Statistical analysis of the associations between phenolic monoterpenes and molecular markers, AFLPs and SAMPLs in the spice plant Oregano

• Autorzy: Azizi A. , Ardalani H. , Honermeier B.

Warianty tytułu

PL

Analiza statysyyczna związku monoterpenów fenolowych i markerów molekularnych AFLP I SAMPL Lebiodki pospolitej (Oregano)

• Języki publikacji: EN

Abstrakty

EN

Introduction: Molecular markers are the examples of the contribution of genome technology to medicinal plant breeding through marker-assisted selection (MAS) for pharmaceutical quality. Objective: Forty-two accessions of Origanum vulgare L. originating from Europe were evaluated to detect genomic and chemotypic polymorphisms and to discover possible associations between them. Methods: A total of 477 molecular polymorphisms including 214 AFLP (Amplified Fragment Length Polymorphism) and 263 SAMPL (Selectively Amplified Microsatellite Polymorphic Loci) were used for genotyping. Components in the essential oils were identified and quantified by gas chromatography (GC) and two major compounds (two economically important monoterpenes: carvacrol and thymol) were investigated. Results: Based on results, a relatively high correlation between chemotypic patterns and genetic markers was identified. Associations between traits of interest for essential oils (carvacrol and thymol content) and genetic markers were tested using five statistical methods including three General Linear Model (GLM) and two unified Mixed Linear Model (MLM) approaches. Significant associations were found for 3 AFLP and 20 SAMPL with three key traits including essential oil yield, carvacrol and thymol content. Conclusion: These associations can constitute a useful starting point for marker-assisted selection. Therefore, the results provide the basis for molecular breeding of O. vulgare for pharmaceutical purposes.

PL

Wstęp: Markery molekularne stanowią przykład udziału technologii genomowej w hodowli roślin leczniczych za pomocą selekcji typu MAS, prowadzonej na potrzeby przemysłu farmaceutycznego. Cel: Zbadano 42 rośliny Origanum vulgare L. pochodzące z Europy w celu wykrycia zmienności genomu i chemotypu oraz określenia potencjalnych zależności między nimi. Metody: Oceniono 477 polimorfizmów molekularnych stosując technikę AFLP (Amplified Fragment Length Polymorphism) dla 214 polimorfizmów i metodę SAMPL (Selectively Amplified Microsatellite Polymorphic Loci) dla 263 polimorfizmów. Za pomocą chromatografii gazowej (GC) zidentyfikowano i określono składniki olejków eterycznych oraz zbadano ich dwa główne komponenty, monoterpeny o znaczeniu ekonomicznym: karwakrol i tymol). Wyniki: Na podstawie uzyskanych wyników stwierdzono stosunkowo wysoką korelację pomiędzy wzorem chemotypowym a markerami genetycznymi. Związek między badanymi cechami olejków eterycznych (zawartością karwakrolu i tymolu) a markerami genetycznymi oceniono za pomocą pięciu metod statystycznych stosując trzy ogólne modele liniowe (General Linear Model – GLM) i dwa liniowe modele mieszane (Mixed Linear Model – MLM). Wykazano istotne zależności między markerami genetycznymi (3 AFLP i 20 SAMPL) a trzema kluczowymi cechami surowca: zawartością olejku eterycznego, karwakrolu i tymolu. Wnioski: Stwierdzone zależności mogą wskazywać na przydatność badań molekularnych w hodowli roślin. Uzyskane wyniki stanowią podstawę dla zastosowania markerów molekularnych w selekcji O. vulgare do celów farmaceutycznych.

• Wydawca: -
• Czasopismo: Herba Polonica
• Rocznik: 2016
• Tom: 62
• Numer: 2
• Opis fizyczny: p.42-56,fig.,ref.

Twórcy

autor

Azizi A.

• Department of Horticultural Sciences, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

autor

Ardalani H.

• Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

autor

Honermeier B.

• Institute of Agronomy and Plant Breeding, Biomedical Research Centre Seltersberg (BFS), Justus Liebig University Giessen, Schubert-Str.81, D-35392 Giessen, Germany

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Identyfikatory

DOI

10.1515/hepo-2016-0010