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2014 | 61 | 1 |

Tytuł artykułu

Activation of MAP kinase pathways in Galleria mellonella infected with Bacillus thuringiensis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
We followed changes in the level of phospho-MAP kinases in the greater wax moth Galleria mellonella after infection with Bacillus thuringiensis. We observed an enhanced level of phosphorylated p38 and JNK in fat bodies of the infected larvae. In hemocytes, injection of B. thuringiensis caused the highest increase in phospho-JNK, however, all pathways were activated after aseptic injection. We report that Galleria mellonella larvae exposed to heat shock before infection showed an enhanced level of phosphorylated JNK in fat body. This finding is relevant in the light of our previous reports, which submit evidence that pre-shocked animals are more resistant to infection.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

61

Numer

1

Opis fizyczny

p.185-189,fig.,ref.

Twórcy

autor
  • Department of Immunobiology, Institute of Biology and Biochemistry, Faculty of Biology and Biotechnology, Lublin, Poland
autor
  • Department of Immunobiology, Institute of Biology and Biochemistry, Faculty of Biology and Biotechnology, Lublin, Poland
  • Department of Immunobiology, Institute of Biology and Biochemistry, Faculty of Biology and Biotechnology, Lublin, Poland

Bibliografia

  • Arrese EL, Soulages JL (2010) Insect fat body: energy, metabolism, and regulation. Annu Rev Entomol 55: 207-225. 
  • Boutros M, Agaisse H, Perrimon N (2002) Sequential activation of signaling pathways during innate immune responses in Drosophila. Dev Cell 3: 711-722. 
  • Boutros L, Chevet E, Metrakos P (2008) Mitogen-activated protein (MAP) kinase/MAP kinase phosphatase regulation: roles in cell growth, death, and cancer. Pharmacol Rev 60: 261-310. 
  • Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Anal Biochem 72: 248. 
  • Charroux B, Rival T, Narbonne-Reveau K, Royet, J (2009) Bacterial detection by Drosophila peptidoglycan recognition proteins. Microbes Infect 11: 631-636. 
  • Glavis-Bloom J, Muhammed M, Mylonakis E (2012) Of model hosts and man: using Caenorhabditis elegans, Drosophila melanogaster and Galleria mellonella as model hosts for infectious disease research. Adv Exp Med Biol 710: 11-17. 
  • Han ZS, Enslen H, Hu X, Meng X, Wu IH, Barrett T, Davis RJ, Ip YT (1998) A conserved p38 mitogen-activated protein kinase pathway regulates Drosophila immunity gene expression. Mol Cell Biol 18: 3527-3539. 
  • Kallio J, Leinonen A, Ulvila J, Valanne S, Ezekowitz RA, Rämet M (2005) Functional analysis of immune response genes in Drosophila identifies JNK pathway as a regulator of antimicrobial peptide gene expression in S2 cells. Microbes Infect 7: 811-819. 
  • Kaneko T, Goldman WE, Mellroth P, Steiner H, Fukase K, Kusumoto S, Harley W, Fox A, Golenbock D, Silverman N (2004) Monomeric and polymeric gram-negative peptidoglycan but not purified LPS stimulate the Drosophila IMD pathway. Immunity 20: 637-649. 
  • Khush RS, Leulier F, Lemaitre B (2001) Drosophila immunity: two paths to NF-κB. Trends in Immunol 22: 260-264. 
  • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685. 
  • Lemaitre B, Kromer-Metzger E, Michaut L, Nicolas E, Meister M, Georgel P, Reichhart JM, Hoffmann JA (1995) A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defence. Proc Nat Acad of Sci USA 92: 9465-9469. 
  • Lemaitre B, Nicolas E, Michaut L, Reichhart JM, Hoffmann JA (1996) The dorsoventral regulatory gene cassette spätzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 86: 973-983. 
  • Li Y, Xiang Q, Zhang Q, Huang Y, Su Z (2012) Overview on the recent study of antimicrobial peptides: origins, functions, relative mechanisms and application. Peptides 37: 207-215. 
  • Williams MJ (2007) Drosophila hemopoiesis and cellular immunity. J Immunol 178: 4711-4716. 
  • Wojda I, Kowalski P, Jakubowicz T (2004) JNK MAP kinase is involved in the humoral immune response of the greater wax moth Galleria mellonella. Arch Insect Biochem Physiol 56: 143-54. 
  • Wojda I, Kowalski P, Jakubowicz T (2009) Humoral immune response of Galleria mellonella larvae after infection by Beauveria bassiana under optimal and heat-shock conditions. J Insect Physiol 55: 525-531. 
  • Wojda I (2010) Heat shock and the susceptibility of organisms to infection and osmotic challenge. Postepy Biochem 56: 83-94 (in Polish). 
  • Wojda I (2012) MAP kinase pathways- their evolution and role in some neurodegenerative diseases. Postepy Biochem 58: 79-90 (in Polish). 
  • Wojda I, Taszłow P (2013) Heat shock affects host-pathogen interaction in Galleria mellonella infected with Bacillus thuringiensis. J Insect Physiol 59: 894-905. 
  • Zhang YL, Dong C (2005) MAP kinases in immune responses. Cell Mol Immunol. 2: 20-27. 

Typ dokumentu

Bibliografia

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Identyfikator YADDA

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