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2008 | 30 | 6 |
Tytuł artykułu

Purification, characterization and evaluation of insecticidal potential of trypsin inhibitor from mungbean (Vigna radiata L. Wilczek) seeds

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Protease inhibitors present in seeds of legumes possess strong inhibitory activity against trypsin and confer resistance against pests. In the present investigation, trypsin inhibitor activity was found in the seed flour extracts of all the eight selected varieties of mungbean under study which was further confirmed by dot blot analysis. All the varieties showed inhibitory activity in vitro against the gut protease of Helicoverpa armigera (HGP). Trypsin inhibitor was purified from mungbean seeds to near homogeneity with 58.1-fold and 22.8% recovery using heat denaturation, NH4(SO4)2 fractionation, ion-exchange chromatography on DEAE-Sephadex A-25 and gel filtration through Sephadex G-75. The molecular mass of the inhibitor was 47 kDa as determined by gel filtration and SDS-PAGE. The inhibitor retained 90% or more activity between pH 4 and 10, however, it was nearly inactive at extreme pH values. The inhibitor was stable up to 80℃ but thereafter, the activity decreased gradually retaining nearly 30% of activity when heated at 100℃ for 20 min. The inhibitor activity was undetectable at 121℃. Insect bioassay experiment using purified mungbean trypsin inhibitor showed a marked decline in survival (%) of larvae with increase in inhibitor concentration. The larval growth was also extended by the trypsin inhibitor. This study signifies the insecticidal potential of mungbean trypsin inhibitor which might be exploited for raising transgenic plants.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
30
Numer
6
Opis fizyczny
p.761-768,fig.,ref.
Twórcy
autor
  • National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
autor
  • National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
  • National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, India
autor
  • Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India
autor
  • Department of Biochemistry, Kurukshetra University, Kurukshetra, Haryana, India
Bibliografia
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