively amplify 0.1% (w/w) of the target DNA in DNA mixture. The obtained results showed that optimised PCR reaction are suitable for the detection and identification of GM maize events MON810, Bt11, Bt176, T25, and GM soybean event GTS 40-3- 2.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2008
• Tom: 52
• Numer: 4
• Opis fizyczny: p.611-614,fig.,ref.

Twórcy

autor

Sieradzki Z.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Mazur M.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

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