Nectar defense and hydrogen peroxide in floral nectar of Cucurbita pepo

• Autorzy: Nocentini D. , Guarnieri M. , Soligo C.

Warianty tytułu

PL

Mechanizm obronny oraz nadtlenek wodoru w nektarze kwiatowym Cucurbita pepo

• Języki publikacji: EN

Abstrakty

EN

This study was carried out to investigate some similarities between the nectaries of Nicotiana sp. and Cucurbita pepo, such as starch accumulation in the nectary parenchyma, changes in nectary color during maturation, and the production of a large quantity of sucrose-dominant nectar. The concentration of hydrogen peroxide in C. pepo floral nectar was determined in order to verify the presence of a defense mechanism similar to that found in Nicotiana sp. which protects nectar from yeast and bacteria proliferation. We also tested the eventual accumulation of antioxidants in the nectary of C. pepo as a protection against oxidative stress caused by hydrogen peroxide. The level of hydrogen peroxide found in the floral nectar of C. pepo was much lower than that found in Nicotiana sp. and the male flowers of Cucurbita had a higher concentration than the female flowers. The low oxidative stress induced by this level of hydrogen peroxide caused the accumulation of a low amount of lutein inside the plastoglobules which were contained in amyloplasts. Plastids of the C. pepo nectary are specialized in the accumulation of starch rather than antioxidants.

PL

Przedstawiona praca miała na celu zbadanie niektórych podobieństw pomiędzy nektarnikami Nicotiana sp. i Cucurbita pepo, takich jak gromadzenie skrobi w parenchymie nektarnika, zmiany w kolorze nektarnika w trakcie dojrzewania oraz wytwarzanie dużej ilości nektaru z sacharozą jako cukrem dominującym. Określono stężenie nadtlenku wodoru w nektarze kwiatowym C. pepo w celu sprawdzenia obecności mechanizmu obronnego podobnego do mechanizmu stwierdzonego u Nicotiana sp., który chroni nektar przed rozmnażaniem się drożdży i bakterii. Zbadaliśmy także akumulację przeciwutleniaczy w nektarniku C. pepo jako ochronę przeciwko stresowi oksydacyjnemu spowodowanemu przez nadtlenek wodoru. Poziom nadtlenku wodoru stwierdzony w nektarze kwiatowym C. pepo był o wiele niższy niż u Nicotiana sp., a kwiaty męskie Cucurbita wykazywały wyższe stężenie tego związku niż kwiaty żeńskie. Niewielki stres oksydacyjny wywołany przez ten poziom nadtlenku wodoru powodował gromadzenie się niewielkiej ilości luteiny wewnątrz plastoglobuli, które znajdowały się w amyloplastach. Plastydy nektarnika C. pepo są wyspecjalizowane raczej w gromadzeniu skrobi niż przeciwutleniaczy.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2015
• Tom: 68
• Numer: 2
• Opis fizyczny: p.187-193,fig.,ref.

Twórcy

autor

Nocentini D.

• Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy

autor

Guarnieri M.

• Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy

autor

Soligo C.

• Department of Life Sciences, University of Siena, Via P.A. Mattioli 4, 53100 Siena, Italy

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Identyfikatory

DOI

10.5586/aa.2015.009