Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2012 | 72 | 2 |
Tytuł artykułu

Protective effect of treatment with black cumin oil on spatial cognitive functions of rats that suffered global cerebrovascular hypoperfusion

Warianty tytułu
Języki publikacji
The fixed oil of black cumin seeds, Nigella sativa L. (NSO), has shown considerable antioxidant and anti-inflammatory activities. Chronic cerebral hypoperfusion has been linked to neurodegenerative disorders including Alzheimer's disease (AD) and its subsequent cognitive impairment in which oxidative stress and neuroinflammation are the principal culprits. Cerebrovascular hypoperfusion was experimentally achieved by bilateral common carotid arteries occlusion (2VO) in rats. Morris water maze (MWM) test was employed to assess the effects of NSO on spatial cognitive function before and after 2VO intervention. Rats were divided into long-term memory (LTM) and short-term memory (STM) groups, each was further subdivided into 3 subgroups: sham control, untreated 2VO and NSO treated 2VO group. All subgroups were tested with MWM at the tenth postoperative week. Working memory test results for both sham control and NSO treated groups showed significantly lower escape latency time and total distance travelled than untreated 2VO group. Similarly, LTM and STM MWM tests for sham control and NSO treated groups revealed significantly better maze test performance as compared to untreated 2VO group. Sham control and NSO treated 2VO groups demonstrated superior probe memory test performance as compared to untreated 2VO group. The fixed oil of Nigella sativa seeds has demonstrated noticeable spatial cognitive preservation in rats challenged with chronic cerebral hypoperfusion which indicates a promising prospective neuroprotective effect.
Słowa kluczowe
Opis fizyczny
  • Department of Basic Medical Sciences, Faculty of Medicine, Malaysia
  • Department of Basic Medical Sciences, Faculty of Medicine, Malaysia
  • Department of Basic Medical Sciences, Faculty of Medicine, Malaysia
  • Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, Malaysia
  • Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, Malaysia
  • Ahmadiasl N, Alaei H, Hanninen O (2003) Effect of exer¬cise on learning, memory and levels of epinephrine in rats hippocampus. J Sport Sci Med 2: 106-109.
  • Aizenstein HJ, Nebes RD, Saxton JA, Price JC, Mathis CA, Tsopelas ND, Ziolko SK, James JA, Snitz BE, Houck PR (2008) Frequent amyloid deposition without significant cognitive impairment among the elderly. Arch Neurol 65: 1509-1517.
  • Al Mofleh IA, Alhaider AA, Mossa JS, Al-Sohaibani MO, Al-Yahya MA, Rafatullah S, Shaik SA (2008) Gastroprotective effect of an aqueous suspension of black cumin Nigella sativa on necrotizing agents-induced gas¬tric injury in experimental animals. Saudi J Gastroenterol 14: 128-134.
  • Baddeley A, Bressi S, Della Sala S, Logie R, Spinnler H (1991) The decline of working memory in Alzheimer's disease. Brain 114: 2521-2542.
  • Barondes SH, Cohen HD (1968) Arousal and the conversion of' short-term" to" long-term" memory. Proceedings of the National Acad Sci U S A 61: 923-929.
  • Bayrak O, Bavbek N, Karatas OF, Bayrak R, Catal F, Cimentepe E, Akbas A, Yildirim E, Unal D, Akcay A (2008) Nigella sativa protects against ischaemia/reperfu- sion injury in rat kidneys. Nephrol Dial Transplant 23: 2206-2212.
  • Cechetti F, Worm P, Pereira L, Siqueira I (2010) The modi¬fied 2VO ischemia protocol causes cognitive impairment similar to that induced by the standard method, but with a better survival rate. Braz J Med Biol Res 43: 1178¬1183.
  • Choi BR, Lee SR, Han JS, Woo SK, Kim KM, Choi DH, Kwon KJ, Han SH, Shin CY, Lee J, Chung CS, Kim HY (2011) Synergistic memory impairment through the inter¬action of chronic cerebral hypoperfusion and amlyloid toxicity in a rat model. Stroke 42: 2595-2604.
  • Conrad CD (2010) A critical review of chronic stress effects on spatial learning and memory. Prog Neuro- Psychopharmacol Biol Psychiatry 34: 742-755.
  • De Jong GI, Farkas E, Stienstra CM, Plass JR, Keijser JN, de la Torre JC, Luiten PG (1999) Cerebral hypoperfusion yields capillary damage in the hippocampal CA1 area that correlates with spatial memory impairment. Neuroscience 91: 203-210.
  • de la Torre JC (2010a) Alzheimer's disease is incurable but preventable. J Alzheimer's Dis 20: 861-870.
