Antibacterial potentials of Vernonia amygdalina against antibiotic-resistant salmonella specie isolated from Nworie River, Imo State, Nigeria

• Autorzy: Chikwendu C.I. , Egbadon E.O. , Okuma B.
• Języki publikacji: EN

Abstrakty

EN

Salmonella species were isolated from Nworie River and then tested against several antibiotics to include; Gentamycin, Streptomycin, Amoxycillin, Kanamycin, Oxacillin, Ofloxacin, Imipenem, Ciprofloxacin, Aztreonam and Ceftazidime. Salmonella isolates obtained from the river were resistant to at least three different antibiotics. All isolates were resistant to Amoxycillin, Oxacillin and Iminipem showing a 100% resistant rate, while showing sensitivity to Ciprofloxacin, Aztreonam, Gentamycin, Ceftazidime, Kanamycin, Streptomycin, and Ofloxacin at sensitivity rates of 71.4%, 7.1%, 77.1%, 11.4%, 44.3%, 48.6% and 78.6% respectively. The antibiotic-resistant Salmonella species were further tested against the aqueous, ethanol and acetone extracts of Vernonia amygdalina leaf, stem and roots to assess their antibacterial potential at a concentration of 100mg/ml. Aqueous extracts of the leaf, stem and roots showed no activity against antibiotic resistant Salmonella isolate, while the ethanol and acetone extracts showed activity rates of 20% and 17% for roots, 14.3% and 12.9% for stem, and, 15.7% and 11.4% for leaf. The results from this study further confirms the antibacterial potentials of Vernonia amygdalina against antibioticresistant bacterial isolates.

Słowa kluczowe

EN

Nigeria; Imo State; Nworie River; river pollution; Vernonia amygdalina; root; bioactive compound; antibacterial potential; antibacterial activity; Salmonella; antibiotic resistance; bacterial infection; medicinal plant

• Wydawca: -
• Czasopismo: International Letters of Natural Sciences
• Rocznik: 2016
• Tom: 56
• Opis fizyczny: p.82-89,ref.

Twórcy

autor

Chikwendu C.I.

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

autor

Egbadon E.O.

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

autor

Okuma B.

