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2007 | 29 | 5 |
Tytuł artykułu

Organospecific responses of lupin seedlings to lead. Localization of hydrogen peroxide and peroxidase activity

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In an earlier work using tissue printing method, we found that the PR-10 stress protein was observed in leaf petiole of lupin seedling where lead was not detected (Przymusiński et al. 2001). These results suggested the presence of substance(s) mediating a signal transduction from directly affected cells to distant organs. As the hydrogen peroxide was found to be involved in signal transduction pathway, in the present paper, we analysed the level of H₂O₂ in the organ of lupin seedlings exposed to Pb²⁺ with spectrophotometric method and tissue printing technique. It was unequivocally demonstrated that the level of H₂O₂ and the activity of peroxidase increased in every tested organ of lead-treated lupin seedling. Both the level of H₂O₂ and the activity of POX were correlated with amount of Pb²⁺ ions in the cells (Przymusiński et al. 2001) and decreased in tissues more and more distant from the site of metal application. On the other hand, there was no correlation between the histological localization of H₂O₂ and peroxidase. Our results seem to confirm the hypothesis that H₂O₂ may act as a signalling substance involved in the induction of PR protein synthesis. It was indicated that there is high degree of correlation between the localization of H₂O₂ and the histological localization of PR-10 proteins (Przymusiński et al. 2001) in every tested organ of lupin seedling. The presented hypothesis is also supported by the fact that H₂O₂ and PR-10 proteins are detected in organs and tissues where Pb²⁺ was not found at all.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
29
Numer
5
Opis fizyczny
p.411-416,fig.,ref.
Twórcy
  • Department of Plant Ecophysiology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
  • Department of Plant Ecophysiology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
autor
  • Department of Plant Ecophysiology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
autor
  • Department of Plant Ecophysiology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
Bibliografia
  • Alvarez ME, Pennell RI, Meijer P-J, Ishikawa A, Dixon RA, Lamb C (1998) Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell 92:773–784
  • Antosiewicz DM (2005) Study of calcium-dependent lead-tolerance on plants differing in their level of Ca-deficiency tolerance. Environ Pollut 134:23–34
  • Awade A, Metz-Boutigue MH, Le Ret M, Genot G, Amiri I, Burkard G (1991) The complete amino acid sequence of the pathogenesis-related (PR2) protein induced in chemically stressed bean leaves. Biochim Biophys Acta 1077:241–244
  • Barratt DHP, Clark JA (1991) Proteins arising during the late stages of embryogenesis in Pisum sativum L. Planta 184:14–23
  • Bhattacharjee S (1998) Membrane lipid peroxidation, free radical scavengers and ethylene evolution in Amaranthus as affected by lead and cadmium. Biol Plant 40:131–135
  • Blokhina O, Virolainen E, Fagerstedt KV (2003) Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann Bot 91:179–194
  • Caetano-Anolles G, Gresshoff PM (1991) Efficiency of nodule initiation and autoregulatory responses in a supernodulating soybean mutant. Appl Environ Microbiol 57:2205–2210
  • Capaldi DJ, Taylor KE (1983) A new peroxidase color reaction: oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) with its formaldehyde azine Application to glucose and choline oxidases. Anal Biochem 129:329–336
  • Cassab GI, Varner JE (1987) Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing of nitrocellulose paper. J Cell Biol 105:2581–2588
  • Cassab GI, Varner JE (1989) Tissue printing on nitrocellulose paper: a new method for immunolocalization of proteins, localization of enzyme activities and anatomical analysis. Cell Biol Internat Rep 13:147–152
  • Chen Z, Ricigliano JW, Klessig DF (1993) Purification and characterization of a soluble salicylic acid-binding protein from tobacco. Proc Natl Acad Sci USA 90:9533–9537
  • Cheong YH, Kim CY, Chun HJ, Moon BC, Park HC, Kim JK, Lee SH, Han C-D, Lee SY, Cho MJ (2000) Molecular cloning of a soybean class III β-1,3-glucanase gene that is regulated both developmentally and in response to pathogen infection. Plant Sci 154:71–81
  • Desikan R, Reynolds A, Hancock JT, Neill SJ (1998) Harpin and hydrogen peroxide both initiate programmed cell death but have differential effects on defence gene expression in Arabidopsis suspension cultures. Biochem J 330:115–120
  • Geebelen W, Vangronsveld J, Adriano DC, Van Poucke LC, Clijsters H (2002) Effects of Pb-EDTA and EDTA on oxidative stress reactions and mineral uptake in Phaseolus vulgaris. Physiol Plant 115:377–384
