Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 33 | 3 |
Tytuł artykułu

Effect of newly found polymorphisms in the promoter region of the CAPN1 gene on transcript abundance in broiler chicken breast muscle

Treść / Zawartość
Warianty tytułu
Języki publikacji
The calpain family includes proteolytic enzymes, which have a high capacity to degrade cytoskeletal and muscle fibre proteins. Thus they play an important role in the fusion of myoblasts and in cell proliferation and growth. The CAPN1 gene has been selected as a ‘candidate gene’ for meat quality in many domestic animals, including chickens. Consequently, the aim of our study was to identify new polymorphisms in the promoter region of the CAPN1 gene in broilers and to investigate their impact on CAPN1 transcript abundance in breast muscles. The experiment used broilers of two genetic lines, fast- and slow-growing. Five new polymorphisms in the promoter region of the CAPN1 gene were identified, all of them in linkage disequilibrium (P<0.05). However, the results obtained for their association with expression level were doubtful. Therefore, we surmise that the newly discovered polymorphisms, although they alter the potential sequence binding of transcription factors, probably have just a weak effect on the level of CAPN1 expression in broiler chickens at the investigated stage of ontogenesis.
Słowa kluczowe
Opis fizyczny
  • Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
  • Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
  • Department of Animal Genetics and Breeding, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
  • Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
  • Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland
  • ALLAIS S., JOURNAUX L., LEVÉZIEL H., PAYET-DUPRAT N., RAYNAUD P., HOCQUETTE J.F., LEPETIT J., ROUSSET S., DENOYELLE C., BERNARD-CAPEL C., RENAND G., 2011 – Effects of polymorphisms in the calpastatin and μ-calpain genes on meat tenderness in 3 French beef breeds. Journal of Animal Science 89, 1-11.
  • AZAM M., ANDRABI S.S., SAHR K.E., KAMATH L., KULIPOULOS A., CHISTI A.H., 2001 – Disruption of the mouse μ-calpain gene reveals an essential role in platelet function. Molecular and Cellular Biology 21, 2213-2220.
  • BARRETT L.W., FLETCHER S., WILTON S.D., 2012 – Regulation of eukaryotic gene expression by the untranslated gene regions and other non-coding elements. Cellular and Molecular Life Sciences 69, 3613-3634.
  • BELL D., BERCHUCK A., BIRRER M., CHIEN .J, CRAMER D.W., DAO F., DHIR R., DISAIA P.,2011 – Integrated genomic analyses of ovarian carcinoma. Nature 474, 609-615.
  • BERGER M.F., HODIS E., HEFFERNAN T.P., DERIBE Y.L., LAWRENCE M.S., PROTOPOPOV A., IVANOVA E., WATSON I.R., et al., 2012 – Melanoma genome sequencing reveals frequent PREX2 mutations. Nature 485, 502-506.
  • BILAK S.R., SERNETT S.W., BILAK M.M., BELLIN R.M., STROMER M.H., HUIATT T.W.,ROBSON R.M., 1998 – Properties of the novel intermediate filament protein synemin and its identification in mammalian muscle. Archives of Biochemistry and Biophysics 355, 63-76.
  • BRANCA D., 2004 – Calpain-related diseases. Biochemical and Biophysical Research Communications 322, 1098-1104.
  • CARTHARIUS K., FRECH K., GROTE K., KLOCKE B., HALTMEIER M., KLINGENHOFF A.,FRISCH M., BAYERLEIN M., WERNER T. , 2005 – MatInspector and beyond: promoter analysis based on transcription factor binding sites. Bioinformatics 21, 2933-42.
  • CHEONG H.S., YOON D.H., PARK B.L., KIM L.H., BAE J.S., NAMGOONG S., LEE H.W., HAN C.S., KIM J.O., CHEONG I.C., SHIN H.D., 2008 – A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattle. BMC Genetics 9, 33.
  • CHOMCZYŃSKI P., 1993 – A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15, 532-537.
  • FELÍCIO A.M., BOSCHIERO C., BALIEIRO J.C.C., LEDUR M.C., FERRAZ J.B.S., MICHELAN FILHO T., MOURA A.S.A.M.T., COUTINHO L.L., 2013 – Identification and association of polymorphisms in CAPN1 and CAPN3 candidate genes related to performance and meat quality traits in chickens. Genetics and Molecular Research 12, 472-482.
  • GOLL D.E., THOMPSON V.F, LI H., WEI W., CONG J., 2003 – The calpain system. Physiological Reviews 83, 731-801.
  • HUANG Y., WANG K.K.W., 2001 – The calpain family and human disease. Trends in Molecular Medicine 7, 355-362.
  • IMIELINSKI M., BERGER, A.H., HAMMERMANP.S., HERNANDEZ B., PUGH T.J., HODIS E., CHO J., SUH J., 2012 – Mapping the hallmarks of lung adenocarcinoma with massively parallel sequencing. Cell 150, 1107-1120.
