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2015 | 37 | 01 |
Tytuł artykułu

Effect of the vacuolar Naplus/Hplus antiporter transgene in a rice landrace and a commercial rice cultivar after its insertion by crossing

Treść / Zawartość
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Języki publikacji
EN
Abstrakty
EN
The vacuolar Na⁺/H⁺ antiporter is known to alleviate saline stress by sequestering Na⁺ in both wildtype Arabidopsis and rice and when over-expressed in many transgenic plants. Here we report on the effect of the NHX1 transgene on the salt tolerance properties it confers to a rice landrace and a commercial cultivar suitable for the dry winter season, but which suffers loss due to seasonal stresses, particularly in the coastal areas. The Nipponbare Na⁺/H⁺ antiporter 1.9 kb cDNA was cloned into pCAMBIA1305.1 under the control of the CaMV35S promoter and transformed into tissue-culture-responsive rice landrace Binnatoa (BA). The best-expressing transgenic line at T2 was found to be significantly tolerant at the seedling stage and was advanced to T3. The transgene was then transferred to the tissue-culture recalcitrant farmer-popular commercial rice genotype, BRRIdhan 28 (BR28) by crossing. The data generated both from semi-quantitative RT-PCR and western blot hybridization revealed that the transgene showed similar expression in the crossbred BR28 plants and BA transgenic line. Comparative stress tolerance tests, however, revealed that the BR28 crossbred lines were significantly less tolerant than its transgenic parent BA at both seedling and reproductive stages. A single successful transgenic event may therefore not show the same performance in the recipient genetic background, if introgressed by crossing.
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Wydawca
-
Rocznik
Tom
37
Numer
01
Opis fizyczny
Article: 1730 [10 p.], fig.,ref.
Twórcy
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
autor
  • Plant Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka 1000, Bangladesh
Bibliografia
  • Aldemita RR, Hodges TK (1996) Agrobacterium tumefaciens-mediated transformation of japonica and indica rice varieties. Planta 199:612–617
  • Amin M, Elias S, Hossain A, Ferdousi A, Rahman MS, Tuteja N, Seraj Z (2012) Over-expression of a DEAD-box helicase, PDH45, confers both seedling and reproductive stage salinity tolerance to rice (Oryza sativa L.). Mol Breed 30:345–354. doi:10.1007/s11032-011-9625-3
  • Apse MP, Aharon GS, Snedden WA, Blumwald E (1999) Salt tolerance conferred by overexpression of a vacuolar Na⁺/H⁺ antiport in Arabidopsis. Science 285:1256–1258
  • Apse MP, Sottosanto JB, Blumwald E (2003) Vacuolar cation/H⁺ exchange, ion homeostasis, and leaf development are altered in a T-DNA insertional mutant of AtNHX1, the Arabidopsis vacuolar Na⁺/H⁺ antiporter. Plant J 36:229–239
  • Barraga´n V et al (2012) Ion exchangers NHX1 and NHX2 mediate active potassium uptake into vacuoles to regulate cell turgor and stomatal function in Arabidopsis. Plant Cell (Online) 24:1127–1142
  • Bassil E et al (2011) The Arabidopsis Na⁺/H⁺ antiporters NHX1 and NHX2 control vacuolar pH and K? homeostasis to regulate growth, flower development, and reproduction. Plant Cell (Online) 23:3482–3497
  • Blumwald E, Poole RJ (1987) Salt tolerance in suspension cultures of sugar beet induction of Na⁺/H⁺ antiport activity at the tonoplast by growth in salt. Plant Physiol 83:884–887
  • Chen H, An R, Tang J-H, Cui X-H, Hao F-S, Chen J, Wang X-C (2007) Over-expression of a vacuolar Na⁺/H⁺ antiporter gene improves salt tolerance in an upland rice. Mol Breed 19:215–225
  • Cheng M, Lowe BA, Spencer TM, Ye X, Armstrong CL (2004) Factors influencing Agrobacterium-mediated transformation of monocotyledonous species. In Vitro Cell Dev Biol-Plant 40:31–45
  • Deinlein U, Stephan AB, Horie T, Luo W, Xu G, Schroeder JI (2014) Plant Salt-Toler Mech Trends Plant Sci 19:371–379
  • Doyle J (1991) DNA Protocols for Plants. In: Hewitt G, Johnston AB, Young JP (eds) Molecular techniques in taxonomy, vol 57. NATO ASI series. Springer Berlin Heidelberg, pp 283–293. doi:10.1007/978-3-642-83962-7_18
  • Fukuda A, Nakamura A, Tanaka Y (1999) Molecular cloning and expression of the Na⁺/H⁺ exchanger gene in Oryza sativa. Biochim Biophys Acta (BBA)-Gene Struct Expr 1446:149–155
  • Fukuda A, Nakamura A, Tagiri A, Tanaka H, Miyao A, Hirochika H, Tanaka Y (2004) Function, intracellular localization and the importance in salt tolerance of a vacuolar Na⁺/H⁺ antiporter from rice. Plant Cell Physiol 45:146–159
  • Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL, Fink GR (1999) The Arabidopsis thaliana proton transporters, AtNhx1 and Avp1, can function in cation detoxification in yeast. Proc Natl Acad Sci 96:1480–1485
  • Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the ‘‘gene-jockeying’’ tool. Microbiol Mol Biol Rev 67:16–37
  • Godfray HCJ et al (2010) Food security: the challenge of feeding 9 billion people. Science 327:812–818
  • Hossain M, Jaim W, Alam MS, Rahman AM (2013) Rice biodiversity in Bangladesh
  • Khanna HK, Raina SK (1999) Agrobacterium-mediated transformation of indica rice cultivars using binary and superbinary vectors. Funct Plant Biol 26:311–324
  • Kyozuka J, Otoo E, Shimamoto K (1988) Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet 76:887–890
  • Leidi EO et al (2010) The AtNHX1 exchanger mediates potassium compartmentation in vacuoles of transgenic tomato. Plant J 61:495–506
  • Li J-Y, He X-W XuL, Zhou J, Wu P, Shou H-X, Zhang F-C (2008) Molecular and functional comparisons of the vacuolar Na⁺/H⁺ exchangers originated from glycophytic and halophytic species. J Zhejiang Univ Sci B 9:132–140
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275
  • Maathuis FJ (2014) Sodium in plants: perception, signalling, and regulation of sodium fluxes. J Exp Bot 65:849–858
  • Maggio A, Zhu J-K, Hasegawa PM, Bressan RA (2006) Osmogenetics: aristotle to arabidopsis. Plant Cell (Online) 18:1542–1557
  • Moradi F, Ismail AM (2007) Responses of photosynthesis, chlorophyll fluorescence and ROS-scavenging systems to salt stress during seedling and reproductive stages in rice. Ann Bot 99:1161–1173
  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Ann Rev Plant Biol 59:651–681
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Nishi T, Yamada Y, Takahashi E (1973) The role of auxins in differentiation of rice tissues culture in vitro. J Plant Res 86:183–188
  • Ogawa T, Vernon LP, Mollenhauer HH (1969) Properties and structure of fractions prepared from Anabaena variabilis by the action of Triton X-100. Biochim Biophys Acta (BBA)-Bioenerget 172:216–229
  • Pardo JM, Cubero B, Leidi EO, Quintero FJ (2006) Alkali cation exchangers: roles in cellular homeostasis and stress tolerance. J Exp Bot 57:1181–1199
  • Rasul N, Ali K, Islam R, Seraj Z (1997) Transformation of an Indica Rice cultivar Binnatoa with Agrobacterium tumefaciens. Plant Tissue Cult 7:71–80
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: a laboratory manual, vol 1, 2nd edn. Cold Spring Harbor Laboratory Press
  • Seraj ZI, Islam Z, Faruque MO, Devi T, Ahmed S (1997) Identification of the regeneration potential of embryo derived calluses from various indica rice varieties. Plant Cell Tissue Organ Cult 48:9–13
  • Shoeb F, Yadav J, Bajaj S, Rajam M (2001) Polyamines as biomarkers for plant regeneration capacity: improvement of regeneration by modulation of polyamine metabolism in different genotypes of indica rice. Plant Sci 160:1229–1235
  • Wu Y-Y, Chen Q-J, Chen M, Chen J, Wang X-C (2005) Salt-tolerant transgenic perennial ryegrass (Lolium perenne L.) obtained by Agrobacterium tumefaciens-mediated transformation of the vacuolar Na⁺/H⁺ antiporter gene. Plant Sci 169:65–73
  • Xue Z-Y, Zhi D-Y, Xue G-P, Zhang H, Zhao Y-X, Xia G-M (2004) Enhanced salt tolerance of transgenic wheat (Tritivum aestivum L.) expressing a vacuolar Na⁺/H⁺ antiporter gene with improved grain yields in saline soils in the field and a reduced level of leaf Na⁺. Plant Sci 167:849–859
  • Yamaguchi T, Hamamoto S, Uozumi N (2013) Sodium transport system in plant cells Frontiers in plant science 4
  • Yokoi S, Quintero FJ, Cubero B, Ruiz MT, Bressan RA, Hasegawa PM, Pardo JM (2002) Differential expression and function of Arabidopsis thaliana NHX Na⁺/H⁺ antiporters in the salt stress response. Plant J 30:529–539
  • Yoshida S, Forno D, Cock J, Gomez K (1976) Routine procedure for growing rice plants in culture solution. Laboratory manual for physiological studies of rice, pp 61–66
  • Zhang H-X, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765–768
  • Zhang H-X, Hodson JN, Williams JP, Blumwald E (2001) Engineering salt-tolerant Brassica plants: characterization of yield and seed oil quality in transgenic plants with increased vacuolar sodium accumulation. Proc Natl Acad Sci 98:12832–12836
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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