The effect of 24-epibrassinolide and clotrimazole on the adaptation of Cajanus cajan (L.) Millsp. to salinity

• Autorzy: Dalio R.J.D. , Pinheiro H. P. , Sodek L. , Haddad C. R. B.
• Języki publikacji: EN

Abstrakty

EN

The objective of this study was to evaluate the effects of one of brassinosteroids (24-epibrassinolide) and clotrimazole, (an inhibitor of brassinosteroid synthesis) on plant growth parameters, parameters related to leaf gas exchange (photosynthetic and transpiration rates; stomatal conductance; water use efficiency), photosynthetic pigment content and osmolyte (sugars and proline) content in Cajanus cajan exposed to salinity. Salt stress—caused by NaCl treatment—affected values of all parameters analyzed. The effects were ameliorated by 24-epibrassinolide and intensified by clotrimazole. The hormone increased fresh mass of the plant, shoot dry mass, leaf area, water content of leaves and roots, photosynthetic pigments, sugar concentration, photosynthetic rate, and water use efficiency. The effects of hormone were less evident in the absence of salt. However, under this condition the application of clotrimazole affected the values of parameters studied, indicating the importance of brassinosteroid synthesis for the normal development of the plant.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 5
• Opis fizyczny: p.1887-1896,fig.,ref.

Twórcy

autor

Dalio R.J.D.

• Department of Plant Biology, Institute of Biology, State University of Campinas-UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil

autor

Pinheiro H. P.

• Departament of Statistics, Institute of Mathematics, Statistics and Scientific Computation, University of Campinas-UNICAMP, Campinas, SP, 13083-970, Brazil

autor

Sodek L.

• Department of Plant Biology, Institute of Biology, State University of Campinas-UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil

autor

Haddad C. R. B.

• Department of Plant Biology, Institute of Biology, State University of Campinas-UNICAMP, CP 6109, Campinas, SP, 13083-970, Brazil

