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2015 | 18 | 4 |
Tytuł artykułu

Influence of chamber type integrated with computer - assisted semen analysis (CASA) system on the results of boar semen evaluation

Autorzy
Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The objective of the study was to evaluate the effect of different types of chambers used in computer-assisted semen analysis (CASA) on boar sperm concentration and motility parameters. CASA measurements were performed on 45 ejaculates by comparing three commonly used chambers: Leja chamber (LJ), Makler chamber (MK) and microscopic slide-coverslip (SL). Concentration results obtained with CASA were verified by manual counting on a Bürker hemocytometer (BH). No significant differences were found between the concentrations determined with BH vs. LJ and SL, whereas higher (p<0.01) values of this parameter were obtained with MK. Compared to MK and SL, significantly higher values were recorded in LJ for velocity (VCL and VAP) as well as amplitude of the lateral head displacement (ALH) and beat cross frequency (BCF), which was associated with significantly higher percentages of motile, progressively motile and rapidly progressive motile spermatozoa. Higher values for the linearity (LIN) and straightness(STR) of sperm movement were obtained for the analysis performed in MK and SL. In both these chambers, the results of all the linearity and kinetic parameters of sperm were similar (p>0.05). The results obtained show that CASA assessment of boar semen should account for the effect of counting chamber on the results of sperm motility and concentration, which confirms the need for further study on standardizing the automatic analysis of boar semen.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
18
Numer
4
Opis fizyczny
p.817-824,ref.
Twórcy
  • Department of Animal Reproduction, Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
Bibliografia
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  • Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM (2012) Application of computer-assisted semen analysis to explain variations in pig fertility. J Anim Sci 90: 779-789.
  • Christensen P, Stryhn H, Hansen C (2005) Discrepancies in the determination of sperm concentration using Bürker-Türk, Thoma and Makler counting chambers. Theriogenology 63: 992-1003.
  • Contri A, Valorz C, Faustini M, Wegher L, Carluccio A (2010) Effect of semen preparation on CASA motility results in cryopreserved bull spermatozoa. Theriogenology 74: 424-435.
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  • Kuster C (2005) Sperm concentration determination between hemacytometric and CASA systems: why they can be different. Theriogenology 64: 614-617.
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  • Massányi P, Chrenek P, Lukáč N, Makarevich AV, Ostro A, Živčak J, Bulla J (2008) Comparison of different evaluation chambers for analysis of rabbit spermatozoa motility parameters using CASA system. Slovak J Anim Sci 41: 60-66.
  • Mortimer ST (2000) CASA – practical aspects. J Androl 21: 515-524.
  • Palacín I, Vincente-Fiel S, Santolaria P, Yániz JL (2013) Standardization of CASA sperm motility assessment in the ram. Small Ruminant Res 112: 128-135.
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  • World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. 5th ed., World Health Organization Press, Geneva.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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