Development of integrated multi-electrode probe to study ion transport across epithelial cell layer

• Autorzy: Dolowy K. , Lewenstam A. , Toczylowska-Maminska R. , Zajac M. , Madej H. , Jarzabek W.
• Języki publikacji: EN

Abstrakty

EN

Our present construction of multisensory system is a result of six years of mostly unsuccessful attempts. At first, we had try to grow cells on round filter and then sandwich filter with cells between to flat multiprobe electrodes. This failed because handling the thin and flexible filter during assembling of the device usually damaged the cell monolayer. Secondly, we tried to exchange the medium by the tubes parallel to the cells plane. This failed due to difficulties in sealing of the system. Thirdly, we tried to use solid state electrodes. This attempt also failed because working electrodes stick over the surface of the probe by more than 100 µm.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2014
• Tom: 74
• Numer: 3
• Opis fizyczny: p.331-332

Twórcy

autor

Dolowy K.

• Department of Biophysics, Warsaw Life Sciences University – SGGW, Warsaw, Poland

autor

Lewenstam A.

• Centre for Process Analytical Chemistry and Sensor Technology (ProSens), Process Chemistry Centre, Åbo- Turku, Finland
• AGH - University of Science and Technology, Faculty of Materials Science and Ceramics, Krakow, Poland

autor

Toczylowska-Maminska R.

• Department of Biophysics, Warsaw Life Sciences University – SGGW, Warsaw, Poland

autor

Zajac M.

• Department of Biophysics, Warsaw Life Sciences University – SGGW, Warsaw, Poland

autor

Madej H.

• Department of Biophysics, Warsaw Life Sciences University – SGGW, Warsaw, Poland

autor

Jarzabek W.

• Department of Biophysics, Warsaw Life Sciences University – SGGW, Warsaw, Poland