Characterization of catechol 2,3-dioxygenase from Planococcus sp. strain S5 induced by high phenol concentration

• Autorzy: Hupert-Kocurek K. , Guzik U. , Wojcieszynska D.
• Języki publikacji: EN

Abstrakty

EN

 This study aimed at characterization of a new catechol 2,3-dioxygenase isolated from a Gram-positive bacterium able to utilize phenol as the sole carbon and energy source. Planococcus sp. strain S5 grown on 1 or 2 mM phenol showed activity of both a catechol 1,2- and catechol 2,3-dioxygenase while at a higher concentrations of phenol only catechol 2,3-dioxygenase activity was observed. The enzyme was optimally active at 60°C and pH 8.0. Kinetic studies showed that the Km and Vmax of the enzyme were 42.70 µM and 329.96 mU, respectively. The catechol 2,3-dioxygenase showed the following relative meta-cleavage activities for various catechols tested: catechol (100%), 3-methylcatechol (13.67%), 4-methylcatechol (106.33%) and 4-chlorocatechol (203.80%). The high reactivity of this enzyme towards 4-chlorocatechol is different from that observed for other catechol 2,3-dioxygenases. Nucleotide sequencing and homology search revealed that the gene encoding the S5 catechol 2,3-dioxygenase shared the greatest homology with the known genes encoding isoenzymes from Gram-negative Pseudomonas strains.

Słowa kluczowe

EN

catechol 2,3-dioxygenase; Planococcus; high phenol concentration; phenol degradation; cis,cis-muconic acid; mineral salt medium

• Wydawca: -
• Czasopismo: Acta Biochimica Polonica
• Rocznik: 2012
• Tom: 59
• Numer: 3
• Opis fizyczny: p.345-351,fig.,ref.

Twórcy

autor

Hupert-Kocurek K.

• Department of Biochemistry, Faculty of Biology and Environmental Protection, University of Silesia in Katowice, Katowice, Poland

autor

Guzik U.

autor

Wojcieszynska D.

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