  • de la Torre JC (2010b) The vascular hypothesis of Alzheimer's disease: bench to bedside and beyond. Neuro-Deg Dis 7: 116-121.
  • de la Torre JC (2010c) Vascular risk factor detection and control may prevent Alzheimer's disease. Ageing Res Rev 9: 218-225.
  • de la Torre JC, Aliev G (2005) Inhibition of vascular nitric oxide after rat chronic brain hypoperfusion: spatial mem¬ory and immunocytochemical changes. J Cereb Blood Flow Metab 25: 663-672.
  • de Wilde MC, Farkas E, Gerrits M, Kiliaan AJ, Luiten PGM (2002) The effect of n-3 polyunsaturated fatty acid-rich diets on cognitive and cerebrovascular parameters in chronic cerebral hypoperfusion. Brain Res 947: 166-173.
  • El-Naggar T, Gomez-Serranillos MP, Palomino OM, Arce C, Carretero ME (2010) Nigella sativa L. seed extract modu¬lates the neurotransmitter amino acids release in cultured neurons in vitro. J Biomed Biotechnol 2010: 398312.
  • Erkan N, Ayranci G, Ayranci E (2008) Antioxidant activities of rosemary (Rosmarinus Officinalis L.) extract, black- seed (Nigella sativa L.) essential oil, carnosic acid, ros- marinic acid and sesamol. Food Chem 110: 76-82.
  • Farkas E, Luiten PGM, Bari F (2007) Permanent, bilateral common carotid artery occlusion in the rat: a model for chronic cerebral hypoperfusion-related neurodegenera- tive diseases. Brain Res Rev 54: 162-180.
  • Giannakopoulos P, Herrmann F, Bussiere T, Bouras C, Kovari E, Perl D, Morrison J, Gold G, Hof P (2003) Tangle and neuron numbers, but not amyloid load, predict cognitive status in Alzheimer's disease. Neurology 60: 1495-1500.
  • Holmes C, Boche D, Wilkinson D, Yadegarfar G, Hopkins V, Bayer A, Jones RW, Bullock R, Love S, Neal JW (2008) Long-term effects of A [beta] 42 immunisation in Alzheimer's disease: follow-up of a randomised, placebo- controlled phase I trial. Lancet 372: 216-223.
  • Hosseinzadeh H, Parvardeh S, Asl MN, Sadeghnia HR, Ziaee T (2007) Effect of thymoquinone and Nigella sativa seeds oil on lipid peroxidation level during global cere¬bral ischemia-reperfusion injury in rat hippocampus. Phytomedicine 14: 621-627.
  • Ismail N, Ismail M, Latiff LA, Mazlan M, Mariod AA (2008) Black Cumin Seed (Nigella Sativa Linn.) Oil and Its Fractions Protect Against Beta Amyloid Peptide Induced Toxicity in Primary Cerebellar Granule Neurons. J Food Lipids 15: 519-533.
  • Kanter M, Coskun O, Korkmaz A, Oter S (2004) Effects of Nigella sativa on oxidative stress and cell damage in streptozotocin induced diabetic rats. The Anat Rec A Discov Mol Cell Evol Biol 279: 685-691.
  • Kanter M (2008a) Nigella sativa and derived thymoquinone prevents hippocampal neurodegeneration after chronic toluene exposure in rats. Neurochem Res 33: 579-588.
  • Kanter M (2008b) Protective effects of Nigella sativa on the neuronal injury in frontal cortex and brain stem after chronic toluene exposure. Neurochem Res 33: 2241¬2249.
  • Kim SK, Cho KO, Kim SY (2008) White matter damage and hippocampal neurodegeneration induced by permanent bilateral occlusion of common carotid artery in the rat: comparison between wistar and spraguedawley strain. Korean J Physiol Pharmacol 12: 89-94.
  • Liu HX, Zhang JJ, Zheng P, Zhang Y (2005) Altered expres¬sion of MAP-2, GAP-43, and synaptophysin in the hip¬pocampus of rats with chronic cerebral hypoperfusion correlates with cognitive impairment. Brain Res Mol Brain Res 139: 169-177.
  • Maei HR, Zaslavsky K, Teixeira CM, Frankland PW (2009) What is the most sensitive measure of water maze probe test performance? Front Integr Neurosci 3: 33.
  • Marosi M, Rakos G, Robotka H, Nemeth H, Sas K (2006) Hippocampal (CA1) activities in Wistar rats from differ¬ent vendors:: Fundamental differences in acute ischemia. J Neurosci Methods 156: 231-235.
  • McGaugh JL (2000) Memory - a century of consolidation. Science 287: 248.
  • Milionis HJ, Florentin M, Giannopoulos S (2008) Metabolic syndrome and Alzheimer's disease: a link to a vascular hypothesis. CNS Spectr 13: 606-613.