  • KAWABE K., MAEDA Y., OKAMOTO S., HASHIGUCHI T., 1997 – Correlation between skeletal muscle calpain activity and fractional rate of muscle degradation of Japanese quail Coturnix coturnix japonica. Japanese Poultry Science 34, 231-239.
  • L’HONORÉ A., OUIMETTE J.F., LAVERTU-JOLIN M., DROUIN J., 2010 – Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development 137, 3847-3856.
  • LI T.S., LIU F.M., YIN X.H., LIAN A.L., TANG H., QU J.P., 2004 – The evaluation methods of chicken meat tenderness and their relationship. Acta Veterinaria et Zootechnica Sinica 35, 171-177.
  • LIU P.Q., REBAR E.J., ZHANG L., LIU Q., JAMIESON A.C., LIANG Y., QI H., LI P.X., CHEN B.,MENDEL M.C., ZHONG X., LEE Y.L., EISENBERG S.P., SPRATT S.K., CASE C.C., WOLFFE A.P., 2001 – Regulation of an endogenous locus using a panel of designed zinc finger proteins targeted to accessible chromatin regions: Activation of vascular endothelial growth factor A. The Journal of Biological Chemistry 276, 11323-11334.
  • MELODY J.L., LONERGAN S.M., ROWE L.J., HUIATT T.W., MAYES M.S., HUFF-LONERGAN E., 2004 – Early postmortem biochemical factors influence tenderness and water-holding capacity of three porcine muscles. Journal of Animal Science 82, 1195-1205.
  • NCBI, 2013 – Available at
  • ORPHANIDES G., LAGRANGE T., REINBERG D., 1996 – The general transcription factors of RNA polymerase II. Genes and Development 10, 2657-2683.
  • PAGE B.T., CASAS E., HEATON M.P., CULLEN N.G., HYNDMAN D.L., MORRIS C.A.,CRAWFORD A.M., WHEELER T.L., KOOHMARAIE M., KEELE J.W., SMITH T.P.L., 2002 – Evaluation of single-nucleotide polymorphisms in CAPN1 for association with meat tenderness in cattle. Journal of Animal Science 80, 3077-3085.
  • PIÓRKOWSKA K., NOWAK J., POŁTOWICZ K., ROPKA-MOLIK K., 2014 – New polymorphisms in regulatory region of CAPN3 gene with no effect on gene expression in breast muscle of broiler chickens. Annals of Animal Science 14, 511-524.
  • PIÓRKOWSKA K., ROPKA-MOLIK K., ECKERT R., RÓŻYCKI M., 2013 – The expression pattern of proteolytic enzymes of cathepsin family in two important porcine skeletal muscles. Livestock Science 157, 427-434.
  • SINGH L.V., TRIPATHI V., SHARMA R., PANDEY A.K., MAITRA A., MISHRA B.P., 2012 –Genetic polymorphism of CAPN1 gene in Sirohi goat. International Journal of Meat Science 2,13-19.
  • SORIMACHI H., SUZUKI K., 2001 – The structure of calpain. The Journal of Biochemistry 129,653-664.
  • SUN Y., LIU R., LU X., HU Y., ZHAO G., ZHENG M., CHEN J., WANG H., WEN J., 2013 –Associations of polymorphisms in four candidate genes with carcass and/or meat-quality traits in two meat-type chicken lines. Animal Biotechnology 24, 53-65.
  • SUZUKI K., HATA S., KAWABATA Y., SORIMACHI H., 2004 – Structure, activation, and biology of calpain. Diabetes 53(Suppl 1), S12-S18.
  • SUZUKI K., SORIMACHI H., YOSHIZAWA T., KINBARA K., ISHIURA S., 1995 – Calpain: novel family members, activation and physiological function. Biological Chemistry Hoppe-Seyler 376,523-529.
  • YANG X.Q., LIU H., GUO L.J., XU Y., LIU D., 2007 – The mutation site analysis on CAPN1 gene of Wild boar, Min pig and Yorkshire. Hereditas 29, 581-586.
  • YANG X.Q., LIU H., GUO L.J., GUAN Q.Z., XU Y., LIU D., 2008 – Analysis of SNPs in partial exons and 3′UTR of CAPN1 in porcine. Hereditas 30, 741-746.
  • ZHANG Z.R., LIU Y.P., JIANG X., DU H.R., ZHU, Q., 2008 – Study on association of single nucleotide polymorphism of CAPN1 gene with muscle fiber and carcass traits in quality chicken populations. Journal of Animal Breeding and Genetics 125, 258-264.
  • ZHOU G.L,GUO Y., FANG X., WU Y.H., 2011 – Polymorphism of CAPN1 gene and its correlation with meat quality traits in Shouguang chicken. Food Science 32, 207-210.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.