  • Guan LM, Scandalios JG (2000) Hydrogen peroxide-mediated catalase gene expression in response to wounding. Free Rad Biol Med 28:1182–1190
  • Gwóźdź EA, Przymusiński R, Rucińska R, Deckert J (1997) Plant cell responses to heavy metals: ,molecular and physiological aspects. Acta Physiol Plant 19:459–465
  • Hashimoto M, Kisseleva L, Sawa S, Furukawa T, Komatsu S, Koshiba T (2004) A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant Cell Physiol 45:550–559
  • Iturriaga EA, Leech MJ, Barratt DHP, Wang TL (1994) Two ABA-responsive proteins from pea (Pisum sativum L.) are closely related to intracellular pathogenesis-related proteins. Plant Mol Biol 24:235–240
  • Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P (1999) Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science 284:654–657
  • Kopyra M, Gwóźdź EA (2003) Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus. Plant Physiol Bioch 41:1011–1017
  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
  • Levine A, Tenhaken R, Dixon R, Lamb C (1994) H₂O₂ from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell 79:583–593
  • Liu J-J, Ekramoddoullah AKM, Piggott N, Zamani A (2005) Molecular cloning of a pathogen/wound-inducible PR10 promoter from Pinus monticola and characterization in transgenic Arabidopsis plants. Planta 221:159–169
  • Małecka A, Jarmuszkiewicz W, Tomaszewska B (2001) Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Biochim Pol 48:687–698
  • Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
  • Morita S, Kaminaka H, Masumura T, Tanaka K (1999) Induction of rice cytosolic ascorbate peroxidase mRNA by oxidative stress; the involvement of hydrogen peroxide in oxidative stress signalling. Plant Cell Physiol 40:417–422
  • Neill SJ, Desikan R, Clarke A, Hurst RD, Hancock JT (2002) Hydrogen peroxide and nitric oxide as signalling molecules in plants. J Exp Bot 53:1237–1247
  • Olson PD, Varner JE (1993) Hydrogen peroxide and lignification. Plant J 4:887–892
  • Orozco-Cárdenas ML, Narváez-Vásquez J, Ryan CA (2001) Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. Plant Cell 13:179–191
  • Pääkkönen E, Seppänen S, Holopainen T, Kokko H, Kärenlampi S, Kärenlampi L, Kangasjärvi J (1998) Induction of genes for the stress proteins PR-10 and PAL in relation to growth, visible injuries and stomatal conductance in birch (Betula pendula) clones exposed to ozone and/or drought. New Phytol 138:295–305
  • Park H-J, Miura Y, Kawakita K, Yoshioka H, Doke N (1998) Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Plant Cell Physiol 39:1218–1225
  • Przymusiński R, Gwóźdź EA (1999) Heavy metal-induced polypeptides in lupin roots are similar to pathogenesis-related proteins. J Plant Physiol 154:703–708
  • Przymusiński R, Banaszak A, Gwóźdź EA (2001) Organospecific responses of lupin seedlings to lead I. Localization of lead ions and stress proteins. Acta Physiol Plant 23:109–116
  • Przymusiński R, Rucińska R, Gwóźdź EA (2004) Increased accumulation of pathogenesis-related proteins in response of lupine roots to various abiotic stresses. Environ Exp Bot 52:53–61
  • Rucińska R, Waplak S, Gwóźdź EA (1999) Free radical formation and activity of antioxidant enzymes in lupin roots exposed to lead. Plant Physiol Bioch 37:187–194
  • Sarowar S, Young JK, Eui NK, Ki DK, Byung KH, Islam R, Jeong SS (2005) Overexpression of a pepper basic pathogenesis-related protein 1 gene in tobacco plants enhances resistance to heavy metal and pathogen stresses. Plant Cell Rep 24:216–224
  • Srivastava S, Fristensky B, Kav NNV (2004) Constitutive expression of a PR10 protein enhances the germination of Brassica napus under saline conditions. Plant Cell Physiol 45:1320–1324
  • Uchida A, Takabe T, Tkabe T, Jagendorf A (2006) Induction of biosynthesis of osmoprotectants in higher plants by hydrogen peroxide and its application to agriculture. In: Rai AK, Takabe T (eds) Abiotic stress tolerance in plants. Springer, Berlin, pp 153–159
  • Van Camp W, Van Montagu M, Inzé D (1998) H₂O₂ and NO: redox signals in disease resistance. Trends Plant Sci 3:330–334
  • Verma S, Dubey RS (2003) Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants. Plant Sci 164:645–655
  • Vranová E, Inzé D, Van Breusegem F (2002) Signal transduction during oxidative stress. J Exp Bot 53:1227–1236
  • Walter MH, Liu J-W, Grand C, Lamb CJ, Hess D (1990) Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergens. Mol Gen Genet 222:353–360
  • Wang KL-C, Li H, Ecker JR (2002) Ethylene biosynthesis and signaling networks. Plant Cell 14:S131–S151
  • Wierzbicka M (1998) Lead in the apoplast of Allium cepa L root tips— ultrastructural studies. Plant Sci 133:105–109
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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