• Department of Microbiology, Federal University of Technology, Owerri, Nigeria

Bibliografia

• [1] J. Baudart K. Lemarchand A. Brisabois and P. Lebaron, Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regons. Applied and Environmental Microbiology, 66, (4): (2000) 1544 – 1552
• [2] M.A.N. Medeiros D.C.N. Oliveira D.P. Rodriguez and D.R.C. Freitas, Prevalence and antimicrobial resistance of Salmonella in Chicken carcasses at retail in 15 Brazillian cities. Rev. Panam Salud Publica, 30(6) (2011). 555-560
• [3] V. O. Rotimi W. Jamal T. Pal A. Sonnevend T.S. Dimitrov and M.J. Albert, Emergence of Multi drug resistant Salmonella spp. And isolates with reduced susceptibility to ciprofloxacin in Kuwait and the United Arab Emirates. Diagn. Microbiol.Infect. Dis. 60 (2008) 717
• [4] M. Gordana A. Bogdanka T. Dragica L. Milena and D. Brankica, Antibiotic Susceptibility of Salmonella Spp.: A Comparison Of Two Surveys With A 5 Years Interval, Journal of IMAB – Annual Proceeding (Scientific Papers) 18, (1) (2012) 216-219.
• [5] J. Osterom, Epidemiological studies and proposed preventive measures in the fight against Salmonellosis. Int. J. Food Microbiol. 12 (1999) 41-52
• [6] N.R. Atieno O.P. Owuor and O. Omwoyo, Isolation of High Antibiotic resistant faecal bacteria Indicatiors, Salmonella and Vibro Species from raw Abattoir sewage in Peri-Urban Locations of Nairobi, Kenya. Greener Journal of Biological Sciences, 3(5) (2013) 172-178
• [7] M. Biendo G. Laurans D. Thomas B. Canarelli F. Hamdad-Daoudi F. Rousseau S. Castelain and F. Eb, Molecular characterization and mechanisms of resistance of multidrug-resistant human Salmonella enterica serotype Typhimurium isolated in Amiens (France). Int. J. Antimicrob Agents, 26 (2005) 219-229
• [8] R.S. Seyah J.B. Kaneene Y. Johnson and R.A. Miller, Patterns of antimicrobial resistance observed In Escherichia coli isolates obtained from domestic- and wild-animal fecal samples, human septage, and surface water, Applied Environment Microbiology, 71 (2005) 1394-1404
• [9] A.O. Adebanjo C.O. Adewumi and E.E. Essien, Anti-infection agents from higher plants 5th International Symposium of medicinal plants University of Ife, Nigeria, pp: 152-158 (1983)
• [10] A. Sofowora, Medicinal Plants and Traditional Medicines in Africa. Chichester John Wiley & Sons New York,. pp: 97-145 1993.
• [11] A. O. Ogundare, Antibacterial properties of the leaf extracts of Vernonia amygdalina, Ocimum gratissimum, Corchorous olitorius and Manihot palmate, Journal of Microbiology and Antimicrobials, 3(4) (2011) 77-86
• [12] E.B. Izevbigie, Discovery of water soluble anticancer agents from a vegetable found in Benin City, Nigeria. Exp. Biol. Med., 228 (2003) 293 – 298
• [13] A. Tadesse A. Gebre-Hiwot K. Asres M. Djote D. Frommel, The in-vitro activity of Vernonia amygdalina on Leishmania acthiopica. Ethiop. Med. J., 31 (1993) 183-189.
• [14] R.F. Atata A. Sani and S.M. Ajewole, Effect of stem Stem Bark extracts of Enantia chloranta isolates. Nig. Society of Experimental Biology, 15(2) (2003) 84-92
• [15] B.A. Amadi E.N. Agomuo and C.O. Ibegbulem, Phytochemical test; Research methods in Biochemistry. Supreme Publishers Owerri, pp. 89-95 (2004)
• [16] National Committee for Clinical Laboratory Standard, Method for antimicrobial susceptibility testing. In: Manual of Clinical Microbiology. 5 ed. Washington, D.C. 1105-1125 (1990)
• [17] C. Okore O. Mbanefo B. Onyekwere A. Ugenyi A. Ozurumba U. Nwaehiri and C. Akueshi, Comparative analysis of phytochemical and Antimicrobial effects of extracts of some local herbs on selected pathogenic organism. GRF Davos Planet@Risk, 2(4): 241-248 (2014)
• [18] C.N. Ohalete I.N.S. Dozie I. O. C. Obiajuru and I. H. Eke, Studies on the ecology of Salmonella bacilli in Owerri Metropolis, Imo state Nigeria, Global Research Journal of Science, 1 (2011)109-116
• [19] M. Duru and K. Nwanekwu, Physicochemical and Microbial status of Nworie River, Owerri, Imo state, Nigeria. Asian J. Plant Sci. Res. 2(4) (2012) 433-436
• [20] S.A. Enabuele P.O. Amune and W.T. Aborisade, Antibiograms of Salmonella isolates from poultry farms in Ovia North East Local Govt Area, Edo State, Nigeria. Agric. Biol. J. N. Am. 1(6) (2010) 1287-1290
• [21] A. Mas’ud, and M. Tijani, Prevalent and Multiple antibiotic resistance patterns of Salmonella Serovars isolated from Blood samples of Hospitalised patients in Kano, North-west Nigeria. Journal of Pharmacy and Biological Sciences. 10(2) (2015) 125-135