  • Morris R (1984) Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 11: 47-60.
  • Nakazawa K, McHugh TJ, Wilson MA, Tonegawa S (2004) NMDA receptors, place cells and hippocampal spatial memory. Nat Rev Neurosci 5: 361-372.
  • Nunez J (2008) Morris water maze experiment. J Vis Exp 19: 897. (doi: 10.3791/897)
  • Obrenovich ME, Smith MA, Siedlak SL, Chen SG, De La Torre JC, Perry G, Aliev G (2006) Overexpression of GRK2 in Alzheimer disease and in a chronic hypoperfu- sion rat model is an early marker of brain mitochondrial lesions. Neurotox Res 10: 43-56.
  • Obrenovich ME, Palacios HH, Gasimov E, Leszek J, Aliev G (2009) The GRK2 overexpression is a primary hall¬mark of mitochondrial lesions during early Alzheimer disease. Cardiovasc Psychiatry Neurol 2009: 327360. (doi: 10.1155/2009/327360)
  • Pappas B, De La Torre J, Davidson C, Keyes M, Fortin T (1996) Chronic reduction of cerebral blood flow in the adult rat: late-emerging CA1 cell loss and memory dys¬function. Brain Res 708: 50-58.
  • Peng Y, Xu S, Chen G, Wang L, Feng Y, Wang X (2007) l-3- n-Butylphthalide improves cognitive impairment induced by chronic cerebral hypoperfusion in rats. J Pharmacol Exp Ther 321: 902-910.
  • Randhawa MA (2008) Black seed, Nigella sativa, deserves more attention. J Ayub Med Coll Abbottabad 20: 1-2.
  • Reisberg B, Franssen E, Shah MA, Weigel J, Bobinski M, Wisniewski HM (2002) Clinical diagnosis of dementia. In: Dementia (2nd ed.) (Maj M, Sartorius N, Eds). John Wiley, Chichester, UK, p. 69-74.
  • Salem ML (2005) Immunomodulatory and therapeutic prop¬erties of the Nigella sativa L. seed. International Immunopharmacol 5: 1749-1770.
  • Sivilia S, Giuliani A, Fernández M, Turba M, Forni M, Massella A, De Sordi N, Giardino L, Calzà L (2009) Intravitreal NGF administration counteracts retina degen¬eration after permanent carotid artery occlusion in rat. BMC Neurosci 10: 52.
  • Stevens WD, Fortin T, Pappas BA (2002) Retinal and optic nerve degeneration after chronic carotid ligation. Stroke 33: 1107-1112.
  • Takizawa S, Fukuyama N, Hirabayashi H, Kohara S, Kazahari S, Shinohara Y, Nakazawa H (2003) Quercetin, a natural flavonoid, attenuates vacuolar formation in the optic tract in rat chronic cerebral hypoperfusion model. Brain Res 980: 156-160.
  • Vorhees CV, Williams MT (2006) Morris water maze: pro¬cedures for assessing spatial and related forms of learning and memory. Nat Prot 1: 848-858.
  • Wakita H, Tomimoto H, Akiguchi I, Lin JX, Ihara M, Ohtani R, Shibata M (2003) Ibudilast, a phosphodiesterase inhibitor, protects against white matter damage under chronic cerebral hypoperfusion in the rat. Brain Res 992: 53-59.
  • Weinstock M, Shoham S (2004) Rat models of dementia based on reductions in regional glucose metabolism, cere¬bral blood flow and cytochrome oxidase activity. J Neural Transm 111: 347-366.
  • Wu ZL, Thomas SA, Villacres EC, Xia Z, Simmons ML, Chavkin C, Palmiter RD, Storm DR (1995) Altered behavior and long-term potentiation in type I adenylyl cyclase mutant mice. Proc Natl Acad Sci U S A 92: 220-224.
  • Yildiz F, Coban S, Terzi A, Ates M, Aksoy N, Cakir H, Ocak AR, Bitiren M (2008) Nigella sativa relieves the deleteri¬ous effects of ischemia reperfusion injury on liver. World J Gastroenterol 14: 5204-5209.
  • Zaoui A, Cherrah Y, Mahassini N, Alaoui K, Amarouch H, Hassar M (2002) Acute and chronic toxicity of Nigella sativa sativa fixed oil. Phytomedicine 9: 69-74.
  • Zhang M, Moon C, Chan GCK, Yang L, Zheng F, Conti AC, Muglia L, Muglia LJ, Storm DR, Wang H (2008) Ca-stimulated type 8 adenylyl cyclase is required for rapid acquisition of novel spatial information and for working/episodic-like memory. J Neurosci 28: 4736¬4744.
Rekord w opracowaniu
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.