Bibliografia

• Akintayo ET, Oshodi AA, Esuoso KO (1999) Effects of NaCl, ionic strength and pH on the foaming and gelation of pigeon pea (Cajanus cajan) protein concentrates. Food Chem 66:51–56
• Ali B, Hayat S, Fariduddin Q, Ahmad A (2008a) 24-Epibrassinolide protects against the stress generated by salinity and nickel in Brassica juncea. Chemosphere 72:1387–1392
• Ali Q, Athar HUR, Ashraf M (2008b) Modulation of growth, photosynthetic capacity and water relations in salt stressed wheat plants by exogenously applied 24-epibrassinolide. Plant Growth Regul 56:107–116
• Amzallag GN, Vaismam J (2006) Influence of brassinosteroids on initiation of the root gravitropic response in Pisum sativum seedlings. Biol Plant 50:283–286
• Ashraf M, Harris PJC (2004) Potential biochemical indicators of salinity tolerance in plants. Plant Sci 166:3–16
• Babita M, Maheswari M, Rao LM, Shanker AK, Rao DG (2010) Osmotic adjustment drought tolerance and yield in castor (Ricinus communis L.) hybrids. Environ Exp Bot 69:243–249
• Bates LS, Waldren RP (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
• Bieleski LR, Turner NA (1966) Separation and estimation of amino acids in crude plant extracts by thin-layer electrophoresis and chromatography. Anal Biochm 17:278–293
• Carillo P, Mastrolonardo G, Nacca F, Fuggi A (2005) Nitrate reductase in durum wheat seedlings as affected by nitrate nutrition and salinity. Funct Plant Biol 32:209–219
• Chen Z, Cuin TA, Zhou M, Twomey A, Naidu BP, Shiabala S (2007) Compatible solute accumulation and stress-mitigating effects in barley genotypes contrasting in their salt tolerance. J Exp Bot 58:4245–4255
• Corzo O, Fuentes A (2004) Moisture sorption isotherms and modeling for pre-cooked flours of pigeon pea (Cajanus cajans L. millsp.) and lima bean (Canavalia ensiformis). J Food Engin 65:443–448
• Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356
• FAO (2000) Global network on integrated soil management for sustainable use of salt affected soils. http://www.fao.org/ag/AGL/agll/spush/intro.htm (accessed 22 December 2008)
• Flowers TJ, Hajibagheri MA (2001) Salinity tolerance in Hordeum vulgare: ion concentrations in root cells of cultivars differing in salt tolerance. Plant Soil 231:1–9
• Fujioka S, Sakurai A (1997) Brassinosteroids. Nat Prod Rep 14:1–10
• Handel EV (1968) Direct microdetermination of sucrose. Anal Biochem 22:280–283
• Hasegawa PM, Bressan RA, Zhu JK, Bohnert HJ (2000) Plant cellular and molecular responses to high salinity. Annu Rev Plant Physiol Plant Mol Biol 51:463–499
• Haubrick LL, Torsethaugen G, Assmann SM (2006) Effect of 24-epibrassinolide, alone and in concert with abscisic acid, on control of stomatal aperture and potassium currents of Vicia faba guard cell protoplasts. Physiol Plant 128:134–143
• Hiscox JD, Israelstam GFA (1979) A method for the extraction of chlorophyll from leaf tissue without maceration. Can J Bot 57:1332–1334
• Hoagland DR, Arnon DI (1938) The water culture method for growing plants without soil. Calif Agric Exp Sta Circ 347
• Janeczko A, Gullner G, Skoczowski A, Dubert F, Barna BE (2007) Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves. Biol Plant 51:355–358
• Kagale S, Divi UK, Krochko JE, Keller WA, Krishna P (2007) Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassinca napus to a range of abiotic stresses. Planta 225:353–364
• Khripach VA, Zhabinskii VN, Groot AE (1999) Brassinosteroids—a new class of plant hormones. Academic Press, San Diego
• Kim TW, Chang SC, Lee JS, Takatsuto S, Yokota T, Kim SK (2004) Novel biosynthetic pathway of castasterone from cholesterol in tomato. Plant Physiol 135:1231–1242
• Kim YS, Joo SH, Hwang JY, Park CH, Kim SK (2006) Characterization of C29-brassinosteroids and their biosynthetic precursors in immature seeds of Phaseolus vulgaris. Bull Korean Chem Soc 27:1117–1118
• Lichtenthaler BK, Welburn AR (1983) Determination of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Transact 11:591–592
• Liska AJ, Shevchenko A, Pick U, Katz A (2004) Enhanced photosynthesis and redox energy production contribute to salinity tolerance in Dunaliella as revealed by homology-based proteomics. Plant Physiol 136:2806–2817
• Lisso J, Altmann T, Mussig C (2006) Metabolic changes in fruits of the tomato d(x) mutant. Phytochemistry 67:2232–2238
• Morillon R, Catterou M, Sangwan RS, Sangwan BS, Lassalles JP (2001) 24-epibrassinolide may control aquaporin activities in Arabidopsis thaliana. Planta 212:199–204
• Nakajima N, Toyama S (1999). Effects of epibrassinolide on sugar transport and allocation to the epicotyl in cucumber seedlings. Plant Prod Sci 2:165–171
• Ogweno JO, Song XS, Shi K, Hu WH, Mao WH, Zhou YH, Yu JQ, Nogués S (2008) Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicum esculentum. J Plant Growth Regul 27:49–57
• Ozdemir F, Bor M, Demiral T, Türkan I (2004) Effects of 24-epibrassinolide on seed germination, seedling growth, lipid peroxidation proline content and antioxidative system of rice (Oryza sativa L.) under salinity stress. Plant Growth Regul 42:203–211
• Paul MJ, Foyer CH (2001) Sink regulation of photosynthesis. J Exp Bot 52:1383–1400
• Reggiani R, Nebuloni M, Mattana M, Brambilla I (2000) Anaerobic accumulation of amino acids in rice roots: role of the glutamine synthetase/glutamate synthase cycle. Amino Acids 18:207–217
• Sasse JM (2003) Physiological actions of brassinosteroids: an update. J Plant Growth Regul 22:276–288
• Shahbaz M, Ashraf M, Athar HUR (2008) Does exogenous application of 24-epibrassinolide ameliorate salt induced growth inhibition in wheat (Triticum aestivum L.)? Plant Growth Regul 55:51–64
• Sharifi M, Ghorbanli M, Ebrahimzadeh H (2007) Improved growth of salinity-stressed soybean after inoculation with salt pre-treated mycorrhizal fungi. J Plant Physiol 164:1144–1151
• Tester M, Davenport R (2003) Na⁺ tolerance and Na ⁺ transport in higher plants. Ann Bot 91:503–527
• Xia XJ, Wang YJ, Zhou YH, Tao Y, Mao WH, Shi K, Asami T, Chen ZX, Yu JQ (2009) Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. Plant Physiol 150:801–814
• Yazici I, Tuerkan I, Sekman AH, Demiral T (2007) Salinity tolerance of purslane (Portulaca oleraceae L.) is achieved by enhanced antioxidative system, lower level of lipid peroxidation and proline accumulation. Environ Exp Bot 61:49–57
• Yu JQ, Huang LF, Hu WH, Zhou YH, Mão WH, Ye SF, Nogués S (2004) A role for brassinosteroid in the regulation of photosynthesis in Cucumis sativus. J Exp Bot 55:1135–1143

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