Ancylostoma ceylanicum metalloprotease 6 DNA vaccination induces partial protection against hookworm challenge infection

• Autorzy: Wisniewski M. , Jaros S. , Baska P. , Cappello M. , Wedrychowicz H.
• Języki publikacji: EN

Abstrakty

EN

Hookworms are blood feeding intestinal nematodes that infect more than 500 million people and cause iron deficiency anemia. Infected children suffer from physical and cognitive growth retardation. Because of potential anthelminthic drug resistance, the need for vaccine development is urgent. Numerous antigens have been tested in animal models as vaccines against hookworm infection, but there is no effective human vaccine. We cloned a cDNA encoding Ancylostoma ceylanicum metalloprotease 6 (Acemep-6). Ace-MEP-6 is a protein with a predicted molecular mass of 101.87 kDa and based on computational analysis it is very likely to be engaged in food processing via hemoglobin digestion. Groups of hamsters were immunized with an Ace-mep-6 cDNA vaccine, either once or three times. Animals that were administered one dose developed high resistance (80%, p < 0.01) against challenge infection, whereas triple immunization resulted in no worm burden reduction. These results suggest that DNA vaccines can be powerful tools in ancylostomiasis control, although the mechanisms through which protection is conferred remain unclear.

Słowa kluczowe

EN

hookworm; intestinal nematode; infection; Ancylostoma ceylanicum; cDNA encoding; metalloprotease; DNA vaccination

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 3
• Opis fizyczny: p.376-383,fig.,ref.

Twórcy

autor

Wisniewski M.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland

autor

Jaros S.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland

autor

Baska P.

• Division of Pharmacology and Toxicology, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland

autor

Cappello M.

• Program in International Child Health, Departments of Pediatrics, Microbial Pathogenesis and Public Health, Yale University School of Medicine, New Haven, Connecticut 06520-8081, USA

autor

Wedrychowicz H.

• Division of Parasitology and Parasitic Diseases, Department of Preclinical Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, Warsaw, Poland

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