Ultrastructure of the digestive system and experimental study of feeding in the monogenean skin and fin parasite Macrogyrodactylus congolensis

• Autorzy: Arafa S.Z. , El-Naggar M. M. , Kearn G. C.
• Języki publikacji: EN

Abstrakty

EN

In the present study, transmission electron microscopy (TEM) has been used to study the ultrastructure of the digestive system, namely the pharynx, oesophageal glands and intestine, of the monogenean skin and fin parasite Macrogyrodactylus congolensis. The pharynx consists of an anterior highly muscular region and a posterior mainly glandular syncytial region. The anterior region is provided with six pharyngeal papillae, the centre of each of which is occupied by electron dense secretory bodies, identical with those in the posterior region of the pharynx. The intestine has an uninterrupted syncytial gastrodermis and the luminal surface is provided with many unbranched lamellae. The intestine of living specimens contains large and small granules which give it a reddish brown colour. Large particles, presumed to be lipid droplets, and small granules, presumed to be melanin granules, were found in the gastrodermis and in the intestinal lumen. Parasites were induced to feed and then preserved for TEM at the following intervals: just after feeding, 30 min after feeding, 1 h 30 min after feeding and 2 h after feeding. The specimens were then processed for TEM and sections cut through the intestine of each specimen were examined with the transmission electron microscope. Three types of vacuoles (V1, V2, V3) were detected in the gastrodermis. Vacuoles V1 have thick walls and are likely to be endocytotic, enclosing luminal contents at the surface of the gastrodermis. V2 vacuoles may be lysosomes that fuse with V1 vacuoles. V3 vacuoles may serve to dispose of residual digestive material into the lumen.

Słowa kluczowe

EN

gastrointestinal tract; ultrastructure; experimental study; feeding; digestion; fin; parasite; Macrogyrodactylus congolensis; transmission electron microscopy

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 4
• Opis fizyczny: p.420-433,fig.,ref.

Twórcy

autor

Arafa S.Z.

• Zoology Department, Faculty of Sciences, Mansoura University, Mansoura, Egypt

autor

El-Naggar M. M.

• Zoology Department, Faculty of Sciences, Mansoura University, Mansoura, Egypt

autor

Kearn G. C.

• School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, U.K.

