Peripheral inflammation affects function of trigeminal ganglion neurons

• Autorzy: Kuzawinska O. , Lis K. , Grygorowicz T. , Cudna A. , Dabrowska M. , Mirowska-Guzel D. , Balkowiec-Iskra E.
• Języki publikacji: EN

Abstrakty

EN

BACKGROUND AND AIMS: Our previous studies showed that inflammatory reaction in the area of trigeminal ganglion (TG) nociceptive endings affects neurochemical properties of TG, causing increase in concentration of both brain-derived neurotrophic factor (BDNF) and calcitonin gene-related peptide (CGRP). In the present study we have employed CFA-induced TG inflammation model to begun investigating the underlying molecular mechanisms of the inflammation-induced changes in trigeminal neurons properties. METHODS: A model of orofacial inflammation was obtained by local injection of CFA to the mice whisker pad. Pain reaction was assessed every day by von-Frey filaments. 7 and 14 days after CFA injection mice were euthanized. Both TG were removed for ELISA and quantitative PCR analysis of differences in concentration and expression of BDNF, CGRP and selected proinflammatory cytokines, both in male and female mice. After perfusion with 4% paraformaldehyde, TG were removed from the sculls, crioprotected and cut on the 20 μm sections. Tissue was immunostained using primary antibodies against BDNF and TRPV1 and then with secondary antibodies conjugated with Alexa Fluor. RESULTS: Expression of proinflammatory cytokines, BDNF and CGRP was different in male and female mice. Our results indicate that response of TG to peripheral inflammatory reaction is genderdependent, what may explain differences in frequency and severity of trigeminal nerve-associated disorders observed between women and men. CONCLUSIONS: TG neurons in female mice showed increased expression of CGRPa, this neuropeptide may act as the main mediator of trigeminal signaling during migraine. This project has been supported by the Polish National Science Center, based on the Decision No. DEC-2012/05/B/NZ4/02385. Research subject implemented with CePT infrastructure financed by the European Union – the European Regional Development Fund within the Operational Programme “Innovative economy” for 2007-2013”

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2015
• Tom: 75
• Numer: Supl.
• Opis fizyczny: p.S111

Twórcy

autor

Kuzawinska O.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Lis K.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Grygorowicz T.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Cudna A.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Dabrowska M.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Mirowska-Guzel D.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland

autor

Balkowiec-Iskra E.

• Department of Experimental and Clinical Pharmacology, Medical University of Warsaw, Warsaw, Poland