Influence of chamber type integrated with computer - assisted semen analysis (CASA) system on the results of boar semen evaluation
Treść / Zawartość
The objective of the study was to evaluate the effect of different types of chambers used in computer-assisted semen analysis (CASA) on boar sperm concentration and motility parameters. CASA measurements were performed on 45 ejaculates by comparing three commonly used chambers: Leja chamber (LJ), Makler chamber (MK) and microscopic slide-coverslip (SL). Concentration results obtained with CASA were verified by manual counting on a Bürker hemocytometer (BH). No significant differences were found between the concentrations determined with BH vs. LJ and SL, whereas higher (p<0.01) values of this parameter were obtained with MK. Compared to MK and SL, significantly higher values were recorded in LJ for velocity (VCL and VAP) as well as amplitude of the lateral head displacement (ALH) and beat cross frequency (BCF), which was associated with significantly higher percentages of motile, progressively motile and rapidly progressive motile spermatozoa. Higher values for the linearity (LIN) and straightness(STR) of sperm movement were obtained for the analysis performed in MK and SL. In both these chambers, the results of all the linearity and kinetic parameters of sperm were similar (p>0.05). The results obtained show that CASA assessment of boar semen should account for the effect of counting chamber on the results of sperm motility and concentration, which confirms the need for further study on standardizing the automatic analysis of boar semen.
- Department of Animal Reproduction, Biotechnology and Environmental Hygiene, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology, Doktora Judyma 6, 71-466 Szczecin, Poland
- Amann RP, Waberski D (2014) Computer-assisted sperm analysis (CASA): capabilities and potential developments. Theriogenology 81: 5-17.
- Bielański W (1977) Animal Reproduction. 3rd ed., PWRiL, Warsaw.
- Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM (2011) Additional value of computer assisted semen analysis (CASA) compared to conventional motility assessments in pig artificial insemination. Theriogenology 76: 1473-1486.
- Broekhuijse ML, Šoštarić E, Feitsma H, Gadella BM (2012) Application of computer-assisted semen analysis to explain variations in pig fertility. J Anim Sci 90: 779-789.
- Christensen P, Stryhn H, Hansen C (2005) Discrepancies in the determination of sperm concentration using Bürker-Türk, Thoma and Makler counting chambers. Theriogenology 63: 992-1003.
- Contri A, Valorz C, Faustini M, Wegher L, Carluccio A (2010) Effect of semen preparation on CASA motility results in cryopreserved bull spermatozoa. Theriogenology 74: 424-435.
- Didion BA (2008) Computer-assisted semen analysis and its utility for profiling boar semen samples. Theriogenology 70: 1374-1376.
- Douglas-Hamilton DH, Smith NG, Kuster CE, Vermeiden JP, Althouse GC (2005) Capillary-loaded particle fluid dynamics: effect on estimation of sperm concentration. J Androl 26: 115-122.
- Flores E, Fernández-Novell JM, Peña A, Rodríguez-Gil JE (2009) The degree of resistance to freezing-thawing is related to specific changes in the structures of motile sperm subpopulations and mitochondrial activity in boar spermatozoa. Theriogenology 72: 784-797.
- Gadea J (2005) Sperm factors related to in vitro and in vivo porcine fertility. Theriogenology 63: 431-444.
- Gloria A, Carluccio A, Contri A, Wegher L, Valorz C, Robbe D (2013) The effect of the chamber on kinetic results in cryopreserved bull spermatozoa. Andrology 1: 879-885.
- Hoogewijs MK, De Vliegher SP, Govaere JL, De Schauwer C, De Kruif A, Van Soom A (2012) Influence of counting chamber type on CASA outcomes of equine semen analysis. Equine Vet J 44: 542-549.
- Iguer-Ouada M, Verstegen JP (2001) Evaluation of the „Hamilton Thorn computer-based automated system” for dog semen analysis. Theriogenology 55: 733-749.
- Kuster C (2005) Sperm concentration determination between hemacytometric and CASA systems: why they can be different. Theriogenology 64: 614-617.
- Le Lannou D, Griveau JF, Le Pichon JP, Quero JC (1992) Effects of chamber depth on the motion pattern of human spermatozoa in semen or in capacitating medium. Hum Reprod 7: 1417-1421.
- Lenz RW, Kjelland ME, VonderHaar K, Swannack TM, Moreno JF (2011) A comparison of bovine seminal quality assessments using different viewing chambers with a computer-assisted semen analyzer. J Anim Sci 89: 383-388.
- Maes D, Rijsselaere T, Vyt P, Sokolowska A, Deley W, Van Soom A (2010) Comparison of five different methods to assess the concentration of boar semen. Vlaams Diergen Tijds 79: 42-47.
- Massányi P, Chrenek P, Lukáč N, Makarevich AV, Ostro A, Živčak J, Bulla J (2008) Comparison of different evaluation chambers for analysis of rabbit spermatozoa motility parameters using CASA system. Slovak J Anim Sci 41: 60-66.
- Mortimer ST (2000) CASA – practical aspects. J Androl 21: 515-524.
- Palacín I, Vincente-Fiel S, Santolaria P, Yániz JL (2013) Standardization of CASA sperm motility assessment in the ram. Small Ruminant Res 112: 128-135.
- Quintero-Moreno A, Rigau T, Rodríguez-Gil JE (2004) Regression analyses and motile sperm subpopulation structure study as improving tools in boar semen quality analysis. Theriogenology 61: 673-690.
- Rijsselaere T, Van Soom A, Maes D, de Kruif A (2003) Effect of technical settings on canine semen motility parameters measured by the Hamilton-Thorne analyzer. Theriogenology 60: 1553-1568.
- Schmidt H, Kamp G (2004) Induced hyperactivity in boar spermatozoa and its evaluation by computer-assisted sperm analysis. Reproduction 128: 171-179.
- Schulze M, Ruediger K, Mueller K, Jung M, Well C, Reissmann M (2013) Development of an in vitro index to characterize fertilizing capacity of boar ejaculates. Anim Reprod Sci 140: 70-76.
- Verstegen J, Iguer-Ouada M, Onclin K (2002) Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology 57: 149-179.
- World Health Organization (2010) WHO laboratory manual for the examination and processing of human semen. 5th ed., World Health Organization Press, Geneva.