Bibliografia

• Arafa S.Z. 2011. Ultrastructure of musculature of the marginal hooklets of Macrogyrodactylus congolensis, a monogenean skin parasite from the catfish Clarias gariepinus. Acta Parasitologica, 56, 122–130. DOI: 10.2478/s11686-011-0020-3.
• Arafa S.Z., El-Naggar M.M., Kearn G.C. 2003. Scanning electron microscope observations on the monogenean skin parasite Macrogyrodactylus congolensis (Prudhoe, 1957) Yamaguti, 1963. Acta Parasitologica, 48, 163–171.
• Cable J., Harris P.D., Tinsley R.C. 1997. Melanin deposition in the gut of the monogenean Macrogyrodactylus polypteri, Malmberg 1957. International Journal for Parasitology, 27, 1323–1331. DOI: 10.1016/S0020-7519(97)00089-1.
• Cable J., Tinsley R.C., Harris P.D. 2002. Survival, feeding and embryo development of Gyrodactylus gasterostei (Monogenea: Gyrodactylidae). Parasitology, 124, 53–68. DOI: 10.1017/S0031182001008861.
• El-Naggar M.M., Arafa S.Z., El-Abbassy S.A., Kearn G.C. 2001a. Chaetotaxy of the monogeneans Macrogyrodactylus clarii and M. congolensis from the gills and skin of the catfish Clarias gariepinus in Egypt, with a note on argentophilic elements in the nervous system. Folia Parasitologica, 48, 201–208.
• El-Naggar M.M., Arafa S.Z., El-Abbassy S.A., Stewart M.T., Halton D.W. 2007. Neuromusculature of Macrogyrodactylus congolensis, a monogenean skin parasite of the Nile catfish Clarias gariepinus. Parasitology Research, 100, 265–279. DOI: 10.1007/s00436-006-0235-7.
• El-Naggar M.M., El-Naggar A.M., El-Abbassy S.A. 2001b. Microhabitat and movement of the viviparous monogeneans Gyrodactylus alberti, Macrogyrodactylus clarii and M. congolensis from the Nile catfish Clarias gariepinus. Journal of the EgyptianGerman Society of Zoology, Invertebrate Zoology and-Parasitology, 35(D), 169–187.
• El-Naggar M.M., Kearn G.C., Hagras A.E., Arafa S.Z. 1999. On some anatomical features of Macrogyrodactylus congolensis, a viviparous monogenean ectoparasite of the catfish Clarias gariepinus from Nile water. Journal of the Egyptian German Society of Zoology, Invertebrate Zoology and Parasitology, 2(D), 1–24.
• El-Naggar M.M., Serag H.M. 1987. Redescription of Macrogyrodactylus clarii Gussev 1961, a monogenean gill parasite of Clarias lazera in Egypt. Arab Gulf Journal of Scientific Research. Agricultural and Biological Sciences, 5, 257–271.
• Fournier A. 1978. Euzetrema knoepffleri: evidence for a synchronous cycle of gastrodermal activity and an apocrine-like release of the residues of digestion. Parasitology, 77, 19–26. DOI: 10.1017/S0031182000048678.
• Halton D.W. and Stranock, S.D. 1976a. The fine structure and histochemistry of the caecal epithelium of Calicotyle kröyeri (Monogenea: Monopisthocotylea). International Journal for Parasitology, 6, 253–263. DOI: 10.1016/0020-7519(76)90043-6.
• Halton D.W. and Stranock, S.D. 1976b. Ultrastructure of the foregut and associated glands of Calicotyle kroyeri (Monogenea: Monopisthocotylea). International Journal for Parasitology, 6, 517–526. DOI: 10.1016/0020-7519(76)90091-6.
• Halton, D. W. 1975. Intracellular digestion and cellular defecation in a monogenean, Diclidophora merlangi. Parasitology, 70, 331–340. DO: 10.1017/S0031182000052100
• Kearn G.C. 1963. Feeding in some monogenean skin parasites: Entobdella soleae on Solea solea and Acanthocotyle sp. on Raia clavata. Journal of the Marine Biology Association of the United Kingdom, 43, 749–766. DOI: 10.1017/S0025315400025662.
• Kearn G.C. 1979. Studies on gut pigment in skin-parasitic monogeneans, with special reference to the monocotylid Dendromonocotyle kuhlii. International Journal for Parasitology, 9, 545–552. DOI: 10.1016/0020-7519(79)90011-0.
• Kearn G.C., Al-Sehaibani M.A., Whittington I.D., Evans-Gowing, R., Cribb B.W. 1996. Swallowing of sea water and its role in egestion in the monogenean Entobdella soleae, a skin parasite of the common sole (Solea solea), with observations on other monogeneans and on a freshwater temnocephalan. Journal of Natural History, 30, 637–646.
• Khalil, L.F. 1970. Further studies on Macrogyrodactylus polypteri, a monogenean on the African freshwater fish Polypterus senegalus. Journal of Helminthology, 44, 329–348.
• Kritsky D.C., Bourguet D., Spall, R.D. 1994. Fine structure of the gastrodermis of two species of Gyrodactylus (Monogenoidea: Polyonchoinea, Gyrodactylidae). Transactions of the American Microscopical Society, 113, 43–51. DOI: 10.2307/3226578.
• Malmberg G. 1957. On a new genus of viviparous trematodes. Arkiv för Zoologi, 10, 17–330.
• Mo T.A. 1994. Status of Gyrodactylus salaris problems and reseach in Norwy. In: Parasitic Diseases of Fishes (edit by Pike, A. W. and Lewis, J. W.). Samara Publishing Limited, UK.
• Prudhoe, S. 1957. Trematoda. Prac National de l’Upema. I. Mission G. F. et R. Witte en collaboration avec. W. Adam, A. Janssens, L. Van Meet et R. Verheyen (1946–1949), 48, 1–28.
• Sterud E., Harris P.D., Bakke T.A. 1998. The influence of Gyrodactylus salaris Malmberg, 1957 (Monogenea) on the epidermis of Atlantic salmon, Salmo salar L, and brook trout, Salvelinus fontinalis (Mitchill): experimental studies. Journal of Fish Diseases, 21, 257–263. DOI: 10.1046/j.1365-2761.1998.00099.x.
• Tinsley R.C. 1973. Ultrastructure studies on the form and function of the gastrodermis of Protopolystoma xenopi (Monogenoidea: Polyopisthocotylea). Biological Bulletin, 144, 541–555. DOI: 10.2307/1540307.
• Watson N.A., Whittington I.D. 1996. Ultrastructure of the gastrodermis and posterior gut pockets of Concinnocotyla australensis (Platyhelminthes, Monogenea, Polystomatidae) and comparison with another polystomatid, Neopolystoma spratti. Folia Parasitologica, 43, 277–286.