Several factors affecting hypericin production of Hypericum perforatum during adventitious root culture in airlift bioreactors

• Autorzy: Wu S.-Q. , Yu X.-K. , Lian M.-L. , Park S.-Y. , Piao X.-C.
• Języki publikacji: EN

Abstrakty

EN

Hypericum perforatum L. is a traditional medicinal plant for the treatment of depression and wound healing, and hypericin is one of the main effective active substances. To optimize the culture system for producing hypericin in adventitious root, this study used balloon-type airlift bioreactors to investigate the effect of air volume, inoculation density, indole-3-butyric acid (IBA) concentration and methyl jasmonate (MeJA) concentration on hypericin content and productivity during adventitious root culture. Hypericin content and productivity were improved with increasing air volume, and 0.1 vvm (air volume/culture volume/min) was optimal for hypericin production. Inoculation density also had a great effect on hypericin accumulation. Hypericin content and productivity were favorable in an inoculation density of 5.0 g l⁻¹ and decreased when inoculation densities were lower or higher than 5.0 g l⁻¹. Furthermore, 1.25 mg l⁻¹ IBA enhanced hypericin content and productivity, but too low (≤0.50 mg l⁻¹) or too high (≥1.50 mg l⁻¹) IBA concentrations decreased hypericin accumulation. MeJA concentration significantly affected biomass accumulation and hypericin production. The biomass decreased and hypericin production increased with increasing MeJA concentration. Optimum hypericin content (1.61 mg g⁻¹ DW) and productivity (15.57 mg l⁻¹) were obtained at 350 μM MeJA. The hypericin content in bioreactor-grown adventitious roots was lower than in 3-year field-grown plants, but significantly higher than that in in vitro-grown plantlets and 1-year field-grown plants. Thus, the bioreactor culture of adventitious roots can realize rapid and mass production of hypericin in H. perforatum.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 04
• Opis fizyczny: p.975-981,fig.,ref.

Twórcy

autor

Wu S.-Q.

• Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, 133002 Yanji, Jilin, China

autor

Yu X.-K.

• Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, 133002 Yanji, Jilin, China

autor

Lian M.-L.

• Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, 133002 Yanji, Jilin, China

autor

Park S.-Y.

• Department of Horticulture Science, Chungbuk National University, 361-763 Cheongju, Republic of Korea

autor

Piao X.-C.

• Key Laboratory of Nature Resource of Changbai Mountain and Functional Molecular, Yanbian University, Ministry of Education, Park Road 977, 133002 Yanji, Jilin, China

