An improved micropropagation protocol for manga bamboo - Pseudoxytenanthera stocksii (Munro) T.Q. Nguyen

• Autorzy: Rajput R.S. , Jani M.D. , Sasikumar J.K. , Manokari M. , Shekhawat M.S.
• Języki publikacji: EN

Abstrakty

EN

The present report illustrates an improved and reproducible micropropagation system for economically valuable bamboo species Pseudoxytenanthera stocksii using nodal segments. Direct organogenesis from nodal segments was accomplished by culturing on Murashige and Skoog (MS) medium supplemented with 6.0 mg/L 6-benzylaminopurine (BAP). On this medium combination 9.0±0.25 shoots per explant were induced. Shoot multiplication was subjective to the combination of auxins and cytokinins concentrations used and number of repeated transfer of mother explants or subculturing of in vitro regenerated shoot clumps on fresh medium. Maximum 41.9±1.00 shoots were observed on MS medium supplemented with 4.0 mg/L of BAP and 0.25 mg/L of naphthalene acetic acid (NAA). Half strength MS medium containing various concentrations of auxins was used for in vitro root formation. Successful rooting with maximum response was achieved on half strength MS medium + 50% sucrose and 1.0 mg/L IBA (24.3±0.27 roots). The rooted plantlets were planted the soilrite and hardened in the greenhouse for 6-8 weeks. Completely acclimatized plantlets exhibited 96% survivability. The present propagation protocol could satisfy the demand of P. stocksii and could be explored for the commercial propagation of this valuable manga bamboo.

• Wydawca: -
• Czasopismo: World News of Natural Sciences
• Rocznik: 2019
• Tom: 25
• Opis fizyczny: p.141-154,fig.,ref.

Twórcy

autor

Rajput R.S.

• Tissue Culture Laboratory, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India
• Prem Sarita AgriBiotech, Rajpipla, Dist. Narmada, Gujarat, India

autor

Jani M.D.

• Tissue Culture Laboratory, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India

autor

Sasikumar J.K.

• Tissue Culture Laboratory, Silviculture and Forest Utilization, Research Range, Rajpipla, Dist. Narmada, Gujarat, India

autor

Manokari M.

• Siddha Medicinal Plants Garden, Central Council for Research in Siddha, Mettur, TN, India

autor

Shekhawat M.S.

• Biotechnology Laboratory, K. M. Centre for P. G. Studies, Puducherry - 605 008, India

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