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2018 | 64 | 4 |

Tytuł artykułu

Analysis of scopoletin and mangiferin in botanicals and formulations of Shankhpushpi by HPLC

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Introduction: Shankhpushpi has been widely used in traditional Indian systems of medicine as a brain and memory boosting tonic. There are a variety of botanicals reported to be used as sources of Shankhpushpi in various parts of India. For instance, Canscora decussata Schult, Clitorea ternatea Linn., Convolvulus pluricaulis Choisy. and Evolvulus alsinoides Linn. are most commonly used as sources of Shankhpushpi by practitioners of Ayurveda in different parts of the country. Objective: When it comes to using Shankhpushpi in herbal formulations, qualitative and quantitative analysis of the correct botanicals in the formulation decides its pharmacological effectiveness. Scopoletin and mangiferin are proven bioactive markers identified in Shankhpushpi botanicals in our previous studies. Hence the study is aimed at providing a simple analytical method for the identification of the correct variety of Shankhpushpi using proven markers. Methods: In this study, a High Performance Liquid Chromatographic (HPLC) method has been developed for the estimation of scopoletin and mangiferin levels in four botanicals of Shankhpushpi and their marketed formulations. Result: A simple analytical method was developed which proved to be very crucial in estimating concentrations of mangiferin and scopoletin in various test samples. This method can be used to identify the correct botanicals of Shankhpushpi present in any Ayurvedic formulation or raw material or processed powder by evaluating the content of scopoletin or mangiferin as markers. Conclusion: The developed HPLC method is a quick and reliable method for the quantitative monitoring of mangiferin and scopoletin in herbal extracts and marketed formulations of Shankhpushpi.

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  • Research and Development Manager, Ying Zhi Agricultural and Industries Sdn Bhd, Jitra 06000, Kedah Darulaman, Malaysia
  • Department of Pharmaceutical Sciences, Doctor HS Gour Central University, Sagar-470003, Madhya Pradesh, India


  • Toomula N, Kumar A, Kumar DS, Bheemidi VS. Development and validation of analytical methods for pharmaceuticals. J Anal Bioanal Tech 2011; 2:127. doi:
  • Sethiya NK, Mishra SH. Rapid validated high performance thin layer chromatography method for simultaneous estimation of mangiferin and scopoletin in Canscora decussata (South Indian Shankhpushpi) extract. Revista Brasileira de Farmacognosia 2015; 25:193-198. doi:
  • Parasuraman S, Thing GS, Dhanaraj SA. Polyherbal formulation: Concept of ayurveda. Pharmacogn Rev 2014; 8 (16):73-80. doi:
  • Bhagyasree T, Neelam I, Ajitha A, Rao VUM. A review on analytical method development and validation. Int J Pharm Research Anal 2014; 4(8): 444-448.
  • Archakam SC, Chenchugari S, Banoth CSK. Analytical methods for the recently approved FDA new molecular entities – A review. J Compr Phar 2016; 3(3):70-82.
  • Nahata A, Patil UK, Dixit VK. Effect of Convulvulus pluricaulis Choisy. on learning behaviour and memory enhancement activity in rodents. Nat Prod Res 2008; 22(16):1472-1482. doi:
  • Nahata A, Patil UK, Dixit VK. Anxiolytic activity of Evolvulus alsinoides and Convulvulus pluricaulis in rodents. Pharmaceut Biol 2009; 47(5): 444-451. doi:
  • Sethiya NK, Nahata A, Mishra SH, Dixit VK. An update on Shankhpushpi, a cognition-boosting Ayurvedic medicine. J Chin Integr Med 2009; 7(11):1001-1022.
  • Nahata A, Patil UK, Dixit VK. Effect of Evolvulus alsinoides Linn. on learning behavior and memory enhancement activity in rodents. Phytother Res, 2010; 24:486-493. doi:
  • Sethiya NK, Nahata A, Dixit VK. Comparative thin layer chromatographic investigations on sources of Shankhpushpi. Pharmacog J 2009; 1(3):224-226.
  • Sethiya NK, Mishra SH. Investigation of mangiferin, as a promising natural polyphenol xanthone on multiple targets of Alzheimer’s disease. J Biol Act Prod Nat 2014; 4(2): 111-119. doi:
  • Sethiya NK, Nahata A, Singh PK, Mishra SH. Neuropharmacological evaluation on four traditional herbs used as nervine tonic and commonly available as Shankhpushpi in India. J Ayurveda Integr Med 2017. doi:
  • Krivut BA, Fedyunina NA, Kocherga SI, Rusakoya SV. Spectrophotometric determination of mangiferin. Chem Nat Compd 1976; 12: 36-38. doi:
  • Jubert E, Botha M, Maicu C, De Beer D, Manley M. Rapid screening methods for estimation of mangiferin and xanthone contents of Cyclopia subternata plant material. S Afr J Bot 2012; 82: 113-122. doi:
  • Nahata a, Dixit VK. Spectrofluorimetric estimation of scopoletin in Evolvulus alsinoides Linn. and Convulvulus pluricaulis Choisy. Indian J Pharm Sci 2008; 70(6): 834-837. doi:
  • Sethiya NK, Nahata A, Dixit VK. Simultaneous Spectrofluorimetric determination of scopoletin and mangiferin in a methanolic extract of Canscora decussata Schult. Asian J Trad Med, 2008; 3(6):224-229.
  • Risner CH. The determination of scopoletin in environmental tobacco smoke by high-performance liquid chromatography. J Liq Chromatogr 1994; 17(12): 2723-2736. doi:
  • Suryawanshi S, Asthana RK, Gupta RC. Simultaneous estimation of mangiferin and four secoiridoid glycosides in rat plasma using liquid chromatography tandem mass spectrometry and its application to pharmacokinetic study of herbal preparation. J Chromatogr B: Anal Technol Biomed Life Sci 2007; 858: 211–219. doi:
  • Upadhyay V, Sharma N, Tiwari AK, Joshi HM, Malik A, Singh B, et al. Standardization of HPLC method of scopoletin in different extracts of Convolvulus pluricaulis. Int J Pharm Sci Drug Res 2013; 5: 28-31.
  • Siddiqui MR, Alothman ZA, Rahman N. Analytical techniques in pharmaceutical analysis: A review. Arab J Chem 2017; 10(1):S1409-1421. doi:

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