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2013 | 35 | 03 |
Tytuł artykułu

Novel isoforms of proteinaceous a-amylase inhibitor (a-AI) from seed extract of Albizia lebbeck

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Proteinaceous inhibitors of digestive α-amylase occur naturally in leguminous seeds and find applications in agriculture and clinical studies. We have detected and isolated eight novel α-amylase inhibitor isoforms in the seed extract of Albizia lebbeck. They are designated as ALαAI- 1 to AL-αAI-8. These isoforms specifically inhibit human salivary a-amylase and porcine pancreatic α-amylase. The occurrence and profile of α-amylase inhibitor isoforms were revealed by 7 % native-PAGE containing 0.1 % starch. The apparent molecular weights of native bands of AL-αAIs were 97.4, 68.6, 61.0, 57.2, 56.0, 54.7, 51.1, and 47.7 kDa, respectively. Partial purification of potent a-amylase inhibitor was achieved using ammonium sulfate fractionation and gel filtration chromatography on G-100 Sephadex column followed by preparative gel electrophoresis. SDS-PAGE analysis of partially purified AL-αAI showed two polypeptide bands of *35.8 and *32.6 kDa. All these isoforms showed effective resistance to in vitro proteolysis by pepsin, trypsin, and chymotrypsin. These inhibitors are stable over a wide range of pH and temperature and have optimum activity at pH 7 and at 37 C. The finding and information obtained in the present investigation about novel isoforms of α-amylase inhibitors from A. lebbeck could be important and may find applications in clinical studies to modulate starch digestion and glycemic index.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
35
Numer
03
Opis fizyczny
p.901-909,fig.,ref.
Twórcy
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
autor
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
  • Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad, India
Bibliografia
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