Evaluation of the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars diagnosed with mixed mycotoxicosis

• Autorzy: Zielonka L. , Jakimiuk E. , Obremski K. , Gajecka M. , Dabrowski M. , Gajecki M.
• Języki publikacji: EN

Abstrakty

EN

The study evaluated the proliferative activity of immunocompetent cells in the jejunal and iliac lymph nodes of prepubertal female wild boars exposed to deoxynivalenol and zearalenone in naturally contaminated feed. The evaluation was performed with the use of the MTT assay and 2 mitogens: lipopoh saccharide (LPS) and concanavalin A. Intensified proliferative processes in T and B lymphocytes were revealed. The mitogenic activity of LPS was more expressed in the lymphocytes of both iliac and jejunal lymph nodes in comparison with the control group. Proliferative activity was higher in iliac lymph nodes than in jejunal lymph nodes. A reverse trend was observed in the percentage of live cells, which was higher in jejunal lymph nodes during the evaluation of lymphocyte proliferation.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 2
• Opis fizyczny: p.197-203,fig.,ref.

Twórcy

autor

Zielonka L.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Jakimiuk E.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Obremski K.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Dabrowski M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

autor

Gajecki M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, Poland

Bibliografia

• 1. Alassane-Kpembi I., Kolf-Clauw M., Gauthier T., Abrami R., Abiola F.A., Oswald I.P., Puel O.: New insights into mycotoxin mixtures: The toxicity of low doses of type B trichothecenes on intestinal epithelial cells is synergistic. Toxicol Appl Pharm 2013, 272, 191-198.
• 2. Anuszewska E.L.: The research activity of cytotoxic medicinal products and medical devices. Gaz Farm 2010, 5, 36-38.
• 3. Armerding D., Katz D.H.: Activation of T and B lymphocytesin in vitro. 1. Regulatory intluence of bacterial lipopohsaccharide (LPS) on specific T-cell helper function. J Exp Med 1974, 139, 24-43.
• 4. Boermans H.J., Leung M.C.K..: Mycotoxins and the pet food industry: Toxicological evidence and risk assessment. Int J Food Microbiol 2007, 119, 95-102.
• 5. Calabrese E.J.: Paradigm lost, paradigm found: the re-emergence of hormesis as a fundamental dose response model in the toxicological sciences. Environ Pollut 2005, 138, 378-411.
• 6. Cavaliere C., Foglia P., Pastorini E., Samperi R., Laganà A.: Development of a multiresidue method for analysis of major Fusarium mycotoxins in corn meal using liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectr 2005, 19, 2085-2093.
• 7. Davila A.-M., Blachier F., Gotteland M., Andriamihaja M., Benetti P.-H., Sanz Y., Tomé D.: Re-print of "Intestinal luminal nitrogen metabolism: Role of the gut microbiota and consequences for the host". Pharmacol Res 2013, 69, 114-126.
• 8. De Saeger S., van Egmond H.P.: Special issue: masked mycotoxins. World Mycotoxin J 2012, 5, 203-206.
• 9. Dobrzyński L., Fornalski K.W.: Hormesis - Natural phenomenon of answer of organism on stress. Proc VII International Scientific Conference on Veterinary Feed Hygiene - the Effects of Mycotoxins on Gastrointestinal Function. Olszty n, Poland, 2011, pp. 6-14.
• 10. Dwyer J.M., Johnson C.: The use of concanavalin A to study the immunoregulation of human T cells. Clin Exp Immunol 1981, 46, 237-249.
• 11. Frizzell C., Ndossi D., Verhaegen S., Dahl E., Eriksen G., Sřrlie M., Ropstad E., Muller M., Elliott C.T., Connolly L.: Endocrine disrupting effects of zearalenone, alpha- and beta- zearalenol at the level of nuclear receptor binding and steroidogenesis. Toxicol Lett 2011, 206, 210-217...
• 12. Gajęcki M., Gajęcka M., Zielonka L., Jakimiuk E., Obremski K.: Zearalenone as a potential allergen in the alimentary tract - a review. Pol J Food Nutr Sci 2006, 15, 263-268.
• 13. Gajęcka M., Stopa E., Tarasiuk M., Zielonka Ł., Gajęcki M.: The expression of type-1 and tvpe-2 nitric oxide synthase in selected tissues of the gastrointestinal tract during mixed mvcotoxicosis. Toxins 2013, 5, 2281-2292
