Effect of rhizobacterial-mediated auxin on growth promotion of wheat and mung bean plant
The use of beneficial rhizobacteria as biofertilizers are best alternatives of chemical fertilizers and might be potential tools for sustainable agriculture. Plant growth promoting rhizobacteria (PGPR) increased crop production by using different mechanisms. Auxin, bacterial phytohormone, plays a significant role in cell elongation, cell division and cell differentiation of plants. The aim of our study was to investigate the native valuable rhizobacteria for growth promotion of wheat and Mung bean plants. The bacterial isolates from different regions of southern Punjab were screened on the basis of their auxin production potential. The auxin production ability was found to be up to 152 ug/ml. Out of 40 strains, 58.8% were gram-positive rods and the rest were gram negative. All the strains were evaluated for their plant growth-promoting potential while being applied on wheat and Mung bean plants, and observed their effect on plant growth promotion under laboratory conditions. These strains showed significant increases (p = 0.05) in root length, shoot length, dry and fresh weight of wheat and mung bean seedlings as compared to control. Our results indicated that PGPR are the best alternatives of chemical fertilizers and have more capability to increase plant growth and yield. It is a cheaper way to accomplish the increasing demand for food.
- 1. NEHRA V., CHOUDHARY M. A review on plant growth promoting rhizobacteria acting as bioinoculants and their biological approach towards the production of sustainable agriculture. J. Appl. & Nat. Sci. 7, 540, 2015.
- 2. GLICK B.R. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiol. Res. 169, 30, 2014.
- 3. NANDAL M., HOODA R. Plant growth promoting rhizobacteria: a review article. IJCR. 12, 3863, 2013.
- 4. GAMALERO E., GLICK B.R. Mechanisms used by plant growth-promoting bacteria. In “Bacteria in Agrobiology: Plant Nutrient Management” Springer. 17, 46, 2011 .
- 5. AHEMAD M., KIBRET M. Mechanisms and applications of plant growth promoting rhizobacteria. current perspective. J. King. Sau. Univ. Sci. 26, 1, 2014.
- 6. SPAEPEN S., VANDERLEYDEN J., REMANS R. Indole-3-acetic acid in microbial and microorganism-plant signaling. FEMS. Microbiol. Rev. 4, 425, 2007.
- 7. ZAIDI A., KHAN M.S., AHEMAD M., OVES M. Plant grows promotion by phosphate solubilizing bacteria. Acta. Microbiol. Immunol. Hung. 56, 263, 2009.
- 8. NELSON L.M. Plant growth promoting rhizobacteria (PGPR):Prospects for new inoculants. Online. Crop Management, 2004.
- 9. KHAKIPOUR N., KHAVAZI K., PAZIRA E., ASADIRAHMANI H. Production of Auxin Hormone by Fluorescent Pseudomonads. American-Eurasian J. Agric. & Environ. Sci. 4, 687, 2008.
- 10. A SHOEBITZ M., RIBAUDO C.M., PARDO M.A., CANTORE M.L., CIAMPI L., CURA J.A. Plant growth promoting properties of a strain of Enterobacter ludwigii isolated from Lolium perennerhizosphere. Soil. Biol. Biochem. 41, 1768, 2009.
- 11. ZIZ K., NAWAZ M., NAZIR J., ANJUM A.A., YAQUB T., AHMAD D.U.M., REHAN U.M., AZIZ G., KHAN M. Isolation, characterization and effect of auxin producing bacteria on growth of triticum aestivum. J. Anim. Plant. Sci. 25, 1003,2015.
- 12. ALI B., SABRI N.A., LJUNG K., HASNAIN S. Quantification of indole-3-acetic acid from plant associated Bacillus spp. and their phytostimulatory effect on Vigna radiata (L.). World. J. Microbiol. Biotechnol. 25, 519, 2009.
- 13. SARWAR M.A., IBRAHIM M., TAHIR M., AHMAD K., KHAN Z.I., VALEEM E.E. Appraisal of pressmud and inorganic fertilizers on soil properties, yield and sugarcane quality. Pak. J. Bot. 42, 1361, 2010.
- 14. IQBAL A., HASNAIN S. Auxin Producing Pseudomonas Strains: Biological Candidates to Modulate the Growth of Triticum aestivum Beneficially. Am. J. Plant. Sci, 4, 1693, 2013.
- 15. CASSÁN F, VANDERLEYDEN J., SPAEPEN S. Physiological and agronomical aspects of phytohormone production by model plant-growth-promoting rhizobacteria (PGPR) Belonging to the Genus Azospirillum. J. Plant. Growth. Regul. 33, 440, 2014.
- 16. HUSSAIN A., HASNAIN S. Interactions of bacterial cytokinins and IAA in the rhizosphere may alter phytostimulatory efficiency of rhizobacteria. World. J. Microbiol. Biotechnol. 27, 2645, 2011.
- 17. MINAX I., SAXENA J. Disease suppression and crop improvement in moong beans (Vigna radiata) through Pseudomonas and Burkholderi as trains isolated from semi arid region of Rajasthan, India. Bio.Cont. 55, 799, 2010.
- 18. VASUDEVAN P., REDDY S.M., KAVITHA S., VELUSAMY P., PAULRA J., DAVID S.R., PURUSHOTHAMAN M.S., PRIYADARSIN I., BRINDHA V., BHARATHKUMAR S., KLOEPPER W.J., GNANAMANICKAM S.S. Role of biological preparations in enhancement of rice seedling growth and grain yield. Current Science, 83, 1140, 2002.