Bibliografia

• Ahmed S, Hahn EJ, Paek KY (2008) Aeration volume and photosynthetic photon flux affect cell growth and secondary metabolite contents in bioreactor cultures of Morinda citrifolia. J Plant Biol 51:209–212
• Bagdonaite E, Mártonfi P, Repčák M, Labokas J (2010) Variation in the contents of pseudohypericin and hypericin in Hypericum perforatum from Lithuania. Biochem Syst Ecol 38:634–640
• Baque MA, Hahn EJ, Paek KY (2010) Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin. Plant Biotechnol Rep 4:109–116
• Büter B, Orlacchio C, Soldati A, Berger K (1998) Significance of genetic and environmental aspects in the field cultivation of Hypericum perforatum. Planta Med Nat Prod Med Plant Res 64:431–437
• Butterweck V (2003) Mechanism of action of St John’s wort in depression: what is known? CNS Drugs 17:539–562
• Chakrabarty D, Hahn EJ, Yoon YJ, Paek KY (2003) Micropropagation of apple rootstock M.9 EMLA using bioreactor. J Hortic Sci Biotechnol 78:605–609
• Chen LR, Chen YJ, Lee CY, Lin TY (2007) MeJA-induced transcriptional changes in adventitious roots of Bupleurum kaoi. Plant Sci 173:12–24
• Cui XH, Chakrabarty D, Lee EJ, Paek KY (2010) Production of adventitious roots and secondary metabolites by Hypericum perforatum L. in a bioreactor. Biores Technol 101:4708–4716
• Cui XH, Murthy HN, Jin YX, Yim YH, Kim JY, Paek KY (2011) Production of adventitious root biomass and secondary metabolites of Hypericum perforatum L. in a balloon type airlift reactor. Biores Technol 102:10072–10079
• Donnez D, Kim K-H, Antoine S, Conreux A, De Luca V, Jeandet P, Clement C, Courot E (2011) Bioproduction of resveratrol and viniferins by an elicited grapevine cell culture in a 2 L stirred bioreactor. Proc Biochem 46:1056–1062
• Hadjur C, Richard MJ, Parat MO, Jardon P, Favier A (2008) Photodynamic effects of hypericin on lipid peroxidation and antioxidant status in melanoma cells. Photochem Photobiol 64:375–381
• Jeong JA, Wu CH, Murthy HN, Hahn EJ, Paek KY (2009) Application of an airlift bioreactor system for the production of adventitious root biomass and caffeic acid derivatives of Echinacea purpurea. Biotechnol Bioproc Eng 14:91–98
• Kirakosyan A, Hayashi H, Inoue K, Charchoglyan A, Vardapetyan H (2000) Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures. Phytochemistry 53:345–348
• Kornfeld A, Kaufman PB, Lu CR, Gibson DM, Bolling SF, Warber SL, Chang SC, Kirakosyan A (2007) The production of hypericins in two selected Hypericum perforatum shoot cultures is related to differences in black gland structure. Plant Physiol Biochem 45:24–32
• Lawvere S, Mahoney MC (2005) St. John’s wort. Am Fam Phys 72:2249
• Lee EJ, Mobin M, Hahn EJ, Paek KY (2006) Effects of sucrose, inoculum density, auxins, and aeration volume on cell growth of Gymnema sylvestre. J Plant Biol 49:427–431
• Lian ML, Chakrabarty D, Paek KY (2002) Effect of plant growth regulators and medium composition on cell growth and saponin production during cell-suspension culture of mountain ginseng (Panax ginseng C.A. Mayer). J Plant Biol 45:201–206
• Linde K (2009) St. John’s wort-an overview. Forsch Komplementärmed/Res Complem Med 16:146–155
• Meruelo D, Lavie G, Lavie D (1988) Therapeutic agents with dramatic antiretroviral activity and little toxicity at effective doses: aromatic polycyclic diones hypericin and pseudohypericin. Proc Nat Acad Sci 85:5230–5234
• Min JY, Jung HY, Kang SM, Kim YD, Kang YM, Park DJ, Prasad DT, Choi MS (2007) Production of tropane alkaloids by smallscale bubble column bioreactor cultures of Scopolia parviflora adventitious roots. Biores Technol 98:1748–1753
• Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
• Murthy HN, Hahn EJ, Paek KY (2008) Adventitious roots and secondary metabolism. Chin J Biotechnol 24:711–716
• Schlatmann JE, Nuutila AM, Van Gulik WM, Ten Hoopen HJG, Verpoorte R, Heijnen JJ (2004) Scaleup of ajmalicine production by plant cell cultures of Catharanthus roseus. Biotechnol Bioeng 41:253–262
• Shohael AM, Murthy HN, Hahn EJ, Lee HL, Paek KY (2008) Increased eleutheroside production in Eleutherococcus sessiliflorus embryogenic suspension cultures with methyl jasmonate treatment. Biochem Eng J 38:270–273
• Sirvent T, Gibson D (2002) Induction of hypericins and hyperforin in Hypericum perforatum L. in response to biotic and chemical elicitors. Physiol Mol Plant Pathol 60:311–320
• Southwell IA, Campbell MH (1991) Hypericin in different varieties of Hypericum perforatum in Australia. Phytochemistry 30:475–478
• Thanh NT, Murthy HN, Yu KW, Seung JC, Hahn EJ, Paek KY (2006) Effect of oxygen supply on cell growth and saponin production in bioreactor cultures of Panax ginseng. J Plant Physiol 163:1337
• Tolonen A, Hohtola A, Jalonen J (2003) Fast high-performance liquid chromatographic analysis of naphthodianthrones and phloroglucinols from Hypericum perforatum extracts. Phytochem Anal 14:306–309
• Verpoorte R, Contin A, Memelink J (2002) Biotechnology for the production of plant secondary metabolites. Phytochem Rev 1:13–25
• Walker TS, Pal Bais H, Vivanco JM (2002) Jasmonic acid-induced hypericin production in cell suspension cultures of Hypericum perforatum L. (St. John’s wort). Phytochemistry 60:289–293
• Yu KW, Gao WY, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215
• Zobayed S, Saxena PK (2004) Production of St. John’s wort plants under controlled environment for maximizing biomass and secondary metabolites. In Vitro Cell Dev Biol Plant 40:108–114

Identyfikatory

DOI

10.1007/s11738-013-1476-6