• 14. Gajęcki M., Zielonka Ł., Obremski K., Jakimiuk E., Gajęcka M.: Multi-mycotoxicosis. Environ Biotechnol 2007, 1, 25-29.
• 15. Gottenberg J.E., Miceli-Richard C., Ducot B., Goupille P., Combe B., Mariette X.: Markers of B-lymphocyte activation are elevated in patients with early rheumatoid arthritis and correlated with disease activity in the ESPOIR cohort. Arthritis Res Ther 2009, 11, R114.
• 16. Grześk E., Grześk G., Koziński M., Stolarek W., Zieliński M., Kubica J.: Nitric oxide as a cause and a potential place therapeutic intervention in hypo responsiveness vascular in early sepsis. Folia Cardiol Excerpta 2011, 6, 36-43.
• 17. Heberer T., Lahrssen-Wiederholt M., Schafft H., Abraham K., Pzyrembel H., Henning K.J., Schauzu M., Braeunig J., Goetz M., Niemann L., Gundert-Remy U., Luch A., Appel B., Banasiak U., Böl G.F., Lampen A., Wittkowski R., Hensel A.: Zero tolerances in food and animal feed - are there any scientific alternatives? A European point of view on an international controversy. Toxicol Lett 2007, 175, 118-135.
• 18. Lun Y., Xia H., Zhang Q., Yu C., Chen N., Li X., Liu S., Lei L.: Anti-inflammatory and immunosuppressive activities of 1,3- dicyclopentyl-1,2,3,6-tetrahydropyrimidine-4,5-dicarboxylic acid diethyl ester (ZL-5015). Int Immunopharmacol 2013, 17, 168-177.
• 19. Maresca M., Fantini J.: Some food-associated mycotoxins as potential risk factors in humans predisposed to chronic intestinal inflammatory diseases. Toxicon 2010, 56, 282-294.
• 20. Maresca M., Yahi N., Younès-Sakr L., Bovron M., Caporiccio B., Fantini J.: Both direct and indirect effects account for the proinflammatory activity of enteropathogenic mycotoxins on the human intestinal epithelium: stimulation of interleukin-8 secretion, potentiation of interleukin-lbeta effect and increase in the transepithelial passage of commensal bacteria. Toxicol Appl Pharmacol 2008, 228, 84-92.
• 21. Obremski K., Gajęcka M., Zielonka L., Jakimiuk E., Gajęcki M.: Morphology and ultrastructure of small intestine mucosa in gilts with zearalenone mycotoxicosis. Pol J Vet Sci 2005, 8, 301-307.
• 22. Obremski K., Zielonka L., Gajęcka M., Jakimiuk E., Bakuła T., Baranowski M., Gajęcki M.: Histological estimation of the small intestine wall after administration of feed containing deoxynivalenol, T-2 toxin and zearalenone in the pig. Pol J Vet Sci 2008, 11, 339-345.
• 23. Pawłowska J., Smoleńska Z., Daca A., Witkowski J.M., Bryl E.: Older age of rheumatoid arthritis onset is associated with higher activation status of peripheral blood CD4+ T cells and disease activity. Clin Exp Immunol 2011, 163, 157-164.
• 24. Przybylska-Gornowicz B., Lewczuk B., Zielonka L., Ziółkowska N., Prusik M., Gajęcki M.: Microscopic structure of duodenum in gilts receiving low doses of zearalenone and deoxynivalenol in diet - preliminary results. Proc 8th International Scientific Conference on Veterinary Feed Hygiene - Undesirable and Desirable Feed Additives. Olsztyn, Poland, 2013, pp. 233-248.
• 25. Silva-Campa E., Mata-Haro V., Mateu E., Hernández J.: Porcine reproductive and respiratory syndrome virus induces CD4⁺ CD8⁺ CD25⁺ regulatory T cells (Tregs). Virology 2012, 430, 73-80.
• 26. Speijers G.J.A., Speijers M.H.M.: Combined toxic effects of mycotoxins. Toxicol Lett 2004, 153, 91-98.
• 27. Vandenberg L.N., Colborn T., Hayes T.B., Heindel J.J., Jacobs D.R., Lee D.-H., Shioda T., Soto A.M., Vom Saal F.S., Welshons W.V., Zoeller R.T., Myers J.P.: Hormones and endocrine-disrupting chemicals: low-dose effects and nonmonotonic dose responses. Endocr Rev 2012, 33, 378-455.
• 28. Wan L.Y.M., Turner P.C., El-Nezamy H.S.: Individual and combined cytotoxic effects of Fusarium toxins (deoxynivalenol, nivalenol, zearalenone and fumonisins Bl) on swine jejunal epithelial cells. Food Chem Toxicol 2013, 57, 276-283.
• 29. Waśkiewicz A., Beszterda M., Kostecki M., Zielonka Ł., Goliński P., Gajęcki M.: Deoxynivalenol in gastrointestinal tract of immature gilts under per os toxin application. Toxins 2014, 6, 973-987.

Identyfikatory

DOI

10.1515/bvip-2015-0030