IL-1betta stimulates cytokine secretion by peripheral leukocytes following subarachnoid hemorrhage in rats.

• Autorzy: Jedrzejowska-Szypulka H. , Woszczycka-Korczynska L. , Olakowska E. , Larysz-Brysz M. , Lewin-Kowalik J.
• Języki publikacji: EN

Abstrakty

EN

Much evidence indicates that pathologies observed after subarachnoid hemorrhage (SAH), including vasospasm and neurological defects, are the effects of immuno-infl ammatory processes accompanying the hemorrhage. Following SAH, CSF concentration of cytokines increase, what may change the cerebral perfusion and destroy brain–blood barrier. These cytokines are produced by activated leukocytes as well as hypoxic cells in the brain. The aim of this study was to examine the role of IL-1β in stimulation of peripheral leukocytes to increase secretion of cytokines after SAH. SAH was produced by injection of 150 μL of autologous arterial blood into cisterna magna. In 50% of animals, IL-1β activity was inhibited by intracerebroventricular administration of anti-rat IL-1β antibodies (SAH’ groups). Control group consisted of sham-operated rats. Ninety minutes or 24 h following surgery, blood samples were collected and subpopulations of mononuclear leukocytes were isolated and cultured for 24 h. Then, the concentration of TNF-α, ET-1 and IL-6 in supernatants was measured by means of ELISA. SAH resulted in signifi cant increase in TNF-α and ET-1, especially after 24 h, and no change in IL-6 concentration. Inhibition of IL1-β decreased the secretion of TNF-α and ET-1 and led to increase in IL-6. We found that peripheral leukocytes are an additional source of cytokines following SAH, and their activation depends on IL1-β produced after hemorrhage.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2009
• Tom: 69
• Numer: 3
• Opis fizyczny: p.311

Twórcy

autor

Jedrzejowska-Szypulka H.

• Department of Physiology, Medical University of Silesia, Katowice, Poland

autor

Woszczycka-Korczynska L.

• Department of Physiology, Medical University of Silesia, Katowice, Poland

autor

Olakowska E.

• Department of Physiology, Medical University of Silesia, Katowice, Poland

autor

Larysz-Brysz M.

• Department of Physiology, Medical University of Silesia, Katowice, Poland

autor

Lewin-Kowalik J.

• Department of Physiology, Medical University of Silesia, Katowice, Poland