Optimisation of protocol for Clostridium botulinum detection in mink feed

• Autorzy: Grenda T. , Kukier E. , Goldsztejn M. , Kwiatek K. , Koziel N.
• Języki publikacji: EN

Abstrakty

EN

As the test material mink feed with natural microflora was used. The analyses were conducted using Wrzosek and TPGY broth media, and Willis-Hobbs and Zeissler differential agar media. Wrzosek, Willis-Hobbs, and Zeissler media are described in Polish Standards approved by the National Standards Body in Poland and routinely used in detection of anaerobic bacteria in Poland. Detection and identification of C. botulinum was performed with a previously validated real-time PCR method based on ntnh gene detection, which is common in all C. botulinum toxotypes. The use of Wrzosek broth and Zeissler agar in routine analyses for detection and identification of C. botulinum was ineffective and limited. The obtained results showed the highest culturing process effectiveness in TPGY broth with 72 h incubation at 30°C and isolation on Willis-Hobbs agar. The real-time PCR method based on ntnh gene detection used in this study could be utilised as a supplementary tool to the mouse lethality assay.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2015
• Tom: 59
• Numer: 3
• Opis fizyczny: p.377-382,fig.,ref.

Twórcy

autor

Grenda T.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kukier E.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Goldsztejn M.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kwiatek K.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Koziel N.

• Department of Hygiene of Animal Feedingstuffs, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

• 1. Anniballi F., Fiore A., Löfström C., Skarin H., Auricchio B., Woudstra C., Bano L., Segerman B., Koene M., Båverud V., Hansen T., Fach P., Åberg A.T., Hedeland M., Engvall E.O., De Medici D.: Management of animal botulism outbreaks: from clinical suspicion to practical countermeasures to prevent or minimize outbreaks. Biosecur Bioterror 2013, 11, 191-199.
• 2. Anon.: Animal feeding stuffs - criteria and microbiological examinations. PN-R-64791:1994.
• 3. Anon.: Commission Regulation (EU)No 142/2011 implementing Regulation (EC) No 1069/2009 of the European Parliament and of the Council laying down health rules as regards animal byproducts and derived products not intended for human consumption and implementing Council Directive 97/78/EC as regards certain samples and items exempt from veterinary checks at the border under that Directive.
• 4. Anon.: Meat and meat products. Microbiological examinations. Detection of anaerobic spore-forming bacteria and anaerobic spore-forming bacteria of sulphite (IV) reducing. PN-A-82055- 12:1997.
• 5. Anon.: Microbiology of food and animal feeding stuffs. Protocol for the validation of alternative methods. EN ISO 16140:2003.
• 6. Anon.: Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. EN ISO 6579:2002.
• 7. Anon.: Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count method. EN ISO 21528-2:2004.
• 8. Anon.: Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase- positive Escherichia coli - Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. EN ISO 16649-2:2001.
• 9. Anon.: Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of presumptive Bacillus cereus - colony-count technique at 30 degrees C. EN ISO 7932:2004.
• 10. Anon.: Regulation (EC) No 1069/2009 of the European Parliament and of the Council laying down health rules as regards animal by-products and derived products not intended for human consumption and repealing Regulation (EC) No 1774/2002 (Animal by-products Regulation).
• 11. Anon.: Microbiology of food and animal feeding stuffs - horizontal method for the enumeration of Clostridium perfringens - colony-count technique. EN ISO 7937:2004.
• 12. Barash J.R., Arnon S.S.: A novel strain of Clostridium botulinum that produces type B and type H botulinum toxins. J Infect Dis 2013, doi: 10.1093/infdis/jit449.
• 13. Cato E.P., George W.L., Finegold S.M.: Genus Clostridium, Prazmowski, 1880, In: G. Bergey's Manual of Systematic Bacteriology 2, edited by Sneath P.H.A., Mair N.S., Sharpe M.E., Holt J., Williams and Wilkins, Baltimore 1986, pp. 1141-1200.
• 14. Cunniff P.: Official Methods of Analysis of AOAC International. AOAC Int Inc 1995, 17, 46-48.
• 15. Dahlenborg M., Borch E., Radström P.: Development of a combined selection and enrichment PCR procedure for C. botulinum types B, E, and F and its use to determine prevalence in fecal samples from slaughtered pigs. Appl Environ Microbiol 2001, 67, 4781-4788.
• 16. Dahlenborg M., Borch E., Radström P., Prevalence of C. botulinum types B, E and F in faecal samples from Swedish cattle. Int J Food Microbiol 2003, 82, 105-110.
• 17. Fletcher G.C., Youssef J.F., Lu G.: Selecting methods for determining the presence of BoNT genes in New Zealand marine sediments. Crop & Food Research Confidential Report. New Zealand, 2008.
• 18. Grenda T., Kwiatek K.: Clostridium botulinum - characteristic features and epidemiological significance. Med Weter 2009, 65, 743-746,.
• 19. Grenda T., Kwiatek K.: In house validation of real-time PCR method for detection of Clostridium botulinum in food and feed matrixes. Bull Vet Inst Pulawy 2010, 54, 557-562.
• 20. Gryko R., Goszczyński D., Lorkiewicz Z.: A simple method of detection of toxigenic Clostridium botulinum type B strains. J Microbiol Meth 1990, 11, 187-193.
• 21. Hall I.C., Stiles G.W.: An outbreak of botulism in captive mink on a fur farm in Colorado. J Bacteriol 1938, 36, 282.
• 22. Hielm S., Hyytia E., Ridell J., Korkeala H.: Detection of C. botulinum in fish and environmental samples using polymerase chain reaction. Int J Food Microbiol 1996, 31, 357-365.
• 23. Hyytiä E., Hielm S., Korkeala H.: Prevalence of C. botulinum type E in Finnish fish and fishery products. Epidemiol Infect 1998, 120, 245-250.
• 24. Lindström M., Korkeala H.: Laboratory diagnostics of botulism. Clin Microbiol Rev 2006, 19, 298-314.
• 25. Lindström M., Nevas M., Kurki J., Sauna-aho R., Latvala- Kiesilä A., Pölönen I., Korkeala H.: Type C botulism due to toxic feed affecting 52,000 farmed foxes and minks in Finland. J Clin Microbiol 2004, 42, 4718-4725.
• 26. Myllykoski J., Lindström M., Bekema E., Pölönen I., Korkeala H.: Fur animal botulism hazard due to feed. Res Vet Sci 2011, 90, 412-418.
• 27. Pospiech E., Frankowska A.: Nitrates III and V - their application and future in food processing Med Weter 2009, 65, 803-806.
• 28. Raphael B.H., Anreadis J.D.: Real-time PCR detection of the nontoxic nonhemagglutinin gene as a rapid screening method for bacterial isolates harboring the botulinum neurotoxin (A-G) gene complex. J Microbiol Meth 2007, 71, 343-346.
• 29. Saeed E.M.A.: Studies on isolation and identification of Clostridium botulinum investigating field samples especially from equine grass sickness cases. Thesis, Faculty of Agriculture, Goettingen University, Goettingen, 2004.

Identyfikatory

DOI

10.1515/bvip-2015-0056