PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2015 | 64 | 2 |
Tytuł artykułu

Escherichia coli and Salmonella spp. early diagnosis and seasonal monitoring in the sewage treatment process by EMA-qPCR method

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Numerous studies have recently shown that molecular biology tools can allow for early diagnosis of pathogens and can substitute existing cost and time-taking traditional methods. One of them, the qPCR, is successfully used in microbiology and its utility has been assessed for many different biological materials. The aim of this study was to: 1) determine, optimize and apply qPCR as a method to detect Escherichia coli and Salmonella spp. in primary influents and final effluents from municipal wastewater treatment plant 2) define if addition of ethidium bromide monoazide (EMA) before DNA extraction can allow to distinguish between alive and dead bacteria, 3) quantify E.coli and Salmonella spp. in wastewater during four seasons by qPCR and traditional spread plate method and determine the correlation between the indicator and pathogenic microorganisms. The obtained results has shown that qPCR can be used as a quantitative method in the diagnosis of investigated bacteria in wastewater with EMA pretreatment as a crucial step for a proper quantitative analysis of the presence of these bacteria in wastewater. Both E.coli and Salmonella spp. bacteria species were present in all samples of primary influents and final effluents. Our study shown that the quantity of investigated bacteria is strictly correlated with the season that they were obtained in.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
64
Numer
2
Opis fizyczny
p.p.143-148,fig.,ref.
Twórcy
autor
  • Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland
  • Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland
autor
  • Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland
autor
  • Institute of Environmental Engineering, Czestochowa University of Technology, Czestochowa, Poland
autor
Bibliografia
  • Besser R.E., S.M. Lett., J.T. Weber, M.P. Doyle, T.J. Barrett, J.G. Wells and P.M. Griffin. 1993. An outbreak of diarrhea and hemolytic uremic syndrome from Escherichia coli O157:H7 in fresh-pressed apple cider. JAMA 269: 2217–2220.
  • Calvó L., A, Martínez-Planells, J. Pardos-Bosch and L.J. Garcia-Gi. 2008. A New Real-Time PCR Assay for the Specific Detection of Salmonella spp. targeting the bipA gene. Food Anal. Method. 4: 236–242.
  • Fijałkowski K., Kacprzak M. and A. Rorat. 2014. Occurrence changes of Escherichia coli (including O157:H7 serotype) in wastewater and sewage sludge by quantitation method of (EMA) real time-PCR. Desalin. Water Treat. 52: 1-8.
  • FDA. U.S. Food and Drug Administration. 2003. Foodborne Pathogenic Microrganisms and Natural Toxins Handbook – Salmonella spp.
  • Gedalanga P.B. and B.H. Olson. 2009. Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples. Appl. Microbiol. Biot. 82: 587–596.
  • Gilbride K.A., D.Y. Lee and L.A. Beaudette. 2006. Molecular techniques in wastewater: Understanding microbial communities. detecting pathogens and real-time process control. J. Microbiol. Methods 66: 1–20.
  • Giovannoni S.J., T. B. Britschgi, C.L. Moyer and K.G. Field. 1990. Genetic diversity in Sargasso Sea bacterioplankton. Nature 344: 60–62.
  • Hagendorf U., K. Diehl, I. Feuerpfeil, A. Hummel, J. Lopez-Pila and R. Szewzyk. 2004 Microbiological investigations for sanitary assessment of wastewater treated in constructed wetlands. Water Res. 39: 4849–4858.
  • Lee D.Y., K. Shannon and L.A. Beaudette. 2006. Detection of bacterial pathogens in municipal wastewater using an oligonucleotide microarray and real-time quantitative PCR. J. Microbiol. Methods 65: 453–467.
  • Morio F., S. Corvec, N. Caroff, F. Le Gallou, H. Drugeon and A. Reynaud. 2008. Real-time PCR assay for the detection and quantification of Legionella pneumophila in environmental water samples: Utility for daily practice. Int. J. Hyg. Envir. Heal. 211: 403–411.
  • Nocker A. and A.K. Camper. 2006. Selective removal of DNA from dead cells of mixed bacterial communities by use of ethidium monoazide. Appl. Environ. Microb. 72: 1997–2004.
  • Nogva H.K, S.M. Dromtorp, H. Nissen and K. Rudi. 2003. Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5-Nuclease PCR. BioTechniques 34: 804–813.
  • Patra A.K., B.C.Acharya and A. Mohapatra. 2009. Occurrence and distribution of bacterial indicators and pathogens in coastal waters of Orissa. Indian J. Mar. Sci. 38: 474–480.
  • Rudi K., K. Naterstad, S.M. Dromtorp and H. Holo. 2005. Detection of viable and dead Listeria monocytogenes on gouda-like cheeses by real-time PCR. Lett. Appl. Microbiol. 40: 301–306.
  • Sahlstrom L., A. Aspan, E. Bagge, M.L. Danielsson-Tham and A. Albihn. 2004. Bacterial pathogen incidences in sludge from Swedish sewage treatment plants. Water Res. 38: 1989–1994.
  • Shannon K.E., D.Y. Lee, J.T. Trevors and L.A. Beaudette. 2007. Detection of bacterial pathogens during wastewater treatment using real-time PCR. Sci. Total Environ. 282: 121–129.
  • Sidhu J.P.S and S.G. Toze. 2009. Human pathogens and their indicators in biosolids: A literature review. Environ. Int. 35: 187–201.
  • Soejima T., K. Iida, T. Qin, H. Taniai, M. Seki and S. Yoshida. 2008. Method to detect only live bacteria during PCR amplification. J. Clin. Microbiol. 46: 2305–2313.
  • Song Z., Q. Sun, M. Yu, Y. Zhou, X. Kong and Y. Zhao. 2010. Seasonal variations and correlation of Escherichia coli and Salmonellae in a full-scale constructed wetland for wastewater treatment in China. Proceedings of 4th International Conference of Bioinformatics and Biomedical Engineering (ICBBE). 18–20 June 2010, Chengdu.
  • Ulrich H., D. Klaus, F. Irmgard, H. Annette, L.P. Juan and S. Regine. 2005. Microbiological investigations for sanitary assessment of wastewater treated in constructed wetlands. Water Res. 39: 4849–4858.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-cce72a58-d61c-4f6a-81bf-72181c6733b2
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.