Effect of 3-aminobenzamide on Bcl-2, Bax and AIF localization in hippocampal neurons altered by ischemia reperfusion injury. The immunocytochemical study

• Autorzy: Strosznajder R. , Gajkowska B.
• Języki publikacji: EN

Abstrakty

EN

Poly(ADP-ribose) polymerase plays an important role in cell survival and death. Our previous histological and ultrastructural studies showed that PARP inhibitor 3-aminobenzamide (3-AB) protected neurons against death after ischemia. In this study we investigated the effect of 3-AB on the localization and expression of apoptosis inducing factor (AIF) and on two proteins from Bcl-2 family: Bcl-2 and Bax in hippocampal area CA1, on the 4th day after 3 min of forebrain ischemia in gerbils. Our results indicated that after ischemia AIF is preferentially translocated from the mitochondria to the cytoplasm and to the nucleus. Intravenous administration of 3-AB (30 mg/kg b.w.) prevents AIF translocation to the nucleus. AIF was mainly seen in the structurally unchanged mitochondria and Golgi complex. Moreover, after 3-AB administration overexpression of Bcl-2 protein was observed in mitochondrial membranes, rough endoplasmatic reticulum, Golgi complex, nuclear envelopes, and also in cytoplasm and in nucleus. These data suggest that inhibition of PARP activity may have a beneficial effect on hippocampal neurons through overexpression of Bcl-2 protein and suppression of AIF translocation to the nucleus.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2006
• Tom: 66
• Numer: 1
• Opis fizyczny: p.15-22,fig.,ref.

Twórcy

autor

Strosznajder R.

• Department of Respiratory Research, Polish Academy of Science, 5 Pawinskiego St., 02-106 Warsaw, Poland

autor

Gajkowska B.

• Department of Cell Ultrastructure Medical Research Centre, Polish Academy of Science, 5 Pawinskiego St., 02-106 Warsaw, Poland