• [22] O.G. Adeshina N. Osuagwu C.E. Okeke and J.O. Ehinmidu, Plasmid DNA analysis and conjugative study of antibiotic resistant Salmonella typhi and paratyphi to ten selected antibiotics in Zaria, Nigeria. Afr. J. Pharm. Pharmacol. 4(4) (2010) 151-157
• [23] G. O. A. Agada I.O. Abdullahi M. Aminu M. Odugbo, S.C. Chollom P.R. Kumbish and A.E. J. Okwori, Prevalence and Antibiotic Resistance Profile of Salmonella Isolates from Commercial Poultry and Poultry Farm-handlers in Jos, Plateau State, Nigeria. British Microbiology Research Journal 4(4) (2014) 462-479
• [24] T. Suresh A.A. Hatha D. Sreenivasan N. Sangeetha and D. Lashmanaperumalsamy, Prevalence and antimicrobial resistance of Salmonella enteritidis and other Salmonella in the eggs and egg storing trays South India. Journal of Food Microbiology.23 (2006) 294-299.
• [25] O.O. Salami and G.C. Agu, The Assessment of the antimicrobial activities of Ocimum gratissimum (wild basil) and Vernonia amygdalina (Bitter leaf) on some enteric pathogen causing dysentery or diarrhea in patients. IJES 2(9) (2013) 83-96
• [26] A.A. Slayer and D.D. Whiff. Bacteria Pathogens, 1st Edition AMS Press, Washington D.C, pp. 120-240 (1994)
• [27] U. Udochukwu F.I. Omeje I.S. Uloma and F.D. Oseiwe, Phytochemical analysis of Vernonia amygdalina and Ocimum gratissimum extracts and their antibacterial activity on some drug resistant bacteria. American Journal of Research Communication, 3(5) (2015) 225-235
• DOI References
• [1] J. Baudart K. Lemarchand A. Brisabois and P. Lebaron, Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplification of the ribosomal DNA spacer regons. Applied and Environmental Microbiology, 66, (4): (2000). 10.1128/aem.66.4.1544-1552.2000
• [2] M.A.N. Medeiros D.C.N. Oliveira D.P. Rodriguez and D.R.C. Freitas, Prevalence and antimicrobial resistance of Salmonella in Chicken carcasses at retail in 15 Brazillian cities. Rev. Panam Salud Publica, 30(6) (2011). 555-560. 10.1590/s1020-49892011001200010
• [3] V. O. Rotimi W. Jamal T. Pal A. Sonnevend T.S. Dimitrov and M.J. Albert, Emergence of Multi drug resistant Salmonella spp. And isolates with reduced susceptibility to ciprofloxacin in Kuwait and the United Arab Emirates. Diagn. Microbiol. Infect. Dis. 60 (2008). 10.1016/j.diagmicrobio.2007.07.007
• [4] M. Gordana A. Bogdanka T. Dragica L. Milena and D. Brankica, Antibiotic Susceptibility of Salmonella Spp.: A Comparison Of Two Surveys With A 5 Years Interval, Journal of IMAB - Annual Proceeding (Scientific Papers) 18, (1) (2012) 216-219. 10.5272/jimab.2012181.216
• [5] J. Osterom, Epidemiological studies and proposed preventive measures in the fight against Salmonellosis. Int. J. Food Microbiol. 12 (1999) 41-52. 10.1016/0168-1605(91)90046-r
• [6] N.R. Atieno O.P. Owuor and O. Omwoyo, Isolation of High Antibiotic resistant faecal bacteria Indicatiors, Salmonella and Vibro Species from raw Abattoir sewage in Peri-Urban Locations of Nairobi, Kenya. Greener Journal of Biological Sciences, 3(5) (2013). 10.15580/gjbs.2013.5.032113539
• [7] M. Biendo G. Laurans D. Thomas B. Canarelli F. Hamdad-Daoudi F. Rousseau S. Castelain and F. Eb, Molecular characterization and mechanisms of resistance of multidrug-resistant human Salmonella enterica serotype Typhimurium isolated in Amiens (France). Int. J. Antimicrob Agents, 26 (2005). 10.1016/j.ijantimicag.2005.05.003
• [8] R.S. Seyah J.B. Kaneene Y. Johnson and R.A. Miller, Patterns of antimicrobial resistance observed In Escherichia coli isolates obtained from domestic- and wild-animal fecal samples, human septage, and surface water, Applied Environment Microbiology, 71 (2005). 10.1128/aem.71.3.1394-1404.2005
• [20] S.A. Enabuele P.O. Amune and W.T. Aborisade, Antibiograms of Salmonella isolates from poultry farms in Ovia North East Local Govt Area, Edo State, Nigeria. Agric. Biol. J. N. Am. 1(6) (2010) 1287-1290. 10.5251/abjna.2010.1.6.1287.1290
• [23] G. O. A. Agada I.O. Abdullahi M. Aminu M. Odugbo, S.C. Chollom P.R. Kumbish and A.E. J. Okwori, Prevalence and Antibiotic Resistance Profile of Salmonella Isolates from Commercial Poultry and Poultry Farm-handlers in Jos, Plateau State, Nigeria. British Microbiology Research Journal 4(4) (2014). 10.9734/bmrj/2014/5872
• [24] T. Suresh A.A. Hatha D. Sreenivasan N. Sangeetha and D. Lashmanaperumalsamy, Prevalence and antimicrobial resistance of Salmonella enteritidis and other Salmonella in the eggs and egg storing trays South India. Journal of Food Microbiology. 23 (2006). 10.1016/j.fm.2005.04.001

Identyfikatory

DOI

10.18052/www.scipress.com/ILNS.56.82